Purification and Biochemical Characterization of Xylanases from Bacillus Pumilus and their Potential for Hydrolysis of Polysaccharides

Poorna, C. Asha

doi:10.4172/2167-7972.1000101

Cited by 8 publications

(10 citation statements)

References 23 publications

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“…from 1.5 to 0.5. Likewise, Shanthi and Roymon [40] in their study have also shown the highest xylanase production at 50°C. In another study, Shakoori et al [67] reported a maximum xylanase production at 45 and 50°C.…”

Section: Discussionmentioning

confidence: 64%

“…It is apparent from the present study that forest soil samples are a reservoir for a large number of xylanase-producing bacteria. It is known that soil is the common source for isolating various industrially important microorganisms [40]. This is also evidenced that forest soil contains organic constituents and minerals which make it a high microbial diversity hub [41].…”

Section: Discussionmentioning

confidence: 99%

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Optimization of xylanase from Pseudomonas mohnii isolated from Simlipal Biosphere Reserve, Odisha, using response surface methodology

Paul

Nayak

Thatoi

2020

Journal of Genetic Engineering and Biotechnology

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Background Xylanase has long been recognized as a widely used industrially important enzyme. There are some bacterial species already reported to produce xylanase which have potent xylanolytic activity towards the use of this enzyme in the production of bioethanol from lignocellulosic biomass. In this view, an efficient xylanolytic bacterial strain was isolated and screened from the soil sample of Simlipal Biosphere Reserve. Enzymatic assay for the xylanase activity was evidenced from the most potent bacterial strain, and the culture condition was optimized for obtaining the maximum enzyme activity. The most potent xylanolytic strain was also identified using biochemical and molecular methods. Results Nineteen xylanolytic bacteria (SXB1-SXB19) were isolated from Simlipal forest soil samples following dilution plate technique using corn cob xylan-enriched nutrient agar medium and screened for their xylanase-producing ability. Among these isolates, SXB19 showed maximum xylanolytic potential with a halozone size of 2.5 cm as evident in the formation of prominent yellow patches surrounding its growth in xylan-enriched nutrient agar plate. In unoptimized condition, SXB19 showed the highest enzymatic activity of 22.5 IU/ml among the 19 bacterial strains. In order to optimize the culture conditions for maximizing the xylanase production, Box-Behnken design of response surface methodology (RSM) was used. Four variables such as incubation time, pH, substrate (corn cob xylan) concentration, and temperature were considered for the RSM optimization study. From the results, it is evident that in an optimized condition of incubation time 36 h, pH 6.0, xylan concentration 0.5%, and temperature 42.5 °C, the enzyme activity reached a maximum of 152 IU/ml with nearly 6.75 times increase from the unoptimised condition. Besides, xylanase production from SXB19 was considerable in the presence of xylan followed by starch, nitrogen source such as urea followed by yeast extract, and mineral ion sources such as KCl followed by MgSO4 and ZnSO4. From different biochemical tests, 16S rRNA gene sequencing, and phylogenetic analysis, the bacterial strain SXB19 was identified as Pseudomonas mohnii. Conclusion The isolation of Pseudomonas mohnii, a potent xylanolytic bacterium from Simlipal, is a new report which opens up an opportunity for industrial production of xylanase for bioethanol production and other applications. Graphical abstract

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Optimization of xylanase from Pseudomonas mohnii isolated from Simlipal Biosphere Reserve, Odisha, using response surface methodology

Paul

Nayak

Thatoi

2020

Journal of Genetic Engineering and Biotechnology

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“…Ip-C showed Km and Vmax value of 4.59 mg/ml and 13.5 μmol/min/mg respectively. These differences in the Km and Vmax values were may be due to different xylans substrate or assay conditions (Poorna, 2011 ) The value of Vmax is in favor of beech wood xylan, the small Km value in our study shows that the purified xylanases have high affinity for the substrate and this is of significance in industrial use of the enzyme. The shelf life of enzyme was also high enough which would be important for its application.…”

Section: Discussionmentioning

confidence: 66%

Production, Purification, and Characterization of Thermostable Alkaline Xylanase From Anoxybacillus kamchatkensis NASTPD13

Yadav

Maharjan

Korpole

et al. 2018

Front. Bioeng. Biotechnol.

