Pulsed Ultrasound Enhances the Killing of
            <i>Escherichia coli</i>
            Biofilms by Aminoglycoside Antibiotics In Vivo

Rediske, Andrea M.; Roeder, Beverly L.; Nelson, Jared L.; Robison, Rachel L.; Schaalje, G. Bruce; Robison, Richard A.; Pitt, William G.

doi:10.1128/aac.44.3.771-772.2000

Cited by 109 publications

(95 citation statements)

References 5 publications

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“…No significant changes were incurred to the Pseudomonas aeruginosa biofilm matrix or its bacterial cells in the absence of gentamicin [88]. The viability of Escherichia coli biofilms has also been significantly reduced in vivo using pulsed low frequency ultrasound (25% duty, power intensity 500 mW/cm 2 for a treatment period of 24 or 48 h) in combination with gentamicin but these effects were not reproducible against Pseudomonas aeruginosa biofilms [89][90][91]. In 2005, Ensing et al [92] also demonstrated greater than 50% reductions in the viability of Escherichia coli biofilms by combining pulsed ultrasound treatment (24-48 kHz, power intensity 500 mW/cm 2 ) with gentamicin.…”

Section: Biofilm Prevention and Control Using Acoustic Energymentioning

confidence: 99%

Electrical methods of controlling bacterial adhesion and biofilm on device surfaces

Freebairn

Linton

Harkin‐Jones

et al. 2013

Expert Review of Medical Devices

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SummaryThis review will summarize the significant body of research within the field of electrical methods of controlling the growth of microorganisms. We examine the progress from early work using current to kill bacteria in static fluids to more realistic treatment scenarios such as flow-through systems designed to imitate the human urinary tract. Additionally, the electrical enhancement of biocide and antibiotic efficacy will be examined alongside recent innovations including the biological applications of acoustic energy systems to prevent bacterial surface adherence. Particular attention will be paid to the electrical engineering aspects of previous work, such as electrode composition, quantitative electrical parameters, and the conductive medium used. Scrutiny of published systems from an electrical engineering perspective will help to facilitate improved understanding of the methods, devices and mechanisms that have been effective in controlling bacteria, as well as providing insights and strategies to improve the performance of such systems and develop the next generation of antimicrobial bioelectric materials.

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Section: Biofilm Prevention and Control Using Acoustic Energymentioning

confidence: 99%

Electrical methods of controlling bacterial adhesion and biofilm on device surfaces

Freebairn

Linton

Harkin‐Jones

et al. 2013

Expert Review of Medical Devices

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“…Pulsed US applied for 48 hours with gentamicin was effective in eliminating E. coli infections [204], but was less effective against P. aeruginosa [205]. Vancomycin combined with US was somewhat effective against S. epidermidis infections in the same rabbit model [206].…”

Section: Antibacterialmentioning

confidence: 99%

Ultrasonic drug delivery – a general review

Pitt

Husseini

Staples

2004

Expert Opinion on Drug Delivery

557

428

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Ultrasound (US) has an ever-increasing role in the delivery of therapeutic agents including genetic material, proteins, and chemotherapeutic agents. Cavitating gas bodies such as microbubbles are the mediators through which the energy of relatively non-interactive pressure waves is concentrated to produce forces that permeabilize cell membranes and disrupt the vesicles that carry drugs. Thus the presence of microbubbles enormously enhances delivery of genetic material, proteins and smaller chemical agents. Delivery of genetic material is greatly enhanced by ultrasound in the presence of microbubbles. Attaching the DNA directly to the microbubbles or to gas-containing liposomes enhances gene uptake even further. US-enhanced gene delivery has been studied in various tissues including cardiac, vascular, skeletal muscle, tumor and even fetal tissue. US-enhanced delivery of proteins has found most application in transdermal delivery of insulin. Cavitation events reversibly disrupt the structure of the stratus corneum to allow transport of these large molecules. Other hormones and small proteins could also be delivered transdermally. Small chemotherapeutic molecules are delivered in research settings from micelles and liposomes exposed to ultrasound. Cavitation appears to play two roles: it disrupts the structure of the carrier vesicle and releases the drug; it also makes the cell membranes and capillaries more permeable to drugs. There remains a need to better understand the physics of cavitation of microbubbles and the impact that such cavitation has upon cells and drug-carrying vesicles.

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“…To this end, our research group is pursuing alternative treatments. Previous in vivo experiments showed that gentamicin combined with 24 h of ultrasonic treatment resulted in the enhanced reduction of viable bacteria in Escherichia coli biofilms (5,6). In this article, we report the successful use of ultrasound in vivo when the treatment of Escherichia coli biofilms was extended to 48 h, and the failure of such therapy to reduce viable bacteria in Pseudomonas aeruginosa biofilms.…”

Section: Introductionmentioning

confidence: 86%

“…The biofilms were grown for 24 h on polyethylene disks as reported previously (5,6). E. coli ATCC 10798 and P. aeruginosa ATCC 27853 used in these experiments had planktonic MIC values of 6 and 3 ug/mL respectively.…”

Section: Methodsmentioning

confidence: 99%

Treatment of biofilm infections on implants with low-frequency ultrasound and antibiotics

Carmen

Roeder²,

Nelson³

et al. 2005

American Journal of Infection Control

128

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SummaryMedical implants are sometimes colonized by biofilm-forming bacteria that are very difficult to treat effectively. The combination of gentamicin and ultrasonic exposure for 24 h was previously shown to reduce the viability of E. coli biofilms in vivo. This article shows that such treatment for 48 h reduced viable E. coli bacteria to nearly undetectable levels. However, when P. aeruginosa biofilms were implanted and treated for 24 and 48 h, no significant ultrasonic-enhanced reduction of viable bacteria was observed. The difference in response of these two organisms is attributed to greater impermeability and stability of the outer membrane of P. aeruginosa.

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Pulsed Ultrasound Enhances the Killing of Escherichia coli Biofilms by Aminoglycoside Antibiotics In Vivo

Cited by 109 publications

References 5 publications

Electrical methods of controlling bacterial adhesion and biofilm on device surfaces

Electrical methods of controlling bacterial adhesion and biofilm on device surfaces

Ultrasonic drug delivery – a general review

Treatment of biofilm infections on implants with low-frequency ultrasound and antibiotics

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