Purification and anticancer activity of glutaminase and urease free intracellular l-asparaginase from Chaetomium sp.

Nagarajan, Arumugam; Thangavelu, Perarasu

doi:10.1016/j.pep.2021.106006

Cited by 15 publications

(5 citation statements)

References 30 publications

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“…Arumugam and Thangavelu (2022) required the production and purification of an intracellular ASNase from Chaetomium sp., where the results of the purification factor (Pf = 2.45), using two steps to purify the enzyme, were similar to those presented by this work [29].…”

Section: Discussionsupporting

confidence: 86%

“…In a systematic review on the production of l -asparaginase published by Souza et al, it was demonstrated that the time required for fungal cultivation in submerged fermentation was, on average, 4 days. From the data obtained in the growth curve above, it can be seen that 24 h was necessary for the maximum production of l -asparaginase from F. proliferatum in P. pastoris [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…Some parameters have yet to be evaluated, but it is known that enzymes produced by eukaryotic organisms are less immunogenic due to post-translational modifications. In a work published by Arumugam and Thangavelu, it took 72 h to observe the decrease in the cell viability of the MOLT-4-lineage cells at concentrations greater than 13.04 µM [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

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l-Asparaginase Type II from Fusarium proliferatum: Heterologous Expression and In Silico Analysis

Cardoso,

Souza,

Rodrigues

et al. 2023

Pharmaceutics

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The search for new drug-producing microorganisms is one of the most promising situations in current world scientific scenarios. The use of molecular biology as well as the cloning of protein and compound genes is already well established as the gold standard method of increasing productivity. Aiming at this increase in productivity, this work aims at the cloning, purification and in silico analysis of l-asparaginase from Fusarium proliferatum in Komagataella phaffii (Pichia pastoris) protein expression systems. The l-asparaginase gene (NCBI OQ439985) has been cloned into Pichia pastoris strains. Enzyme production was analyzed via the quantification of aspartic B-hydroxamate, followed by purification on a DEAE FF ion exchange column. The in silico analysis was proposed based on the combined use of various technological tools. The enzymatic activity found intracellularly was 2.84 IU/g. A purification factor of 1.18 was observed. The in silico analysis revealed the position of five important amino acid residues for enzymatic activity, and likewise, it was possible to predict a monomeric structure with a C-score of 1.59. The production of the enzyme l-asparaginase from F. proliferatum in P. pastoris was demonstrated in this work, being of great importance for the analysis of new methodologies in search of the production of important drugs in therapy.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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l-Asparaginase Type II from Fusarium proliferatum: Heterologous Expression and In Silico Analysis

Cardoso,

Souza,

Rodrigues

et al. 2023

Pharmaceutics

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“…The colonies were picked and sub-cultured on fresh agar plates to obtain pure cultures. The bacterial isolates were then screened for L-asparaginase activity using the plate method 11 .…”

Section: Methodsmentioning

confidence: 99%

Extracellular L-Asparaginase Synthesis Bacillus niacin Isolation, Optimization, and Characterization from Marine Saltern Sediment Sources

Abdul Wahab,

Palaniyandi,

Wyson

et al. 2023

AJMB

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Background: Asparagine is an amino acid that can be converted into aspartic acid and ammonia by the enzyme L-asparaginase. Some forms of cancer, such Acute Lymphoblastic Leukaemia (ALL) and Non-Hodgkin Lymphoma (NHL), respond well to this enzyme when employed as a chemotherapeutic drug. The purpose of this research was to find bacteria that can manufacture the enzymes L-asparaginasein marine slattern sediment which can be employed in commercial and industrial scale production. Methods: All of the strains were identified as Bacillus niacini spp. by biochemical and molecular testing. The strain belongs to the Bacillus genus, according to nutritional, biochemical, PCR and 16srRNA sequencing data. Results: According to the findings of this research, Bacillus niacin spp. have the potential to create a substance that is helpful in a variety of medical applications. The results of this study hint to the possibility that bacteria have the ability to produce antimicrobial compounds, which have the potential to be successful in a wide variety of environments. Conclusion: Numerous opportunities may arise for researchers interested in utilizing the medical potential of enzyme-producing bacteria if they are successfully isolated and screened from aquatic and terrestrial habitats.

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“…So, urease have wide applications as anticancer agent to treat hypertension, and nitrogen metabolism of ruminants such as cattle, sheep, etc. in immunosorbent assay as vaccines against the infection of H. pylori [22][23][24][25][26] . In addition, urease has a significant role in the wine industry [27][28] .…”

Section: Introductionmentioning

confidence: 99%

Untitled

2023

IJPSR

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Marine microorganisms are a valuable source of novel enzymes with ideal characteristics because of the halophilic nature of marine bacteria. The present study aims to isolate, screen, and identify the marine bacterial strains from marine sediment samples that produce an enzyme urease with clinical and industrial applications. Marine sediment samples were collected and cultured on zobell marine agar medium. After incubation seven bacterial strains were isolated from the culture. From this, five isolates were showed urease activity on the urea agar base medium. Morphological and biochemical test of ureaseproducing organisms was done. Urease-producing bacterial strains (UR S7B) were selected on the basis of their urease test. The selected strain UR S7B was further sequenced for identification. The phylogenetic characterization and 16S rRNA of the strain UR S7B revealed that bacterial cultures belong to Bacillus sp. The morphological studies indicated that the isolate was Gram -ve, rod-shaped and motile organism. The present study concludes that marine bacteria can be a urease source with important applications.

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Purification and anticancer activity of glutaminase and urease free intracellular l-asparaginase from Chaetomium sp.

Cited by 15 publications

References 30 publications

l-Asparaginase Type II from Fusarium proliferatum: Heterologous Expression and In Silico Analysis

l-Asparaginase Type II from Fusarium proliferatum: Heterologous Expression and In Silico Analysis

Extracellular L-Asparaginase Synthesis Bacillus niacin Isolation, Optimization, and Characterization from Marine Saltern Sediment Sources

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