Purification and characterisation of exo- and endo-inulinase from Aspergillus ficuum JNSP5-06

Chen, Hanqing; Chen, Xiaoming; Li, Yin; Wang, Jing; Jin, Zhengyu; Xu, Xueming; Zhao, Jianwei; Chen, Tianxiang; Xie, Zhengjun

doi:10.1016/j.foodchem.2009.01.067

Cited by 68 publications

(42 citation statements)

References 30 publications

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“…Similar results have been reported by Paula et al (2008) and the effect of pH on free and immobilized inulinase was the same. These results were also comparable with other purified exo-inulinases reported so far such as A. ficuum JNSP5-06 (pH 4.5) (Chen et al, 2009); and A. fumigatus (pH 6.0) (Gill et al, 2006b). Kango (2008) reported similar influence of pH optimum of A. niger NK-126 inulinase at pH 5.0 and temperature optimum of 50°C.…”

Section: Optimum Ph Temperature and Thermostability Profilesupporting

confidence: 90%

“…These results are in accordance with those noticed for exo-inulinases of Aspergillus fumigatus, Aspergillus ficuum and Sphingomonas sp. JB13 (Chen et al, 2009;Gill et al, 2006a;Zhou et al, 2015).…”

Section: Effect Of Metal Ions and Inhibitorsmentioning

confidence: 99%

“…Microbial inulinases belong to two classes (i) exo-inulinase (EC 3.8.1.80; fructan β-fructosidases, exo-β-D-fructosidases) which splits the terminal fructose link from inulin to generate high fructose and (ii) endoinulinase (EC 3.2.1.7) that hydrolyzes internal linkage in inulin to liberate fructooligosaccharides (FOS) (Chen et al, 2009;Housseiny, 2014;Rawat, et al, 2015a). Inulin rich plant materials present a great opportunity for exploitation and generation of value-added products such as bio-ethanol, single cell protein, citric acid, FOS and high fructose syrup.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor

Rawat

Soni

Kango

et al. 2017

Biocatalysis and Agricultural Biotechnology

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Section: Optimum Ph Temperature and Thermostability Profilesupporting

confidence: 90%

Section: Effect Of Metal Ions and Inhibitorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor

Rawat

Soni

Kango

et al. 2017

Biocatalysis and Agricultural Biotechnology

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“…4). Chen et al [2] purified three exoinulinases (Exo-I, Exo-II, and Exo-III) and two endoinulinases (Endo-I and Endo-II) from the culture broth of A. ficuum JNSP5-06 by ammonium sulfate precipitation, DEAE-cellulose column chromatography, Sepharose CL-6B column chromatography. Extracellular inulinase was purified from A. niger by ion exchange chromatography on DEAE Sepharose with specific activity 750 U/mg protein [26].…”

Section: Resultsmentioning

confidence: 99%

Partial purification of extracellular exo-inulinase from Ulocladium atrum

El-Souod

Mohamed

Ali

et al. 2014

Journal of Genetic Engineering and Biotechnology

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Eight fungal species were cultivated on the Czapek liquid medium and a good starting extracellular and intracellular exo-inulinase were selected. Extracellular inulinase from Ulocladium atrum was prepared in the presence of 1% inulin source and 0.2% sodium nitrate as the best carbon and nitrogen sources. Incubation for the U. atrum was increased till it reached its maximum (36 U/ ml) at the sixth day of incubation at 30°C which was the best temperature for the production of exo-inulinase. Effect of all metal ions inhibited inulase production by U. atrum. Exo-inulinase was purified by using ammonium sulfate precipitation, ion exchange chromatography on DEAEcellulose. Three active inulinase forms INI, INII and INIII were resolved, each for DEAE cellulose. The specific activity of INI was 1915 U/mg protein which represented 2.65-fold purification over the crude extract with 42.8% recovery pooling of INI placed on CM cellulose chromatography and INI was resolved into INIa, INIb and INIc. The specific activity of INIa was 2479.2 U/mg protein which represented 3.43-fold purification over the crude extract with 24.2% recovery. ª 2014 Production and hosting by Elsevier B.V. on behalf

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“…fi cuum JNSP5-06 were 14.8 and 25.6 mg/mL, respectively (21). A. fi cuum JNSP5-06 endoinulinase was expressed in E. coli and the K m and v max values with inulin as the substrate were found to be (67.4±4.2) mg/mL and (349.2±13.7) mg/(mL·min), respectively (22).…”

Section: Kinetic Properties Of Inulinasementioning

confidence: 99%

Purification and Characterization of an Endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 Mutant

Naidoo

Kumar

Sharma

et al. 2015

Food Technol. Biotechnol.

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SummaryAn extracellular endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 mutant was purifi ed to homogeneity by gel fi ltration chromatography and showed a specifi c activity of 119 U/mg. The optimum pH and temperature of the purifi ed enzyme were found to be 6.0 and 50 °C, respectively. The enzyme was stable up to 60 °C, retaining 60 % of residual activity for 30 min, but inactivated rapidly above 60 °C. The enzyme was found to be stable at pH=6-9 when it retained 100 % of its residual activity. The Lineweaver-Burk plot showed that the apparent K m and v max values of the inulinase when using inulin as a substrate were 1.15 mg/mL and 0.15 μM/min, respectively, whereas the k cat value was found to be 0.145 min -1 . The calculated catalytic effi ciency of the enzyme was found to be 0.126 (mg·min)/mL. The purifi ed inulinase can be used in the production of high fructose syrups.

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Purification and characterisation of exo- and endo-inulinase from Aspergillus ficuum JNSP5-06

Cited by 68 publications

References 30 publications

Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor

Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor

Partial purification of extracellular exo-inulinase from Ulocladium atrum

Purification and Characterization of an Endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 Mutant

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