Purification and characterization a polysaccharide from Hedyotis diffusa and its apoptosis inducing activity toward human lung cancer cell line A549

Lin, Liyao; Cheng, Kun; Xie, Zhanqiang; Chen, Chun-Yuan; Lin, Chen; Huang, Yongye; Zhu, Liang

doi:10.1016/j.ijbiomac.2018.10.077

Cited by 39 publications

(20 citation statements)

References 29 publications

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“…Bax) protein [33]. Evidence indicates that an increase in Bax/Bcl-2 ratio would change the permeability of the mitochondrial membranes, which also results in the release of cytochrome c and the subsequent activation of caspases [34,35]. Among them, cleaved-caspase-3 serves as the key executioner in cell death through receptor-mediated or mitochondria dependent-induced apoptosis [36].…”

Section: Discussionmentioning

confidence: 99%

Protective effects of delphinidin against H2O2–induced oxidative injuries in human retinal pigment epithelial cells

Yang

Xing

2019

Bioscience Reports

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Age-related macular degeneration (AMD) is now one of the leading causes of blindness in the elderly population and oxidative stress-induced damage to retinal pigment epithelial (RPE) cells occurs as part of the pathogenesis of AMD. In the present study, we evaluated the protective effect of delphinidin (2-(3,4,5-trihydroxyphenyl) chromenylium-3,5,7-triol) against hydrogen peroxide (H2O2)-induced toxicity in human ARPE-19 cells and its molecular mechanism. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry demonstrated that pretreatment of ARPE-19 cells with delphinidin (25, 50, and 100 μg/ml) significantly increased cell viability and reduced the apoptosis from H2O2 (0.5 mM)-induced oxidative stress in a concentration-dependent manner, which was achieved by the inhibition of Bax, cytochrome c, and caspase-3 protein expression and enhancement of Bcl-2 protein. The same tendency was observed in ARPE-19 cells pre-treated with 15 mM of N-acetylcysteine (NAC) before the addition of H2O2. Furthermore, pre-incubation of ARPE-19 cells with delphinidin markedly inhibited the intracellular reactive oxygen species (ROS) generation and Nox1 protein expression induced by H2O2. Moreover, the decreased antioxidant enzymes activities of superoxide dismutase (SOD), catalase (CAT), and glutathione-peroxidase (GSH-PX) and elevated (MDA) level in H2O2-treated cells were reversed to the normal standard by the addition of delphinidin, which was regulated by increasing nuclear Nrf2 protein expression in ARPE-19 cells. Our results suggest that delphinidin effectively protects human ARPE-19 cells from H2O2-induced oxidative damage via anti-apoptotic and antioxidant effects.

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Section: Discussionmentioning

confidence: 99%

Protective effects of delphinidin against H2O2–induced oxidative injuries in human retinal pigment epithelial cells

Yang

Xing

2019

Bioscience Reports

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“…The anti-apoptotic gene Bcl-2 was the first anti-apoptotic gene discovered in biomedical research (39). Bax is a pro-apoptotic gene opposing the activity of apoptosis-inhibiting proteins, including Bcl-2, to prevent the anti-apoptotic effects (40).…”

Section: Discussionmentioning

confidence: 99%

Effect of miR‑140‑5p on the regulation of proliferation and apoptosis in NSCLC and its underlying mechanism

