Punica granatum (pomegranate) leaves extract induces apoptosis through mitochondrial intrinsic pathway and inhibits migration and invasion in non-small cell lung cancer in vitro

Li, Yali; Yang, Fangfang; Zheng, Weidong; Hu, Mingxing; Wang, Juanxiu; Ma, Sisi; Deng, Yuanle; Luo, Yi; Ye, Tinghong; Yin, Wenya

doi:10.1016/j.biopha.2016.03.023

Cited by 78 publications

(70 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dysregulation of the cell cycle is a key feature of tumor cells, thus targeting the cell cycle is an important strategy in cancer therapy. Cell cycle arrest is the main pathway involved in inhibiting tumor cell proliferation (24)(25)(26). Furthermore, the activation of cell cycle checkpoints is a general cell response following exposure to cytotoxic agents (27,28).…”

Section: Discussionmentioning

confidence: 99%

CT‑1042, a novel anticancer agent, exhibits effects by activating p53 and inhibiting survivin

Yang

Li²,

Xu³

et al. 2018

Oncol Rep

View full text Add to dashboard Cite

A novel small molecular compound, 4‑ethyl‑8‑fluoro‑hydroxy‑9‑methoxy‑11‑methyl‑1,12‑dihydro‑4H‑2‑oxa‑6,12a‑diaza‑dibenzo[b,h]fluorene‑3,13‑dione (CT‑1042) exhibits potent antitumor activity against many tumor cells in vitro. However, the effects and underlying mechanisms of CT‑1042 in non‑small cell lung cancer (NSCLC) remain unclear. The present study was designed to determine the anticancer properties and underlying molecular mechanisms of CT‑1042 in NCI‑H460 NSCLC cells. A thiazolyl blue tetrazolium bromide assay (MTT) was performed to evaluate cell viability and flow cytometry was used to analyze apoptosis, mitochondrial membrane potential (MMP) and cell cycle. Real‑time quantitative PCR and western blotting were conducted to determine relative mRNA and protein levels. A tumor xenograft experiment was performed to investigate the effects of CT‑1042 on tumor growth in vivo. CT‑1042 markedly inhibited the proliferation of twelve cancer cell lines, decreased MMP in subject cells and increased caspase‑3 activity. Cell cycle analysis indicated that CT‑1042 delayed the cell cycle progression during the G2/M phase in a dose‑dependent manner. In addition, CT‑1042 induced mitochondrial‑mediated apoptosis by activating p53 and Bax, as well as inhibiting Bcl‑2 and survivin. Finally, CT‑1042 significantly suppressed NCI‑H460 xenograft tumor growth in vivo, with low systemic toxicity. Collectively, these results revealed that CT‑1042 has significant lung anticancer properties.

show abstract

Section: Discussionmentioning

confidence: 99%

CT‑1042, a novel anticancer agent, exhibits effects by activating p53 and inhibiting survivin

Yang

Li²,

Xu³

et al. 2018

Oncol Rep

View full text Add to dashboard Cite

show abstract

“…It is used externally in the treatment of vaginal discharges, mouth sores and throat infections. The whole plant, but in particular the bark, is having antibacterial, antifungal 17 , antiviral, anticancer 18 and astringent properties. The flowers are used in the treatment of dysentery, stomach ache and cough.…”

Section: Figure 4: Fruit Of Punica Granatum Linn (Anardana)mentioning

confidence: 99%

Development, characterization and evaluation of poly-herbal ointment and Gel formulation containing Nerium Indicum Mill, Artocarpus Heterophyllus Lam, Murraya Koenigii Linn, Punica Granatum Linn

