Pulsed low-intensity laser treatment stimulates wound healing without enhancing biofilm development in vitro

Besser, Manuela; Schaeler, Lukas; Plattfaut, Isabell; Brill, Florian H. H.; Kampe, Andreas; Geffken, Maria; Smeets, Ralf; Debus, Eike Sebastian

doi:10.1016/j.jphotobiol.2022.112504

Cited by 8 publications

(3 citation statements)

References 47 publications

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“…However, only a few research groups report antibacterial efficacy of LILT 34,35 . In a translational in vitro study, pulsed LILT application with a wavelength of 904nm at frequencies of 960Hz-50%, 3200Hz, and 3200Hz±50% showed no antimicrobial effect on S. aureus, P. aeruginosa, Enterococcus faecium and C. albicans in a standardised human biofilm model 36 . However, unlike the skin cells, the microorganisms were not stimulated to grow.…”

Section: Efficacy Of Antimicrobial Physical Wound Therapies Against B...mentioning

confidence: 93%

Wound biofilm: a bacterial success story

2023

JOWM

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Background Wound biofilm is one of the greatest challenges in the local therapy of chronic wounds. In clinical practice, it becomes apparent that there are significant differences in the efficacy of wound therapies, although the corresponding guidelines often classify them -due to lack of evidence and knowledge -as "equivalent".Hypothesis Since (almost) no randomised controlled clinical trials (RCT) on antimicrobial local therapies exist, translational research using human biofilm models can further generate information to demonstrate difference and equivalence of topical and physical wound therapies.Methods This narrative, but also scientific, review addresses several anti-biofilm therapies that have been validated in the translational, human biofilm model based on blood plasma, buffy coat, and various bacterial specimens. Bacterial colonisation patterns of Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) of patients and ex vivo biofilm models were compared. ResultsSubstantial differences in the anti-biofilm efficacy of antimicrobials, which were often evaluated equally in guidelines, are shown. Octenidine dihydrochloride-phenoxyethanol, polyhexanide (PHMB) and cardexomer iodine perform against biofilm with delay but well. Many antimicrobials fail because of the extrapolymeric substance (EPS) of biofilm, a bacterial product of polysaccharides and (lipo)proteins, acting like a protecting shield. Physical therapies also have their limitations here. ConclusionsThe persistence of biofilm makes wound healing stagnate. Multiple surgical debridement is not the optimal therapy for aged, critically ill patients. Wound biofilm requires a combined topical therapy because it consists of several 'components' . Currently, effective anti-biofilm strategies sustainably removing it from chronic wounds are still lacking. Researchers and manufactures are challenged, because the understanding of wound biofilm is already considerable, promising combined therapies are conceivable, only implementation is still missing.

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Section: Efficacy Of Antimicrobial Physical Wound Therapies Against B...mentioning

confidence: 93%

Wound biofilm: a bacterial success story

2023

JOWM

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“…This model takes into account the human immune system role which was absent in other in vitro models [ 48 ]. Several studies have been conducted using hpBIOM to evaluate the antibiofilm activity of conventional antimicrobial compounds and novel therapies [ 49 , 50 , 51 ].…”

Section: In Vitro Wound Modelsmentioning

confidence: 99%

Biofilms in Chronic Wound Infections: Innovative Antimicrobial Approaches Using the In Vitro Lubbock Chronic Wound Biofilm Model

Diban

Lodovico

Fermo

et al. 2023

IJMS

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Chronic wounds have harmful effects on both patients and healthcare systems. Wound chronicity is attributed to an impaired healing process due to several host and local factors that affect healing pathways. The resulting ulcers contain a wide variety of microorganisms that are mostly resistant to antimicrobials and possess the ability to form mono/poly-microbial biofilms. The search for new, effective and safe compounds to handle chronic wounds has come a long way throughout the history of medicine, which has included several studies and trials of conventional treatments. Treatments focus on fighting the microbial colonization that develops in the wound by multidrug resistant pathogens. The development of molecular medicine, especially in antibacterial agents, needs an in vitro model similar to the in vivo chronic wound environment to evaluate the efficacy of antimicrobial agents. The Lubbock chronic wound biofilm (LCWB) model is an in vitro model developed to mimic the pathogen colonization and the biofilm formation of a real chronic wound, and it is suitable to screen the antibacterial activity of innovative compounds. In this review, we focused on the characteristics of chronic wound biofilms and the contribution of the LCWB model both to the study of wound poly-microbial biofilms and as a model for novel treatment strategies.

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“…Red lasers increase cellular activity and microcirulation, stimulate immune system by the activation of different leucocyte groups, increase fibroblasts viability and improve wound healing. Infrared lasers stimulate mitochondria and blue laser has antimicrobial and anti-inflammatory effects, increase blood circulation by increasing NO-release and improving tissue supply [ 11 ]. It is not surprising that beyond the clinical symptoms of a better gingival status (color, shape, and bleeding), it was observed that mitochondria changed after laser irradiation with the activation of various metabolic pathways and an increased production of ATP [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Three Laser Wavelengths with Different Power Densities on the Mitochondrial Activity of Human Gingival Fibroblasts in Cell Culture

Nowak-Terpiłowska

Zeyland

Hryhorowicz

et al. 2023

Life

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Phototherapy plays a key role in wound healing and tissue regeneration. The use of lasers has the potential to become an effective and minimally invasive treatment in periodontal and peri-implant disease. The aim of this study was to evaluate the influence of three laser wavelengths with the combination of parameters such as power density and energy density on human gingival fibroblasts (hGFs) in vitro culture. Isolated cells were seeded in 96-well plates with culture medium (DMEM, Dulbecco’s modified Eagle’s medium) supplemented with 10% fetal bovine serum (FBS). After 24 h cells were irradiated (1064, 980 and 635 nm, various energy density value). After 24, 48 and 72 h, cells were evaluated for viability. Data were analyzed by ANOVA followed by Tukey’s HSD test. We found the best outcomes for hGFs irradiated with laser 1064 nm for all combinations of power output (50/400/1000 mW) and energy dose (3/25/64 J/cm2) after 48 h and 72 h compared with control group. Cell viability increase ranged from 0.6× (3 J/cm2, 50 mW) to 1.3× (64 J/cm2, 1000 mW). Our findings indicate that the appropriate use of low-level laser irradiation (LLLI) can increase the proliferation rate of cultured cells. The use of LLLI can be extremely useful in tissue engineering and regenerative medicine.

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Pulsed low-intensity laser treatment stimulates wound healing without enhancing biofilm development in vitro

Cited by 8 publications

References 47 publications

Wound biofilm: a bacterial success story

Wound biofilm: a bacterial success story

Biofilms in Chronic Wound Infections: Innovative Antimicrobial Approaches Using the In Vitro Lubbock Chronic Wound Biofilm Model

Influence of Three Laser Wavelengths with Different Power Densities on the Mitochondrial Activity of Human Gingival Fibroblasts in Cell Culture

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