et al. 2019

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Non-small cell lung cancer (NSCLC) is the most common type of lung cancer accounting for ~80% of lung cancer cases. According to novel research, numerous microRNAs (miRs) have been suggested to function as important regulators of cancer. In addition, the expression of miR-140-5p is decreased in patients with NSCLC. Therefore, it is important to further elucidate the role of miR-140-5p in NSCLC. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used in order to investigate the expression of miR-140-5p in NSCLC tissues and matched normal tissues and to determine miR-140-5p levels following transfection with mimics into A549 lung cancer cells. Targetscan software was used to predict the oncogene target of miR-140-5p. This analysis revealed that YES proto-oncogene 1 (YES1) includes a target site for miR-140-5p binding. The results revealed that YES1 is a potential target gene of miR-140-5p, and this was further confirmed by the results of luciferase reporter assays, which demonstrated that miR-140-5p directly targeted the predicted binding site in the 3'-untranslated region of YES1. Cell Counting Kit-8 (CCK-8) and flow cytometry assays were performed to determine the levels of cell viability and apoptosis. Western blot assays was performed to investigate the expression levels of YES1 and proteins associated with apoptosis in A549 cells following transfection. The results revealed that miR-140-5p expression was significantly downregulated in NSCLC tissues compared with matched normal tissues. The expression of miR-140-5p was significantly increased following transfection with miR-140-5p mimics. The results of CCK-8 and flow cytometry assays indicated that miR-140-5p inhibited proliferation and induced apoptosis of tumor cells. Western blot analysis and RT-qPCR revealed that YES1 and B-cell lymphoma 2 (Bcl-2) mRNA and protein expression levels were markedly decreased in A549 cells, while Bcl-2 associated X (Bax) and caspase-3 expression levels increased significantly following transfection with miR-140-5p mimics compared with the negative control group. In conclusion, miR-140-5p may induce apoptosis in A549 cells by targeting YES1 and regulating the expression of apoptosis-associated proteins Bcl-2, Bax and caspase-3.

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“…Lung cancer was treated with SDP for 15 days, and all nude mice were observed to show no signs of death or toxicity. Further research found that SDP could induce A549 cells apoptosis, but had no significant effect on normal human fibroblasts WI38 (Lin et al, 2019). In addition, no mice died and no significant reduction in body weight was observed during 3 weeks of CAs-treated liver cancer, indicating that CAs have no significant toxicity to host animals (Zhao Y. et al, 2017).…”

Section: The Toxicity Analysis Of Plant Polysaccharidesmentioning

confidence: 95%

“…polysaccharide (SDP) which consists of glucose, galactose, mannose ratio of 2.0: 1.0: 1.0. High-dose SDP exhibited tumor inhibition rates comparable to cisplatinpositive drugs (Lin et al, 2019). After treatment with Citrus × aurantiifolia (Christm.)…”

Section: Apoptosis and Plant Polysaccharidesmentioning

confidence: 98%

Modulation of Apoptosis by Plant Polysaccharides for Exerting Anti-Cancer Effects: A Review

Gan

Wang

et al. 2020

Front. Pharmacol.

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Cancer has become a significant public health problem with high disease burden and mortality. At present, radiotherapy and chemotherapy are the main means of treating cancer, but they have shown serious safety problems. The severity of this problem has caused further attention and research on effective and safe cancer treatment methods. Polysaccharides are natural products with anti-cancer activity that are widely present in a lot of plants, and many studies have found that inducing apoptosis of cancer cells is one of their important mechanisms. Therefore, this article reviews the various ways in which plant polysaccharides promote apoptosis of cancer cells. The major apoptotic pathways involved include the mitochondrial pathway, the death receptor pathway, and their upstream signal transduction such as MAPK pathway, PI3K/AKT pathway, and NF-kB pathway. Moreover, the paper has also been focused on the absorption and toxicity of plant polysaccharides with reference to extant literature, making the research more scientific and comprehensive. It is hoped that this review could provide some directions for the future development of plant polysaccharides as anticancer drugs in pharmacological experiments and clinical researches.

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Purification and characterization a polysaccharide from Hedyotis diffusa and its apoptosis inducing activity toward human lung cancer cell line A549

Cited by 39 publications

References 29 publications

Protective effects of delphinidin against H2O2–induced oxidative injuries in human retinal pigment epithelial cells

Protective effects of delphinidin against H2O2–induced oxidative injuries in human retinal pigment epithelial cells

Effect of miR‑140‑5p on the regulation of proliferation and apoptosis in NSCLC and its underlying mechanism

Modulation of Apoptosis by Plant Polysaccharides for Exerting Anti-Cancer Effects: A Review

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