Purification and characterisation of antioxidant and nitric oxide inhibitory peptides from Tilapia (Oreochromis niloticus) protein hydrolysate

Abstract

Nitric oxide (NO)-inhibitory and antioxidative activities of tilapia hydrolysates were prepared using ultrasound pretreatment at 70 W for 30 and 45 min, respectively, followed by Flavourzyme hydrolysis for 1 h. Both hydrolysates were fractionated using size exclusion chromatography on Sephadex G-25 column and purified by RP-HPLC. The amino acid sequence of the most potent and purified fractions was determined using LC/MS/MS. The antioxidant peptide (KAFAVIDQDKSGFIEEDELKLFLQNFSAGARAGDSDG DGKIGVDEFAALVK, MW: 6334.49 KDa) and NO-inhibitory peptide (AFAVIDQDKSGFIEEDELK LFLQNFSAGARAGDSDGDGKIGVDEFAALVK, MW: 6309.49 Da) produced no cytotoxicity in RAW264.7 macrophage cell lines at the concentration of 100 mg mL À1 . The purified peptides at the concentration 100 lg mL À1 possessed antioxidative and NO-inhibitory activities 83.0 AE 1.1% and 40.9 AE 0.2%, respectively, which were about 100 times those of their counterpart crude hydrolysates.

Marine Fish Proteins and Peptides for Cosmeceuticals: A Review

“…Various types of methods have been used to evaluate the antioxidant activity of fish-derived peptides, including the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay, hydroxyl radical scavenging activity, Cu 2+ chelating activity, and Fe 2+ chelating activity [ 99 , 100 , 101 , 102 , 103 , 104 , 105 ].…”

“…They are currently extensively studied in the development of cosmeceutical products, including lotions, shampoos, and moisture creams. Numerous studies have reported that marine fish-derived peptides can be used as antimicrobial agents, as shown in Table 5 [ 103 , 104 , 105 , 106 ]. The enzymes used for the isolation of antimicrobial fish peptides and the microorganisms susceptible to these antimicrobial peptides were listed in Table 5 .…”

Blue Whiting Protein Hydrolysates Exhibit Antioxidant and Immunomodulatory Activities in Stimulated Murine RAW264.7 Cells

“…Fish protein hydrolysates have also demonstrated both anti-inflammatory activity [10][11][12][13][14][15] and proinflammatory activity [16,17] via modulation of nitric oxide (NO) and inflammatory cytokines such an interleukin (IL)-6 and tumour necrosis factor-alpha (TNF-α) in macrophage cell lines. The use of macrophage cells has become an increasingly popular approach to investigate the antioxidant potential of natural bioactive compounds.…”

Nutritional, biological, and structural properties of bioactive peptides from bellflower, Persian‐willow, and bitter‐orange pollens

“…Oxidation of L‐arginine to L‐citrulline is one of the proper methods for production of NO radical. Excessive production of this radical significantly increases the risk of various types of cancer, atherosclerosis, chronic inflammation, and rheumatoid arthritis (Kangsanant et al., 2015). In this study, the effects of the type of enzyme and pollen protein on NO free radical inhibition were studied (Figure 5b).…”