Puncture of a Lumbar Intervertebral Disc Induces Changes in Spontaneous Pain Behavior

Olmarker, Kjell

doi:10.1097/brs.0b013e31816b46ca

Cited by 69 publications

(74 citation statements)

References 45 publications

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“…However, surgical injury is a simple and technically easy method of inducing intervertebral disc degeneration. This method is frequently used in many studies [20,26] and can lead to intervertebral disc degeneration [25]. Mice have been used as models in the study of intervertebral disc degeneration, in particular the mechanics of the intervertebral disc in relation to human intervertebral disc degeneration [27,28].…”

Section: Discussionmentioning

confidence: 99%

Heme oxygenase-1 modulates degeneration of the intervertebral disc after puncture in Bach 1 deficient mice

et al. 2012

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Purpose Intervertebral disc degeneration is considered to be a major feature of low back pain. Furthermore, oxidative stress has been shown to be an important factor in degenerative diseases such as osteoarthritis and is considered a cause of intervertebral disc degeneration. The purpose of this study was to clarify the correlation between oxidative stress and intervertebral disc degeneration using Broad complexTramtrack-Bric-a-brac and cap'n'collar homology 1 deficient (Bach 1-/-) mice which highly express heme oxygenase-1 (HO-1). HO-1 protects cells from oxidative stress. Methods Caudal discs of 12-week-old and 1-year-old mice were evaluated as age-related models. Each group and period, 5 mice (a total of 20 mice, a total of 20 discs) were evaluated as age-related model. C9-C10 caudal discs in 12-week-old Bach 1-/-and wild-type mice were punctured using a 29-gauge needle as annulus puncture model. Each group and period, 5 mice (a total of 60 mice, a total of 60 discs) were evaluated. The progress of disc degeneration was evaluated at pre-puncture, 1, 2, 4, 8 and 12 weeks post-puncture. Radiographic, histologic and immunohistologic analysis were performed to compare between Bach 1-/-and wild-type mice. Results In the age-related model, there were no significant differences between Bach 1-/-and wild-type mice radiologically and histologically. However, in the annulus puncture model, histological scoring revealed significant difference at 8 and 12 weeks post-puncture. The number of HO-1 positive cells was significantly greater in Bach 1-/-mice at every period. The apoptosis rate was significantly lower at 1 and 2 weeks post-puncture in Bach 1-/-mice. Conclusions Oxidative stress prevention may avoid the degenerative process of the intervertebral disc after puncture, reducing the number of apoptosis cells. High HO-1 expression may also inhibit oxidative stress and delay the process of intervertebral disc degeneration.

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Section: Discussionmentioning

confidence: 99%

Heme oxygenase-1 modulates degeneration of the intervertebral disc after puncture in Bach 1 deficient mice

et al. 2012

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“…Puncture of the L4 to L5 discs resulted in increases in defined pain behaviors, including "grooming" and "wet-dog shakes." 163,164 Consistent with the role of active factors arising from the ruptured disc, a behavioral model has been developed wherein autologous nucleus pulposus is placed onto the L5 DRG exposed by unilateral facetectomy. This treatment resulted in marked ambulatory asymmetry and a preference to bear weight on the contralateral limb at extended intervals after treatment.…”

Section: Disc Injurymentioning

confidence: 99%

Behavioral Models of Pain States Evoked by Physical Injury to the Peripheral Nerve

Sorkin

Yaksh

2009

Neurotherapeutics

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Summary:Physical injury or compression of the root, dorsal root ganglion, or peripheral sensory axon leads to welldefined changes in biology and function. Behaviorally, humans report ongoing painful dysesthesias and aberrations in function, such that an otherwise innocuous stimulus will yield a pain report. These behavioral reports are believed to reflect the underlying changes in nerve function after injury, wherein increased spontaneous activity arises from the neuroma and dorsal root ganglion and spinal changes increase the response of spinal projection neurons. These pain states are distinct from those associated with tissue injury and pose particular problems in management. To provide for developing an understanding of the underlying mechanisms of these pain states and to promote development of therapeutic agents, preclinical models involving section, compression, and constriction of the peripheral nerve or compression of the dorsal root ganglion have been developed. These models give rise to behaviors, which parallel those observed in the human after nerve injury. The present review considers these models and their application.

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“…66 Recently, Olmarker has reported that rats receiving anular puncture showed a significant increase in grooming and in wet-dog shakes. 95 Likewise, Rousseau et al quantified physical disability in rats after lumbar disc stab injury. 66 The sensitivity of these measurements to assess the efficacy of drugs or treatment should be further investigated, and the standardization of methodology will be required to compare results from different groups.…”

Section: Masuda and Lotzmentioning

confidence: 99%

New Challenges for Intervertebral Disc Treatment Using Regenerative Medicine

Masuda

Lotz

2010

Tissue Engineering Part B: Reviews

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Puncture of a Lumbar Intervertebral Disc Induces Changes in Spontaneous Pain Behavior

Abstract: Puncture of a lumbar intervertebral disc in the rat produces changes in spontaneous behavior mainly seen as increased "grooming" and "wet-dog shakes," 2 behaviors that have been suggested to indicate stress and pain.

Cited by 69 publications

References 45 publications

Heme oxygenase-1 modulates degeneration of the intervertebral disc after puncture in Bach 1 deficient mice

Heme oxygenase-1 modulates degeneration of the intervertebral disc after puncture in Bach 1 deficient mice

Behavioral Models of Pain States Evoked by Physical Injury to the Peripheral Nerve

New Challenges for Intervertebral Disc Treatment Using Regenerative Medicine

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