Purification and activity evaluation of novel anti-inflammatory peptides from pearl oyster (<i>Pinctada martensii</i>) hydrolysates

Shen, Juan; Zeng, Manjia; Chen, Bingbing; Xia, Zhen; Huang, Pantian; Cao, Yue; Miao, Jun

doi:10.1039/d2fo04046h

Cited by 9 publications

(2 citation statements)

References 39 publications

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“…However, it is essential not only to emphasize the cell surface glycan-dependent cytotoxic mechanism, as reported for the anticancer effects of the bivalve β-trefoil lectins MytiLec-1 and SeviL, but also to investigate whether lectin interactions with cell surface proteins are relevant for comprehending the cell regulatory system they control. Mitogenic activities similar to those observed for SeviL have been previously reported for other lectins, such as those from the bivalve Tridacna maxima and the gastropod Belamyia bengalensis [ 27 , 38 , 39 ]. Recently, peptides obtained by degrading proteins from pearl-producing and edible bivalves have been found to regulate the function of RAW264.7 cells [ 40 ].…”

Section: Discussionsupporting

confidence: 80%

Multifunctional Cell Regulation Activities of the Mussel Lectin SeviL: Induction of Macrophage Polarization toward the M1 Functional Phenotype

Fujii,

Kamata,

Gerdol

et al. 2024

Marine Drugs

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SeviL, a galactoside-binding lectin previously isolated from the mussel Mytilisepta virgata, was demonstrated to trigger apoptosis in HeLa ovarian cancer cells. Here, we show that this lectin can promote the polarization of macrophage cell lines toward an M1 functional phenotype at low concentrations. The administration of SeviL to monocyte and basophil cell lines reduced their growth in a dose-dependent manner. However, low lectin concentrations induced proliferation in the RAW264.7 macrophage cell line, which was supported by the significant up-regulation of TOM22, a component of the mitochondrial outer membrane. Furthermore, the morphology of lectin-treated macrophage cells markedly changed, shifting from a spherical to an elongated shape. The ability of SeviL to induce the polarization of RAW264.7 cells to M1 macrophages at low concentrations is supported by the secretion of proinflammatory cytokines and chemokines, as well as by the enhancement in the expression of IL-6- and TNF-α-encoding mRNAs, both of which encode inflammatory molecular markers. Moreover, we also observed a number of accessory molecular alterations, such as the activation of MAP kinases and the JAK/STAT pathway and the phosphorylation of platelet-derived growth factor receptor-α, which altogether support the functional reprogramming of RAW264.7 following SeviL treatment. These results indicate that this mussel β-trefoil lectin has a concentration-dependent multifunctional role in regulating cell proliferation, phenotype, and death in macrophages, suggesting its possible involvement in regulating hemocyte activity in vivo.

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Section: Discussionsupporting

confidence: 80%

Multifunctional Cell Regulation Activities of the Mussel Lectin SeviL: Induction of Macrophage Polarization toward the M1 Functional Phenotype

Fujii,

Kamata,

Gerdol

et al. 2024

Marine Drugs

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“…Pinctada martensii (Dunker) is a species of the Pteriidae, Pterioida, Bivalvia, Mollusca, and its flesh has attracted much attention because it is rich in high-quality protein. Pinctada martensii has been used in China for more than 1000 years as a natural resource for both medicine and food [ 1 ]. Pinctada martensii flesh enzymatic peptides are enzymatic peptides obtained by Pinctada martensii through acid protease hydrolysis.…”

Section: Introductionmentioning

confidence: 99%

Pinctada martensii Hydrolysate Modulates the Brain Neuropeptidome and Proteome in Diabetic (db/db) Mice via the Gut–Brain Axis

Li,

Lv,

Wei

et al. 2024

Marine Drugs

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Pinctada martensii hydrolysate (PMH) has been proved to have the effect of ameliorating disorders of glucose and lipid metabolism in db/db mice, but the mechanism of its hyperglycemia effect is still unclear. Bacterial communities in fecal samples from a normal control group, a diabetic control group, and a PMH-treated diabetes mellitus type 2 (T2DM) group were analyzed by 16S gene sequencing. Nano LC-MS/MS was used to analyze mice neuropeptides and proteomes. The 16S rDNA sequencing results showed that PMH modulated the structure and composition of the gut microbiota and improved the structure and composition of Firmicutes and Bacteroidetes at the phylum level and Desulfovibrionaceae and Erysipelatoclostridiaceae at the family level. Furthermore, the expressions of functional proteins of the central nervous system, immune response-related protein, and proteins related to fatty acid oxidation in the brain disrupted by an abnormal diet were recovered by PMH. PMH regulates the brain neuropeptidome and proteome and further regulates blood glucose in diabetic mice through the gut–brain axis. PMH may be used as a prebiotic agent to attenuate T2DM, and target-specific microbial species may have unique therapeutic promise for metabolic diseases.

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Recent progress of food‐derived bioactive peptides: Extraction, purification, function, and encapsulation

Jiang,

Sun,

Chandrapala

et al. 2024

Food Frontiers

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Functional peptides constitute a class of small molecular peptide chains with specific functions in biology and are typically composed of various amino acids. The chemical‐synthesis methods for preparation of functional peptides can bring high toxicity to the human body. Therefore, there is a growing need to explore alternative, safter sources to obtain bioactive peptides. Food‐derived bioactive peptides (FBPs) stand out as an ideal substitution offering safety and accessibility that can be used in health products and pharmaceuticals to elicit their effects. Presently, the extraction, purification, functional properties, and bioavailability of FBPs have been poorly summarized. This review aims to address this gap by summarizing key aspects of FBPs, covering their source, methods of preparation, extraction, isolation, purification, and identification. Additionally, the review explores the functional characteristics and mechanism underlying FBPs. Emphasis is placed on strategies to enhance the stability and bioaccessibility of FBPs, crucial for their successful application in the food and medical industries. Existing research findings suggest that adopting appropriate methods can extract FBPs with high yield and purity. FBPs exhibit both in vitro and in vivo biological activities regulating relevant pathways, showcasing their potential in the medical field. In the quest for improved stability, the application of nanomaterials emerges as a promising strategy. These advancements collectively hint at a bright future for FBPs in both food and medical matrices. As the field progresses, further exploration and refinement of extraction techniques, functional properties, and bioavailability will contribute to unlocking the full potential of FBPs in various applications.

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Purification and activity evaluation of novel anti-inflammatory peptides from pearl oyster (Pinctada martensii) hydrolysates

Abstract: Pearl oyster meat, a by-product of pearl production, is rich in protein, but has a low utilization rate. Our previous study showed that pearl oyster meat hydrolysates has potential anti-inflammatory...

Cited by 9 publications

References 39 publications

Multifunctional Cell Regulation Activities of the Mussel Lectin SeviL: Induction of Macrophage Polarization toward the M1 Functional Phenotype

Multifunctional Cell Regulation Activities of the Mussel Lectin SeviL: Induction of Macrophage Polarization toward the M1 Functional Phenotype

Pinctada martensii Hydrolysate Modulates the Brain Neuropeptidome and Proteome in Diabetic (db/db) Mice via the Gut–Brain Axis

Recent progress of food‐derived bioactive peptides: Extraction, purification, function, and encapsulation

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