Pulsed versus continuous wave CO<sub>2</sub> laser excisions of 100 oral fibrous hyperplasias: A randomized controlled clinical and histopathological study

Suter, Valérie G.A.; Altermatt, Hans Jörg; Dietrich, Thomas; Warnakulasuriya, Saman; Bornstein, Michael M.

doi:10.1002/lsm.22244

Cited by 12 publications

(27 citation statements)

References 33 publications

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“…This shift was independent of the duration of the single CO 2 laser shot. Thus, the ablation of genetically encoded fluorescence in the green visible light range by a CO 2 laser occurred within the spatial range of collateral thermal damage in patients which lies on average between 150 and 160 μm …”

Section: Resultsmentioning

confidence: 99%

“…Thus, the ablation of genetically encoded fluorescence in the green visible light range by a CO 2 laser occurred within the spatial range of collateral thermal damage in patients which lies on average between 150 and 160 μm. 13,14 To analyze a fluorophore in the NIR range, spheroids were stained with the anti-CD44v6 antibody BIWA conjugated to the NIR dye IRDye800CW. Spheroids were then cut with a scalpel or burned with a CO 2 laser (Figure 2A).…”

Section: Stability Of Fluorophores After Co 2 Laser Treatmentmentioning

confidence: 99%

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Compatibility of CO₂ laser surgery and fluorescence detection in head and neck cancer cells

2018

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Background Surgical treatment of cancer requires tumor excision with emphasis on function preservation which is achieved in (early stage) laryngeal cancer by transoral carbon dioxide (CO2) laser surgery. Whereas conventional laser surgery is restricted by the surgeon's visual recognition of tumor tissue, new approaches based on fluorescence‐guided surgery (FGS) improve the detection of the tumor and its margin. However, it is unclear whether fluorophores are compatible with high‐power laser application or whether precision is compromised by laser‐induced bleaching of the dye. Methods We applied topology‐controlled 3D laser resection of fluorescent tumors cell in vitro and laser‐induced autofluorescence analysis ex vivo. Results Laser‐induced bleaching of fluorescent dyes in the visible and near‐infrared light spectrum (650‐900 nm) ranges below the resolution range of operation microscopes. Furthermore, specific fluorescent signals in an FGS mouse model is 104 higher than laser‐induced autofluorescence in mouse tissue. Conclusion Laser‐induced lateral photobleaching is negligible indicating a path forward for fluorescence‐guided laser surgery in head and neck cancer.

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Section: Resultsmentioning

confidence: 99%

Section: Stability Of Fluorophores After Co 2 Laser Treatmentmentioning

confidence: 99%

Compatibility of CO₂ laser surgery and fluorescence detection in head and neck cancer cells

2018

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“…3 The laser beam in PW has shown reduced thermal damage compared to CW in many animal studies. [25][26][27][28] In a clinical study, the thermal damage outcomes following excision biopsy of 100 fibrous hyperplasia lesions using CO 2 laser in PW and CW mode were compared. It was concluded that both laser modes produced similar thermal damage, and researchers recommended adding a 1 mm safety margin, especially in suspicious soft tissue lesions.…”

Section: Discussionmentioning

confidence: 99%

Could the super-pulsed CO2 laser be used for oral excisional biopsies?

Tenore¹,

Palaia²,

Mohsen³

et al. 2019

Adv Clin Exp Med

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Background. The main purpose of a biopsy is microscopic examination and diagnosis. Keeping the margins of specimens safe and readable is always fundamental to detecting marginal infiltrations or malignant transformation. Numerous options and tools have been introduced for biopsy procedures. Lasers are one of these options that provide many enhancements to clinical and surgical biopsy procedures in comparison to scalpels.Objectives. The aim of the present study is to quantify the thermal artefacts in histological specimens obtained using a CO 2 laser from different oral mucosal lesions and to evaluate if the resulting thermal effect hinders the histological examination. This aim is accomplished through quantitatively and qualitatively assessing the thermal effect in both the epithelium and connective tissue. Material and methods.A super-pulsed CO 2 laser (10,600 nm) was used to obtain 10 excision biopsy samples. The parameters were a power of 4.2 W in focused mode and a frequency of 80 Hz in super-pulse mode. The histological analysis was performed with an optical microscope. Computerized imaging software was utilized to quantitatively evaluate the thermal effect in both the epithelium and connective tissue expressed in microns. Results.The thermal effect of the CO 2 laser was limited to the surgical resection margins in all the specimens and did not hinder the histological analysis. Thermal artefacts were observed in 3 specimens. The range of thermal effects in the epithelial tissue was between 184 μm and 2,292 μm, while in the connective tissue it was between 133 μm and 2,958 μm. Conclusions.The resulting thermal effects of using a CO 2 laser did not hamper the histological evaluation. Utilizing a laser in biopsy procedures should be tailored. Not only should laser parameters and safety margins be taken into consideration but also the working time, clinical accessibility, and the nature and water content of the tissue.