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Anoxybacillus kamchatkensis NASTPD13 used herein as a source for thermostable alkaline xylanase were isolated from Paudwar Hot Springs, Nepal. NASTPD13 cultured at 60°C, pH 7 and in presence of inorganic (ammonium sulfate) or organic (yeast extract) nitrogen sources, produced maximum xylanase enzyme. Xylanase production in the cultures was monitored by following the ability of culture media to hydrolyze beech wood xylan producing xylooligosaccharide and xylose by thin layer chromatography (TLC). The extracellular xylanase was isolated from optimized A. kamchatkensis NASTPD13 cultures by ammonium sulfate (80%) precipitation; the enriched xylanase preparation was dialyzed and purified using Sephadex G100 column chromatography. The purified xylanaseshowed 11-fold enrichment with a specific activity of 33 U/mg and molecular weight were37 kDa based on SDS-PAGE and PAGE-Zymography. The optimum pH and temperature of purified xylanase was 9.0 and 65°C respectively retainingmore than 50% of its maximal activity over a broad range of pH (6–9) and temperature (30–65°C). With beech wood xylan, the enzyme showed Km 0.7 mg/ml and Vmax 66.64 μM/min/mg The xylanase described herein is a secretory enzyme produced in large quantities by NASTPD13 and is a novel thermostable, alkaline xylanase with potential biotechnological applications.

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“…Like the xylanase from B. pumilus and from Chromohalobacter sp. TPSV 101 who showed a molecular weight of 14 and 15 kDa, respectively [4,5]. …”

Section: Resultsmentioning

confidence: 99%

“…Family 11 is a monospecific family, and the xylanases in this family are generally characterized by a relatively low molecular weight ranging from 19 to 25 kDa, a high pI, and a β-jelly roll fold structure [3]. Recently, low molecular weight xylanases (molecular weights of 14,000 and 15,000 KDa) have been isolated and characterized [4,5]. In fact, they are advantageous as they can easily diffuse in pulp fibers, penetrate easily within the lignocelluloses systems and then cleaved the substrate efficiently [6,7].…”

Section: Introductionmentioning

confidence: 99%

Biological Activities of Xylooligosaccharides Generated from Garlic Straw Xylan by Purified Xylanase from Bacillus mojavensis UEB-FK

Kallel¹,

Driss²,

Châabouni

et al. 2014

Appl Biochem Biotechnol

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A newly isolated Bacterium strain named UEB-FK was selected from Tunisian Sahara, exhibiting the highest clear zone on agar plates containing oat spelt xylan by staining with Congo red. On the basis of 16S rDNA sequence analysis, this strain was identified as Bacillus mojavensis. This strain produced extracellular xylanase. Xylanase from the strain was purified to homogeneity and had an apparent molecular weight of 14 kDa. The K m and V max values of the purified xylanase on oat spelt xylan were 3.85 mg/mL and 250.02 U/mg, respectively. The optimum pH and temperature for the enzyme were found to be 4.0 and 50 °C, respectively, and the enzyme exhibited significant heat stability. In addition, the enzyme was found to be stable in a wide range of pH (3-9). The main hydrolysis products yielded from garlic straw-extracted xylan were xylobiose and xylotriose. The antioxidant and antibacterial activities of xylan oligosaccharide (XOS) were investigated. As regards to the in vitro antioxidant activities, the XOS showed a important DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activity (IC50 = 0.45 mg/mL) and a high β-carotene bleaching (IC50 = 2.2 mg/mL). Furthermore, XOS had a high antimicrobial activity against Klebsiella pneumoniae, Enterococcus faecalis, Bacillus thuringiensis, and Pseudomonas aeruginosa.

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Purification and Biochemical Characterization of Xylanases from Bacillus Pumilus and their Potential for Hydrolysis of Polysaccharides

Cited by 8 publications

References 23 publications

Optimization of xylanase from Pseudomonas mohnii isolated from Simlipal Biosphere Reserve, Odisha, using response surface methodology

Optimization of xylanase from Pseudomonas mohnii isolated from Simlipal Biosphere Reserve, Odisha, using response surface methodology

Production, Purification, and Characterization of Thermostable Alkaline Xylanase From Anoxybacillus kamchatkensis NASTPD13

Biological Activities of Xylooligosaccharides Generated from Garlic Straw Xylan by Purified Xylanase from Bacillus mojavensis UEB-FK

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