Pumpless three-dimensional photo paper–based microfluidic analytical device for automatic detection of thioredoxin-1 using enzyme-linked immunosorbent assay

Lee, Myeong-Jun; Soum, Veasna; Lee, Sang-Nam; Choi, Jin‐Ha; Shin, Jeong-Hyeop; Shin, Kwanwoo; Oh, Byung-Keun

doi:10.1007/s00216-021-03747-0

Cited by 16 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Validation studies using ELISA revealed significant differences for this new biomarker between healthy and sick people. As a result, it is clear that the 3D μPAD created in the real-world example is a commercially extensible platform [ 116 ].…”

Section: Application Of μPads In the Detection Of Cancer Biomarkersmentioning

confidence: 99%

Biomarker Detection in Early Diagnosis of Cancer: Recent Achievements in Point-of-Care Devices Based on Paper Microfluidics

et al. 2023

View full text Add to dashboard Cite

Microfluidics is very crucial in lab-on-a-chip systems for carrying out operations in a large-scale laboratory environment on a single chip. Microfluidic systems are miniaturized devices in which the fluid behavior and control can be manipulated on a small platform, with surface forces on the platform being greater than volumetric forces depending on the test method used. In recent years, paper-based microfluidic analytical devices (μPADs) have been developed to be used in point-of-care (POC) technologies. μPADs have numerous advantages, including ease of use, low cost, capillary action liquid transfer without the need for power, the ability to store reagents in active form in the fiber network, and the capability to perform multiple tests using various measurement techniques. These benefits are critical in the advancement of paper-based microfluidics in the fields of disease diagnosis, drug application, and environment and food safety. Cancer is one of the most critical diseases for early detection all around the world. Detecting cancer-specific biomarkers provides significant data for both early diagnosis and controlling the disease progression. μPADs for cancer biomarker detection hold great promise for improving cure rates, quality of life, and minimizing treatment costs. Although various types of bioanalytical platforms are available for the detection of cancer biomarkers, there are limited studies and critical reviews on paper-based microfluidic platforms in the literature. Hence, this article aims to draw attention to these gaps in the literature as well as the features that future platforms should have.

show abstract

Section: Application Of μPads In the Detection Of Cancer Biomarkersmentioning

confidence: 99%

Biomarker Detection in Early Diagnosis of Cancer: Recent Achievements in Point-of-Care Devices Based on Paper Microfluidics

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Generally, there are two main types of active valves in centrifugal microfluidics: sacrificial and reversible valves. − The activation of various sacrificial valves in the LOAD occurs through the application of heat to the valves. This heating mechanism triggers the functionality of different types of sacrificial valves, such as laser-irradiated ferrowax microvalves, carbon-printed dots, and vacuum compression valves. − These valves are typically designed for single use, requiring the pre-embedding of sacrificial materials into the chip for each use .…”

Section: Introductionmentioning

confidence: 99%

On-Line Dual-Active Valves Based Centrifugal Microfluidic Chip for Fully Automated Point-of-Care Immunoassay

Qian,

Wan,

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

There remains an unmet need for a fully integrated microfluidic platform that can automatically perform multistep and multireagent immunoassays. Here, we proposed a novel online dual-active valve-based centrifugal microfluidic chip, termed DAVM, for fully automatic point-of-care immunoassay. Practically, the puncture valve, one of the dual active valves, is capable of achieving precise, on-demand, sequential release of prestored reagents, while the other valve-reversible active valve enables controlled retention and drainage of the reaction solutions. Thereby, our technology mitigates the challenges of hydrophilic/ hydrophobic modifications and unstable valve control performance commonly observed in passive valve controls. As a proof of concept, the indirect enzymatic immunoblotting technique was employed on DAVM for fully automated immunological analysis of eight targets, yielding outcomes within an hour. Furthermore, we conducted a comparative analysis of 28 clinical samples with autoimmune diseases. According to 224 clinical data, the sample testing concordance rate between DAVM and the traditional instrument was 82%, with a target compliance rate of 97%. Therefore, our DAVM system has powerful potential for fully automated immunoassays.

show abstract

“…Nitrocellulose (NC)-based paper is the other common type of substrate material that has been utilized primarily employed for LFAs [7,17,19]. In addition, applications of other paper materials, such as photo paper [20,21] and fiberglass [22], have also been explored. Many efforts have been also oriented towards the accomplishment of reliable paper-based analytical tools, especially in accuracy and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Extended functionalities of paper-based analytical devices using hydrogels for biomedical applications

Mahardika,

Kin,

Kwon

et al. 2023

Flex. Print. Electron.

Self Cite

View full text Add to dashboard Cite

Paper-based analytical devices are a strong candidate for development due to the global need for accurate, easy-to-use, and cost-effective tools. Paper offers potential as a substrate for biomedical diagnostic devices, but on its own it is limited in versatility. By combining paper with hydrogel, researchers are able to improve automation, sensitivity, affordability, flexibility, and speed. Hydrogel, a highly biocompatible material, enhances fluid flow control and the biocompatibility of paper for functional interaction with biomolecules. Hydrogel-coated paper has been utilized for various applications, including separation and detection, microfluidics, and cell culture. Here we summarize the paper-based analytical tools with hydrogel incorporated into the paper substrate for biomedical purposes. The use of hydrogel-coated paper offers new opportunities for advanced analytical tools with improved sensitivity and functionality.

show abstract

Pumpless three-dimensional photo paper–based microfluidic analytical device for automatic detection of thioredoxin-1 using enzyme-linked immunosorbent assay

Cited by 16 publications

References 31 publications

Biomarker Detection in Early Diagnosis of Cancer: Recent Achievements in Point-of-Care Devices Based on Paper Microfluidics

Biomarker Detection in Early Diagnosis of Cancer: Recent Achievements in Point-of-Care Devices Based on Paper Microfluidics

On-Line Dual-Active Valves Based Centrifugal Microfluidic Chip for Fully Automated Point-of-Care Immunoassay

Extended functionalities of paper-based analytical devices using hydrogels for biomedical applications

Contact Info

Product

Resources

About