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“…All included patients were allocated at random with a computer model to group 1, case (application of PRGF-ENDORET after surgery), or group 2, control (traditional hemostasis). 3 The local ethics committee approved the clinical protocol used for the study.…”

Section: Patient Selection and Study Designmentioning

confidence: 99%

“…Regular dental care with appropriate cleansing and disinfection measures, limitation of denture use to shorter periods, regular revision or adjustment of dentures, and if required, reconstruction of new prostheses, may prevent DIFH. 3 These lesions are very variable in shape and dimension, especially regarding the site of development and the duration of lesion evolution. It is a painless lesion.…”

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confidence: 99%

Surgical Treatment of Denture-Induced Fibrous Hyperplasia With Plasma Rich in Growth Factors

Mozzati

Mortellaro

Gallesio

et al. 2015

Journal of Craniofacial Surgery

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Denture-induced fibrous hyperplasia is a fibrous connective tissue lesion that commonly occurs in oral mucosa in patients showing important alveolar ridge atrophy. In this study, we propose Plasma Rich in Growth Factors (PRGF) to overcome constrains of traditional surgical treatment. Herein, we demonstrated that PRGF represents an autologous source of growth factors able to reduce the healing time of the alveolar mucosa and the discomfort of those patients. These properties are the result of PRGF's precise biological features that result in the following: reduction of duration and intensity of postsurgical pain, acceleration of re-epithelialization of the wound, and reduction of bleeding events and of edema. In conclusion, we showed that using PRGF on patients affected by denture-induced fibrous hyperplasia allows a short healing time, thereby reducing complications and overall improving their quality of life. The aims of this study were to evaluate the influence of PRGF-ENDORET on secondary re-epithelialization in vestibuloplasty after excision of denture irritation fibrous hyperplasia, with an explorative randomized case control trial with 10 patients, 5 patients treated with PRGF and 5 patients with traditional hemostasis, and to analyze differences with simple surgery, considering postoperative rapidity of re-epithelialization, comfort, and discomfort of patients, pain, swelling, and infections.

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Pulsed versus continuous wave CO₂ laser excisions of 100 oral fibrous hyperplasias: A randomized controlled clinical and histopathological study

Cited by 12 publications

References 33 publications

Compatibility of CO₂ laser surgery and fluorescence detection in head and neck cancer cells

Compatibility of CO₂ laser surgery and fluorescence detection in head and neck cancer cells

Could the super-pulsed CO2 laser be used for oral excisional biopsies?

Surgical Treatment of Denture-Induced Fibrous Hyperplasia With Plasma Rich in Growth Factors

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Pulsed versus continuous wave CO2 laser excisions of 100 oral fibrous hyperplasias: A randomized controlled clinical and histopathological study

Cited by 12 publications

References 33 publications

Compatibility of CO2 laser surgery and fluorescence detection in head and neck cancer cells

Compatibility of CO2 laser surgery and fluorescence detection in head and neck cancer cells

Could the super-pulsed CO2 laser be used for oral excisional biopsies?

Surgical Treatment of Denture-Induced Fibrous Hyperplasia With Plasma Rich in Growth Factors

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Product

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Pulsed versus continuous wave CO₂ laser excisions of 100 oral fibrous hyperplasias: A randomized controlled clinical and histopathological study

Compatibility of CO₂ laser surgery and fluorescence detection in head and neck cancer cells

Compatibility of CO₂ laser surgery and fluorescence detection in head and neck cancer cells