Pulsed Field Gel Electrophoresis typing of human and retail foodstuff Campylobacters: An Irish perspective

O'Leary, A.; Whyte, Paul; Madden, Robert H.; Cormican, Martin; Moore, John E.; Namara, Eleanor Mc; Gill, K Mc; Kelly, Lorraine; Cowley, D.; Moran, L.; Scates, Pam; Collins, John D.; Carroll, C.

doi:10.1016/j.fm.2010.10.003

Cited by 12 publications

(6 citation statements)

References 31 publications

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“…Though 16S rDNA sequencing is adequate to assign genus or species to an isolate, in most cases it is not sufficient for refined typing, e.g., epidemiological studies. PFGE, can be applied for high resolution typing, but is generally inappropriate for assigning genus or species to microorganisms ( O’Leary et al, 2011 ).…”

Section: Applications In Microbial Diagnosismentioning

confidence: 99%

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis

et al. 2015

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Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial identification and diagnosis. During the MALDI-TOF MS process, microbes are identified using either intact cells or cell extracts. The process is rapid, sensitive, and economical in terms of both labor and costs involved. The technology has been readily imbibed by microbiologists who have reported usage of MALDI-TOF MS for a number of purposes like, microbial identification and strain typing, epidemiological studies, detection of biological warfare agents, detection of water- and food-borne pathogens, detection of antibiotic resistance and detection of blood and urinary tract pathogens etc. The limitation of the technology is that identification of new isolates is possible only if the spectral database contains peptide mass fingerprints of the type strains of specific genera/species/subspecies/strains. This review provides an overview of the status and recent applications of mass spectrometry for microbial identification. It also explores the usefulness of this exciting new technology for diagnosis of diseases caused by bacteria, viruses, and fungi.

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Section: Applications In Microbial Diagnosismentioning

confidence: 99%

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis

et al. 2015

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“…MALDI-TOF MS is used for clinical bacteriology, 151,152 detecting antibiotic resistance bacteria 153 and identifying different strains. 154 In aquaculture, this technology has been studied in different viruses, 155,156 bacteria, [157][158][159][160][161][162] fungus 163 and parasite. 164 in molluscs.…”

Section: Clustered Regularly Interspaced Short Palindromic Repeats-ba...mentioning

confidence: 99%

Emerging technologies revolutionising disease diagnosis and monitoring in aquatic animal health

Bohara,

Joshi,

Acharya

et al. 2023

Reviews in Aquaculture

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In recent years, aquaculture has seen tremendous growth worldwide due to technological advancements, leading to research and development of various innovations. Aquaculture farmers prioritise early diagnosis for timely treatment to achieve better productive and economic performance. Aquatic animal health experts still employ traditional diagnostic methods using visual diagnosis, cell culture, media culture, histopathology and serology. However, the developments of technologies in aquamedicine, such as sequencing, biosensors and CRISPR, have enabled rapid disease detection within minutes. Furthermore, integrating sensors, drones, artificial intelligence and the internet in aquaculture farm monitoring has helped farmers take decisive actions to improve production. Advancements in diagnostic techniques have significantly enhanced the efficient detection of bacterial, viral, parasitic and fungal diseases in aquatic animals. Moreover, monitoring water quality, aquatic animal health and animal behaviour on farms has become exceptionally streamlined with cutting‐edge tools like drones, sensors and artificial intelligence. Summarising research and development in aquatic animal health and monitoring aids efficient technology adoption in aquaculture. With these advanced technologies' continued development and adoption in developed countries, the aquaculture industry is experiencing growth and increased efficiency, benefiting farmers and consumers in these regions. However, farmers and educators in developing countries lack information about these technologies. Training of agricultural educators and efficient dissemination of knowledge and technologies through advertising and publication in collaboration with companies is essential. This review delves into emerging technologies capable of replacing the conventional diagnostic and monitoring methods utilised in aquaculture. We also explore their strengths, limitations and potential future applications within aquaculture settings.

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“…Several molecular typing methods have been used for this purpose (Fry et al, 2000;Wassenaar & Newell, 2000), amongst which macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) has been extensively used for molecular typing of thermophilic Campylobacter spp. (Ribot et al, 2001;Praakle-Amin et al, 2007;Lyhs et al, 2010;O'Leary et al, 2011). PFGE is generally accepted as one of the best discriminatory typing methods currently available for the subtyping of Campylobacter spp., and has provided important information regarding the genotypic diversity and epidemiology of this genus (Olsen et al, 2001a, b).…”

Section: Introductionmentioning

confidence: 99%

Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination amongst environmental, human, animal and food sources

Gomes

Souza

Passaglia

et al. 2016

Journal of Medical Microbiology

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Campylobacter coli and Campylobacter jejuni are two of the most common causative agents of food-borne gastroenteritis in numerous countries worldwide. In Brazil, campylobacteriosis is underdiagnosed and under-reported, and few studies have molecularly characterized Campylobacter spp. in this country. The current study genotyped 63 C. coli strains isolated from humans (n512), animals (n521), food (n510) and the environment (n520) between 1995 and 2011 in Brazil. The strains were genotyped using pulsed-field gel electrophoresis (PFGE), sequencing the short variable region (SVR) of the flaA gene ( flaA-SVR) and high-resolution melting analysis (HRMA) of the clustered regularly interspaced short palindromic repeat (CRISPR) locus to better understand C. coli genotypic diversity and compare the suitability of these three methods for genotyping this species. Additionally, the discrimination index (DI) of each of these methods was assessed. Some C. coli strains isolated from clinical and non-clinical origins presented $80 % genotypic similarity by PFGE and flaA-SVR sequencing. HRMA of the CRISPR locus revealed only four different melting profiles. In total, 22 different flaA-SVR alleles were detected. Of these, seven alleles, comprising gt1647-gt1653, were classified as novel. The most frequent genotypes were gt30 and gt1647.This distribution reveals the diversity of selected Brazilian isolates in comparison with the alleles described in the PubMLST database. The DIs for PFGE, flaA-SVR sequencing and CRISPR-HRMA were 0.986, 0.916 and 0.550, respectively. PFGE and flaA-SVR sequencing were suitable for subtyping C. coli strains, in contrast to CRISPR-HRMA. The high genomic similarity amongst some C. coli strains confirms the hypothesis that environmental and food sources potentially lead to human and animal contamination in Brazil.

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Pulsed Field Gel Electrophoresis typing of human and retail foodstuff Campylobacters: An Irish perspective

Cited by 12 publications

References 31 publications

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis

Emerging technologies revolutionising disease diagnosis and monitoring in aquatic animal health

Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination amongst environmental, human, animal and food sources

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