Purification and characterization of a highly specific polyclonal antibody against human extracellular signal-regulated kinase 8 and its detection in lung cancer

Cai, Na-Li; Lau, Andy T. Y.; Yu, Feiyuan; Wu, Dandan; Dai, Lijuan; Mo, Hai-Ying; Lin, Cheng Huang; Xu, Yan‐Ming

doi:10.1371/journal.pone.0184755

Cited by 11 publications

(7 citation statements)

References 15 publications

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“…Apart from the intracellular environment of lung tumor cells influencing the relaxivity of GdCho-Len, detection of lung tumor cells was difficult to see on the imaging volume within which these cells were embedded (43)(44)(45). The present study indicated that the GdCho-Len allowed Len to discriminate between lung cancer cells, which enhanced the diagnostic sensitivity of PET/CT.…”

Section: Discussionmentioning

confidence: 67%

Role of Gd2O3‑doped carbon‑11‑choline‑lenvatinib nanoparticles contrast agent PET/CT in the diagnosis of patients with lung cancer

et al. 2019

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Positron emission tomography-computed tomography (PET/CT) is an efficient method for the diagnosis of various types of human cancer. Studies have demonstrated that Gd 2 O 3-doped carbon-11-choline (GdCho) can be used as a contrast nanoparticle for PET/CT in the diagnosis of patients with lung cancer. The aim of the present study was to evaluate the effect of GdCho-lenvatinib nanoparticles contrast-PET/CT (GdCho-Len-PET) in the diagnosis and treatment planning of a cohort of patients suspected of having lung cancer. The results of the present study demonstrated that GdCho-Len could be used as an efficient PET/CT contrast agent for the diagnosis of patients with lung cancer. GdCho-Len nanoparticles contrast agent exhibited a significantly improved longitudinal relaxivity compared with GdCho. The outcomes of the present study were that GdCho-Len-PET diagnosed 152 patients with lung cancer, whereas GdCho-PET diagnosed 130 patients with lung cancer among the 172 patients. GdCho-Len-PET presented with higher accuracy and sensitivity compared with GdCho-PET in diagnosing patients with lung cancer. All patients were further confirmed via histological analysis. GdCho-Len-PET contributed to the anticancer treatments in 56 out of 62 (90.3%) patients with lung cancer who were candidates for radiation therapy, 52 out of 57 (91.2%) patients with lung cancer undergoing adjuvant radiotherapy, and 13 out of 17 (76.5%) patients with lung cancer undergoing comprehensive therapy. Patients diagnosed using GdCho-Len-PET improved the survival of patients with lung cancer during a 420-day follow up. In conclusion, GdCho-Len-PET increased the diagnostic efficacy and had a significant effect on survival for patients with lung cancer, and may therefore serve as a reliable method for human cancer diagnosis.

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Section: Discussionmentioning

confidence: 67%

Role of Gd2O3‑doped carbon‑11‑choline‑lenvatinib nanoparticles contrast agent PET/CT in the diagnosis of patients with lung cancer

et al. 2019

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“…Similarly, MAPK15 overexpression may contribute to the malignant transformation of male germ cells by controlling a "stress support" autophagic pathway, which prevents DNA damage and the consequent activation of the tumor suppressor TP53 (Rossi et al, 2016). Our recent tissue array analysis also demonstrated that the MAPK15 protein was localized in both the nucleus and cytoplasm of human lung cancers (Cai et al, 2017 (Groehler & Lannigan, 2010). Previously, it has been shown that loss of MAPK15 was correlated with breast cancer progression, and all estrogen receptor (ESR) alpha-positive breast tumors had decreased MAPK15 expression compared with that found in normal breast tissue (Henrich et al, 2003).…”

Section: Mapk15 Protein Interactomementioning

confidence: 69%

Regulation of human mitogen‐activated protein kinase 15 (extracellular signal‐regulated kinase 7/8) and its functions: A recent update

Lau

2018

Journal Cellular Physiology

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Mitogen-activated protein kinase 15 (MAPK15), originally also known as extracellular signal-regulated kinase 7/8, is the most recently identified atypical MAPK and the least studied so far. Examinations of the role of MAPK15 in various cell lines and model systems indicate that MAPK15 participates in a variety of cellular activities such as promoting cell proliferation, cell transformation, and apoptosis; stimulating autophagy; regulating cell division, ciliogenesis, and protein secretion; and maintaining genome stability. As multiple roles of MAPK15 were observed among these studies, therefore, it remains unclear whether MAPK15 acts as a proto-oncogene or tumor suppressor. Here, the recent literature on human MAPK15 and the resulting functions will be discussed.

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“…Their results revealed that the raised rabbit-specific pAbs against human extracellular signal-regulated kinase 8 were able to perform as a sensitive antibody for cancer case studies. (36) Considering the WB analyzing, our generated camelid pAbs were able to detect recombinant CTLA-4 protein on the nitrocellulose membrane (Fig. 4).…”

Section: Discussionmentioning

confidence: 96%

Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody Against Recombinant Cytotoxic T-Lymphocyte-Associated Protein-4

Sotoudeh

Noormohammadi

Habibi‐Anbouhi

et al. 2019

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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Cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is a critical negative immunomodulatory receptor that is normally expressed in activated T cells and noticeably, in many cancerous cells. Indeed, molecular detection of CTLA-4 protein is crucial in basic research. In this work, the extracellular domain of the human CTLA-4 (hCTLA-4) protein was cloned, expressed, and purified. Subsequently, this protein was used as an antigen for camel (Camelus dromedarius) immunization to obtain polyclonal camelid sera against this protein. Furthermore, we evaluated the benefits of applying camelid hyperimmune sera containing heavy-chain antibodies in different antibody-based techniques. Our results indicated that hCTLA-4 protein was successfully expressed in the prokaryotic system. The polyclonal antibody (pAb) that raised against recombinant hCTLA-4 protein was able to detect the CTLA-4 protein in antibody-based techniques, such as enzyme-linked immunosorbent assay, western blotting, flow cytometry and immunohistochemistry (IHC) staining. This study shows that, due to the advantages such as multi-epitope-binding ability, camelid pAbs are potent to efficiently apply for molecular detection of CTLA-4 receptors in fundamental antibody-based researches such as IHC.

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Purification and characterization of a highly specific polyclonal antibody against human extracellular signal-regulated kinase 8 and its detection in lung cancer

Cited by 11 publications

References 15 publications

Role of Gd2O3‑doped carbon‑11‑choline‑lenvatinib nanoparticles contrast agent PET/CT in the diagnosis of patients with lung cancer

Role of Gd2O3‑doped carbon‑11‑choline‑lenvatinib nanoparticles contrast agent PET/CT in the diagnosis of patients with lung cancer

Regulation of human mitogen‐activated protein kinase 15 (extracellular signal‐regulated kinase 7/8) and its functions: A recent update

Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody Against Recombinant Cytotoxic T-Lymphocyte-Associated Protein-4

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