Pulsed-Light Inactivation of Food-Related Microorganisms

Abstract

The effects of high-intensity pulsed-light emissions of high or low UV content on the survival of predetermined populations ofListeria monocytogenes, Escherichia coli,Salmonella enteritidis, Pseudomonas aeruginosa,Bacillus cereus, and Staphylococcus aureus were investigated. Bacterial cultures were seeded separately on the surface of tryptone soya-yeast extract agar and were reduced by up to 2 or 6 log10 orders with 200 light pulses (pulse duration, ∼100 ns) of low or high UV content, respectively (P < 0.001).

Peanut Allergy: Characteristics and Approaches for Mitigation

“…PUV has potential for different food processing applications (Abuagela et al, 2018;Cao, Fang, Liu, Min, & Liu, 2018;Pellicer & Gómez-López, 2017;Zhang, Wang, Zeng, Han, & Brennan, 2018). PUV is a nonthermal technology that consists of a broad spectrum of white light of wavelengths ranging from 200 nm (UV) to 1,000 nm (near-infrared; Rowan et al, 1999). The intensity of pulsed light may be up to 20,000 times more intense than sunlight (Abida, Rayees, & Masoodi, 2014).…”

Investigation of critical inter-related factors affecting the efficacy of pulsed light for inactivating clinically relevant bacterial pathogens

“…Klebsiella pneumoniae ATCC 700721 was resistant to ampicillin, ticarcillin, trimethoprim-sulfamethoxazole and gentamicin, but was susceptible to amikacin, ciprofloxacin and imipenem. The vegetative form of Bacillus cereus were prepared as per methods described previously (Rowan et al 1999). All test strains were maintained in Microbank storage vials (Cruinn Diagnostics, Dublin 12, Ireland) at )70°C.…”

“…This approach kills micro-organisms by using ultrashort-duration pulses of an intense broadband emission spectrum that is rich in UV-C germicidal light (200-280 nm band). PL is produced using techniques that multiplies power manifold by storing electricity in a capacitor over relatively long times (fractions of a second) and releasing it in a short time (millionths or thousandths of a second) using sophisticated pulse compression techniques (Rowan et al 1999;Gó mez-Ló pez et al 2007). The emitted flash has a high peak power and usually consists of wavelengths from 200 to 1100 nm broad-spectrum light enriched with shorter germicidal wavelengths (MacGregor et al 1998;Gó mez-Ló pez et al 2007).…”

“…The emitted flash has a high peak power and usually consists of wavelengths from 200 to 1100 nm broad-spectrum light enriched with shorter germicidal wavelengths (MacGregor et al 1998;Gó mez-Ló pez et al 2007). This technology has received several names in the scientific literature: pulsed UV (PUV) light (Anderson et al 2000;Sharma and Demirci 2003;Wang et al 2005), high-intensity broad-spectrum PL (Roberts and Hope 2003), PL (Rowan et al 1999), intense PL (Gó mez-Ló pez et al 2007) and pulsed white light . Seminal developments pertaining to these next generation light-flashing technologies has been the subject of recent review (Elmnasser et al 2007;Gó mez-Ló pez et al 2007), with emphasis strongly placed on decontamination efficacy for food and water applications that aptly reflects the focus of research in this field of study to date.…”

“…Significance and Impact of the Study: Findings and conclusions derived from this study will enable further development and optimization of this decontamination technique in health care and in food preparation settings, and will advance the field of nonthermal processing technologies. studies have been recently reviewed for efficacy in terms of inactivating food-related spoilage organisms and potential microbial pathogens (Elmnasser et al 2007;Gó mez-Ló pez et al 2007), and include studies carried out using lamp discharge energies of 3 J (MacGregor et al 1998;Rowan et al 1999), 7 J cm )2 Gó mez-Ló pez et al 2005), 0AE99 J cm )2 (Krishmanmurthy et al 2004, 0AE7 J cm )2 (Takeshita et al 2003) and 1 J cm )2 (Wekhof et al 2001). These studies demonstrated that factors such as number of light pulses applied, lamp discharge intensity, distance from lamp to treatment surfaces, shading, microbial species, age and density affected the efficacy of PL decontamination performances.…”