Puncturing of lyophilized tissue engineered vascular matrices enhances the efficiency of their recellularization

Abstract

The concept of living tissue engineered, self-repairing, autologous cardiovascular replacements, was proposed alternatively to existing synthetic/xenogeneic prostheses. Recent studies in animal models demonstrate faster in vivo recellularization after grafts pre-seeding with cells prior implantation. Pre-seeded cells hold either, the ability to differentiate directionally or attract host cells, crucial for graft integration and remodeling. It is unclear, however, how efficient the pre-loading is and how well cells withstand the flow. The study presents a systematic overview of cell loading techniques of different cardiovascular constructs, tested under static and dynamic conditions. Comparison illustrates a significantly higher efficiency of cells loading in lyophilized tissues punctured before their standard seeding. This technique may beneficially accelerate remodeling of cardiovascular grafts in further in vivo studies.

Evaluation of remodeling and regeneration of electrospun PCL/fibrin vascular grafts in vivo

“…Researchers have been attempting to improve its performance. Agnieszka et al [ 15 ] has compared different reseeding methods to define the most efficient technique enhancing recellularization of tissue engineered vascular vessels prior implantation. They found that puncturing of lyophilized tissue engineered vascular matrices could enhance the efficiency of their recellularization.…”

Vascular Patches for Arterial Reconstruction, Challenges and Advanced Technologies

“…В недавнем исследовании Ksiazek с коллегами предложили собственный подход к созданию ТИЗ [40]. Нетканный пористый материал из PGA покрывали быстро деградируемым P-4-HB, а затем заселяли фетальными ММСК овцы.…”

“…Также авторы установили, что наилучшим способом заселения данного матрикса мононуклеарными клетками является предварительная лиофилизация материала с нанесением клеточной суспензии комбинированным методом капельного и инъекционного введения. Данный подход позволил доставить клетки в центр матрикса, несмотря на плотное расположение коллагеновых волокон в децеллюляризированном материале [40].…”

In Vivo Performance of Decellularized Vascular Grafts: A Review Article

“…Recently, Książek et al proposed a “puncturing-dripping” method for re-seeding tissue-engineered vascular matrices. The results demonstrated that the highest cell-loading efficiency was observed after lyophilization followed by the puncturing-dripping technique [ 60 ]. A dynamic culture system typically involves a pulsatile perfusion bioreactor to simulate blood flow/flow rate in vivo [ 61 ].…”