Punch grafting of chronic ulcers in patients with laminin-332–deficient, non-Herlitz junctional epidermolysis bullosa

Yuen, Wing Yan; Huizinga, Janneke; Jonkman, Marcel F.

doi:10.1016/j.jaad.2012.04.012

Cited by 13 publications

(9 citation statements)

References 29 publications

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“…Punch grafting is a simple and frequently used technique, which has been widely employed for treating chronic non‐healing ulcers . The application of small punch grafts accelerates the healing of chronic wounds.…”

Section: Discussionmentioning

confidence: 99%

“…The application of small punch grafts accelerates the healing of chronic wounds. Multiple punch grafts, which consist of epidermis and a superficial dermal layer, cover the defect and multiply the circumference of wound edges from which keratinocytes can migrate . We can also expect a strong cytokine response and growth factor expression after grafting .…”

Section: Discussionmentioning

confidence: 99%

“…Punch grafting is a simple and frequently used technique, which has been widely employed for treating chronic non-healing ulcers. 8,9,12 The application of small punch grafts accelerates the healing of chronic wounds. Multiple punch grafts, which consist of epidermis and a superficial dermal layer, cover the defect and multiply the circumference of wound edges from which keratinocytes can migrate.…”

Section: Discussionmentioning

confidence: 99%

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Reconstruction of large wounds using a combination of negative pressure wound therapy and punch grafting after excision of acral lentiginous melanoma on the foot

Seo

Kim

Nam

et al. 2015

The Journal of Dermatology

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Melanoma in darker-pigmented individuals often develops in an acral lentiginous fashion on the foot. After surgical removal of a tumor at this site, repair of the wound can be challenging. This is because there is an insufficient local skin pool and lack of mobility of the skin in this area. Moreover, functional aspects such as walking and weight bearing should be considered. We performed a combination treatment of negative pressure wound therapy (NPWT) and punch grafting on 15 patients, after wide excision of acral lentiginous melanomas on the foot, and compared these to 26 patients who underwent either secondary intention healing (SIH, n = 13) or NPWT (n = 13) alone. The punch grafting with NPWT group showed significantly shorter healing times than those of the other two groups. Evaluation of completely healed wounds using the Vancouver Burn Scar Assessment Scale revealed that the punch grafting group had mean values better, or comparable, to the SIH or NPWT group in four of the five scales (except pigmentation). As for complications, only one patient developed a wound infection after punch grafting. Further, by utilizing NPWT for fixation of punch grafts, it was possible to treat all subjects as outpatients after punch grafting. These results show that a combination treatment of NPWT and punch grafting is an excellent therapeutic option for post-wide excision wounds on the feet, with significantly shortened healing times and favorable cosmetic outcomes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Reconstruction of large wounds using a combination of negative pressure wound therapy and punch grafting after excision of acral lentiginous melanoma on the foot

Seo

Kim

Nam

et al. 2015

The Journal of Dermatology

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“…In situ correction of LAMB3 gene in keratinocytes [ Therapeutic RNA trans-splicing molecule containing wild-type exons 1-7 [25] but punch grafting allowed only a limited expansion of the revertant skin [53]. However, expansion of the treated area can be achieved by generation of iPSCs from revertant keratinocytes, and these cells can then be differentiated into genetically corrected keratinocytes [45].…”

Section: Jeb/lamb3mentioning

confidence: 99%

Molecular Therapeutics in Development for Epidermolysis Bullosa: Update 2020

2020

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Epidermolysis bullosa (EB) is a group of rare genetic disorders for which significant progress has been achieved in the development of molecular therapies in the last few decades. Such therapies require knowledge of mutant genes and specific mutations, some of them being allele specific. A relatively large number of clinical trials are ongoing and ascertaining the clinical efficacy of gene, protein or cell therapies or of repurposed drugs, mainly in recessive dystrophic EB. It is expected that some new drugs may emerge in the near future and that combinations of different approaches may result in improved treatment outcomes for individuals with EB. Key PointsRemarkable progress has been made in understanding the molecular genetics and underlying pathomechanisms of epidermolysis bullosa (EB) forming the platform for development of treatments.Gene-replacement approaches, particularly delivery of COL7A1 to the skin of patients with severe dystrophic EB, type VII collagen replacement, skipping of exons and read-through of premature termination codons are currently in clinical trials.Preclinical research explores the applicability of new strategies in regenerative medicine (e.g., induced pluripotent stem cells) and genome editing (e.g., CRISPR/ Cas9).Particular effort is focused on severe dystrophic EB, characterized by extensive scarring and aggressive squamous cell carcinomas. Small molecules repurposed to reduce fibrosis, and the multikinase inhibitor rigosertib-for the treatment of recessive dystrophic EB squamous cell carcinomas-are being tested in clinical trials.

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“…This phenomenon is referred to as revertant mosaicism or "natural gene therapy" (104) and a key goal has been to try to exploit these natural events in the treatment of RDEB. Thus far, revertant mosaicism has not been explored therapeutically in RDEB although in some forms of junctional EB, grafting of cultured revertant keratinocytes (105) or punch grafting of revertant skin has been undertaken, with sustained improvement in recipient mutant skin sites being demonstrated for the latter (106).…”

Section: Grafting Revertant Mosaicism Skin/keratinocytesmentioning

confidence: 99%

Novel and emerging therapies in the treatment of recessive dystrophic epidermolysis bullosa

Rashidghamat

McGrath

2017

IRDR

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Punch grafting of chronic ulcers in patients with laminin-332–deficient, non-Herlitz junctional epidermolysis bullosa

Cited by 13 publications

References 29 publications

Reconstruction of large wounds using a combination of negative pressure wound therapy and punch grafting after excision of acral lentiginous melanoma on the foot

Reconstruction of large wounds using a combination of negative pressure wound therapy and punch grafting after excision of acral lentiginous melanoma on the foot

Molecular Therapeutics in Development for Epidermolysis Bullosa: Update 2020

Novel and emerging therapies in the treatment of recessive dystrophic epidermolysis bullosa

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