Shukla¹,

Kashaw²

2019

J. Drug Delivery Ther.

View full text Add to dashboard Cite

Objective: The objective of present study was the development, characterization and evaluation of poly-herbal ointment and gel formulations of Nerium Indicum Mill, Artocarpus Heterophyllus Lam, Murraya Koenigii Linn, Punica Granatum Linn extract. Plant derived substances and herbal medicines have recently attracted the great interest towards their versatile application. Medicinal plants are the richest source of bioactive compounds used in traditional and modern medicine. The ointment and gel formulation of plant extract has a sound approach, The poly-herbal ointment and gel formulation were evaluated for its physicochemical parameters like color, odour, pH, spreadability, extrudability, consistency, diffusibility, solubility, washability, non-irritancy and stability. Materials and methods: Extract of all four plants has been separated by the soxhlet extraction by 70% ethanol. Poly-herbal ointment and gel formulation has been prepared by mixing the extract of Nerium Indicum Mill, Artocarpus Heterophyllus Lam, Murraya Koenigii Linn, Punica Granatum Linn with carbapol 940 for Gel formulation and PEG 2000 & PEG 600 for ointment formulation. Results and conclusion: Physicochemical evaluation shows that the poly-herbal ointment and gel formulations fulfilled the criteria as herbal formulation and irritancy test also performed to indicate the effectiveness and efficacy of prepared ointment and gel formulations. The poly-herbal ointment and gel formulations were also evaluated for its stability at various temperature conditions which shows no change in the irritancy, spreadability and diffusion study. Thus it could become a medium to use the medicinal properties of extracts effectively and easily as a simple dosage form. Keywords: Nerium Indicum Mill, Artocarpus Heterophyllus Lam, Murraya Koenigii Linn, Punica Granatum Linn, extract, ointment, gel.

show abstract

“…It is used externally in the treatment of vaginal discharges, mouth sores and throat infections. The whole plant, but in particular the bark, is having antibacterial, antifungal 20 , antiviral, anticancer 21 and astringent properties. The flowers are used in the treatment of dysentery, stomach ache and cough.…”

Section: Nerium Indicum Millmentioning

confidence: 99%

Extraction and wound healing potential of Nerium Indicum M, Artocarpus Heterophyllus Lam, Murraya Koenigii L, Punica Granatum L on albino rats using burn wound model

Shukla

Kashaw

2019

J. Drug Delivery Ther.

View full text Add to dashboard Cite

Objective: The objective of present work was to extract the active constituents and evaluate the wound healing potential of Nerium indicum, Artocarpus heterophyllus, Murraya koenigii, Punica granatum on albino rats using burn wound model. Poly-herbal ointment and gel formulation was developed and compare with the extract of all plants. These plants has shown anti-asthmatic, anti-diabetic, anti-inflammatory, anti-microbial, anti-leukemic, anti-mycobacterial, antibacterial, antioxidant, antiulcer, antiviral, anticancer, antimalarial, anti-inflammatory and wound healing properties. Materials and Methods: Solvent extraction method has been utilized for the separation of the active constituents of interest by using 70% ethanol as a solvent. Poly-herbal ointment and gel formulations has been prepared by mixing the extract of Nerium Indicum, Artocarpus heterophyllus, Murraya koenigii and Punica granatum in different ratio with the ointment base (10-15%) contains PEG and for gel formulation Carbapol-940 base (10-15%). Burn wound model of albino rats has been utilized for the assessment of wound healing potential of the plant extract as well as developed formulations. Results and Conclusion: The studies on burn wound healing model reveals that F2 poly-herbal ointment formulation show better burn wound healing potential in comparison to all other formulations, F-1 ointment formulation as well as extracts of all plants. There was no mortality observed in the tenure of study. Discussion: These data has indicated that ointment and gel poly-herbal formulation of Nerium Indicum, Artocarpus Heterophyllus, Murraya Koenigii and Punica Granatum has been utilized for wound healing purpose and it is safer for topical application. No toxicity and mortality has been observed during the experimental tenure. Keywords: Wound healing, Nerium indicum, Murraya koenigii, Punica granatum, Artocarpus heterophyllus, burn wound model.

show abstract

Punica granatum (pomegranate) leaves extract induces apoptosis through mitochondrial intrinsic pathway and inhibits migration and invasion in non-small cell lung cancer in vitro

Cited by 78 publications

References 44 publications

CT‑1042, a novel anticancer agent, exhibits effects by activating p53 and inhibiting survivin

CT‑1042, a novel anticancer agent, exhibits effects by activating p53 and inhibiting survivin

Development, characterization and evaluation of poly-herbal ointment and Gel formulation containing Nerium Indicum Mill, Artocarpus Heterophyllus Lam, Murraya Koenigii Linn, Punica Granatum Linn

Extraction and wound healing potential of Nerium Indicum M, Artocarpus Heterophyllus Lam, Murraya Koenigii L, Punica Granatum L on albino rats using burn wound model

Contact Info

Product

Resources

About