Pulvinus: an ideal explant for plant regeneration in Caesalpinia bonduc (L.) Roxb., an important ethnomedicinal woody climber

Abstract

This study demonstrates the morphogenic potential of pulvinus, an important organ situated at the base of the petiole or rachis of leguminous plants. Plant regeneration via pulvinus-derived calli of Caesalpinia bonduc has been achieved. Organogenic calli have been derived from the explant 45 days after culture on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with 6-benzylaminopurine (BA). Optimum callus induction (100%) occurred when the pulvini were cultured on MS medium fortified with 6 mg l -1 2,4-D and 1 mg l -1 BA. The highest shoot induction was obtained when the calli were transferred to MS medium supplemented with 5 mg l -1 BA and 1 mg l -1 indole-3-acetic acid (IAA). On this medium, 87% cultures responded with an average number of 4.2 shoots per culture. The maximum root induction from the regenerated shoots was observed on half strength MS medium containing 6 mg l -1 indole-3-butyric acid (IBA). Here 100% shoots rooted with a mean number of 6.3 roots per shoot. The regenerated plantlets were acclimatized and subsequently showed normal growth. This efficient protocol will be helpful for propagating elite clones on a mass scale and could be utilized for genetic transformation study.

An efficient shoot regeneration system for medicinally important Elephantopus scaber Linn.

“…At this concentration, 100% of the shoots rooted, with an average number of 3.2 roots per shoot (Table 2; Figure 2C). The use of half-strength MS medium for root induction has been reported in several systems, including Rhinacanthus nasutus (Cheruvathur and Thomas 2014) and Caesalpinia bonduc (Cheruvathur et al 2012). In the present study, the addition of IBA to the medium produced a higher response than the other auxins.…”

In Vitro Strategies for the Conservation of Some Medicinal and Horticultural Climbers

“…Pulvinus-derived callus organogenesis from C. bonduc has been reported by Cheruvathur et al ( 2012 ). Pulvinus is an organ usually seen in leguminous plants at the joint of lamina base with petiole or rachis.…”

Advancement in Encapsulation Techniques for Conservation of Climbers

“…C. bonduc is also va lued for multiple therapeutic properties like antipyretic, antidiuretic, anthelmintic, antiasthmatic, antibacterial, antiestrogenic, antiamoebic, anticonvulsant and a ntiviral activities that have been rep orted from various parts of this plant (Neogi and Nayak 1958 ;Dhar et al 1968 ;Gayaraja et al 1978 ;Adesina 1995 ). T he stu dy conducted on encapsula tion of pulvinus for micropropagation and conservation of C. bonduc by Cheruvathur et al ( 2012 ) is described as follows:…”

“…Protocol (Cheruvathur et al 2012 ) • The pulvini explants C. bonduc after following a particular washing protocol (the explants were rinsed under running water for 30 min and thereafter immersed in an aqueous solution of 5 % Teepol for 5 min, rinsed fi ve times with distilled water; then they were immersed in 70 % ethyl alcohol for 10s and rinsed with sterile distilled water three times; and fi nally they were surface disinfected with an aqueous solution of 0.1 % HgCl 2 for 5 min followed by three rinses in sterile distilled water) were cultured on MS (Murashige and Skoog 1962 ) supp lemented with 2,4-D either alone or in combination with BA for callus induction, and the highest frequency of callus induction was noticed on MS + 6 mg l −1 2,4-D.…”