Pulsed lasers versus continuous light sources in capillary electrophoresis and fluorescence detection studies: Photodegradation pathways and models

Boutonnet, Audrey; Morin, Arnaud; Petit, P.; Vicendo, Patricia; Poinsot, Véréna; Couderc, François

doi:10.1016/j.aca.2016.01.036

Cited by 12 publications

(7 citation statements)

References 24 publications

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“…Different aspects of LIF detection in CE (light sources, detection elements, optimization of excitation conditions, photostability of the fluorophores, spectral filtering, and fluorescent labels) including the possibilities to further improve the LODs of on‐capillary LIF detectors were in detail analyzed in the tutorial article . Comparison of the pulsed lasers and continuous light sources have shown that continuous lasers and LEDs are more suitable for LIF detection in CE since they provide linear calibration curves, whereas pulsed lasers give polynomial ones . The nonlinearity is caused by photodegradation and photodimerization of fluorescently labeled analytes.…”

Section: Detectionmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Detectionmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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“…The photodegradation induced by pulsed UV‐LIF was investigated, explaining the non‐linear quantification using pulsed light. The main processes involved are the high photodegradation yield due to the very high pulse powers (> 800 W) and the dimerization of aromatic molecules resulting from different molecules that do or do not fluoresce .…”

Section: Detectionmentioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

et al. 2017

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Recent advances in amino acid analysis by capillary electromigration methods: June 2015-May 2017In the tenth edition of this article focused on recent advances in amino acid analysis using capillary electrophoresis, we describe the most important research articles published on this topic during the period from June 2015 to May 2017. This article follows the format of the previous articles published in Electrophoresis. The new developments in amino acid analysis with CE mainly describe improvements in CE associated with mass spectrometry. Focusing on applications, we mostly describe clinical works, although metabolomics studies are also very important. Finally, works focusing on amino acids in food and agricultural applications are also described.

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“…These UV lasers increase the selectivity of the detection because only Trp, Tyr and Phe can emit fluorescence upon 266 nm excitation. But the use of this kind of laser is problematic because the very high instant light power degrades the aromatic molecules, limiting the linearity of the detection . Moreover, it not possible to identify the non‐aromatic AA.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis/visible‐LED induced fluorescence of tryptophan: What's new?

Perquis

Ong-Meang

et al. 2019

Electrophoresis

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Tryptophane (Trp) labelled by 3‐(4‐carboxybenzoyl)‐2‐quinolinecarboxaldehyde (CBQCA) is very difficult to identify using CE and fluorescence detection (480 nm). Why in this article some mass spectrometry experiments show that Trp is really labelled by CBQCA as Leucine (Leu)? If the maximum of UV absorption (λmax) is the same between Leu‐CBQCA and Trp‐CBQCA, the molar extinction coefficient is around 2 fold higher for Trp‐CBQCA. The fluorescence of the Leu‐CBQCA derivative is 50 times more important than for Trp‐CBQCA. The addition of 7.5 mM of β‐cyclodextrin (β‐CD) was found to be a good mean to improve 2.1 fold the sensitivity of the Trp‐CBQCA fluorescence. Using a buffer containing SDS and β‐CD in CE, a LOD of 0.7 µM of L‐Trp can be reached and the ratio of the intensities between Leu, Isoleucine, Valine, Trp is 100, 21, 15, 1. Negative ESI/MS and MS/MS of the labeled amino acids show that a loss of the carboxylate function takes place. In the presence of two enantiomers of Trp‐CBQCA, we have shown that this decarboxylation is not due to the derivatization process in the solution but rather occurs in the source of the mass spectrometer.

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Pulsed lasers versus continuous light sources in capillary electrophoresis and fluorescence detection studies: Photodegradation pathways and models

Cited by 12 publications

References 24 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

Capillary electrophoresis/visible‐LED induced fluorescence of tryptophan: What's new?

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