Pulsed-Field Gel Electrophoretic Analysis and Some Characteristics of<i>Staphylococcus aureus</i>Isolated from Retail Foods and Human Hands

Shimamura, Yuko; Murata, Masatsune

doi:10.1271/bbb.100690

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…There was no association between PFGE patterns with the MRSA and MSSA of the 92 Thai strains which conformed to the results reported elsewhere [44, 45]. However, PFGE patterns 21 and 22 of MRSA strains predominated among isolates from Prince of Songkla Hospital and Prasat Neurological Institute, that is, 32.5 and 27.8%, respectively.…”

Section: Discussionsupporting

confidence: 89%

Staphylococcus aureusClinical Isolates: Antibiotic Susceptibility, Molecular Characteristics, and Ability to Form Biofilm

Indrawattana

Sungkhachat

Sookrung

et al. 2013

BioMed Research International

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Periodic monitoring of Staphylococcus aureus characteristics in a locality is imperative as their drug-resistant variants cause treatment problem. In this study, antibiograms, prevalence of toxin genes (sea-see, seg-ser, seu, tsst-1, eta, etb, and etd), PFGE types, accessory gene regulator (agr) groups, and ability to form biofilm of 92 S. aureus Thailand clinical isolates were investigated. They were classified into 10 drug groups: groups 1–7 (56 isolates) were methicillin resistant (MRSA) and 8–10 (36 isolates) were methicillin sensitive (MSSA). One isolate did not have any toxin gene, 4 isolates carried one toxin gene (seq), and 87 isolates had two or more toxin genes. No isolate had see, etb, or tsst-1; six isolates had eta or etd. Combined seg-sei-sem-sen-seo of the highly prevalent egc locus was 26.1%. The seb, sec, sel, seu, and eta associated significantly with MSSA; sek was more in MRSA. The sek-seq association was 52.17% while combined sed-sej was not found. Twenty-three PFGE types were revealed, no association of toxin genes with PFGE types. All four agr groups were present; agr group 1 was predominant (58.70%) but agr group 2 strains carried more toxin genes and were more frequent toxin producers. Biofilm formation was found in 72.83% of the isolates but there was no association with antibiograms. This study provides insight information on molecular and phenotypic markers of Thailand S. aureus clinical isolates which should be useful for future active surveillance that aimed to control a spread of existing antimicrobial resistant bacteria and early recognition of a newly emerged variant.

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Section: Discussionsupporting

confidence: 89%

Staphylococcus aureusClinical Isolates: Antibiotic Susceptibility, Molecular Characteristics, and Ability to Form Biofilm

Indrawattana

Sungkhachat

Sookrung

et al. 2013

BioMed Research International

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Manipulation and analysis of large DNA molecules by controlling their dynamics using micro and nanogaps

Azuma

2024

Bioscience, Biotechnology, and Biochemistry

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Manipulation and analysis methods for large DNAs are critical for epidemiological, clinical, diagnostic, and fundamental research on bacteria, membrane vesicles, plants, yeast, and human cells. However, the physical properties of large DNAs often challenge their manipulation and analysis with high accuracy and speed using the conventional methods such as gel electrophoresis and column-based methods. This review presents the approaches that leverage micrometer- and nanometer-sized gaps within microchannels to control the dynamics and conformations of large DNAs, thereby overcoming these challenges. By designing gap structures and migration conditions based on the relationship between gap parameters and the physical characteristics of large DNAs—such as diameter and persistence length—these methods enable swifter and more precise manipulation and analysis of large DNAs, including size separation, concentration, purification, and single-molecule analysis.

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Pulsed-Field Gel Electrophoretic Analysis and Some Characteristics ofStaphylococcus aureusIsolated from Retail Foods and Human Hands

Cited by 2 publications

References 29 publications

Staphylococcus aureusClinical Isolates: Antibiotic Susceptibility, Molecular Characteristics, and Ability to Form Biofilm

Staphylococcus aureusClinical Isolates: Antibiotic Susceptibility, Molecular Characteristics, and Ability to Form Biofilm

Manipulation and analysis of large DNA molecules by controlling their dynamics using micro and nanogaps

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