Purification and characterization of a lipase from Pseudomonas aeruginosa KKA-5 and its role in castor oil hydrolysis

Production, purification, characterization, and applications of lipases

“…This enzyme could cause up to 90% hydrolysis of castor oil and it was stable in alkaline conditions (pH 7-10). The maximum activity was obtained at pH 8.5 (Sharon et al, 1998).…”

“…In the same yeast, the production of a constitutive lipase was induced by using glucose as the carbon source . P. aeruginosa KKA-5 produced the maximal lipase activity when castor oil (2%) was used as the carbon source at pH 6.9 (Sharon et al, 1998). This enzyme could cause up to 90% hydrolysis of castor oil and it was stable in alkaline conditions (pH 7-10).…”

“…At concentrations greater than 7%, corn steep liquor caused a rapid decline in cell growth and lipase production. P. aeruginosa KKA-5 produced an extracellular lipase in a medium composed of polypeptone (4%) and yeast extract (0.05%) (Sharon et al, 1998). This enzyme was stable up to 45°C.…”

Lipase catalyzed ester synthesis for food processing industries

“…(Ghosh et al, 1996). The salts of the heavy metals such as Fe 2+ , Zn 2+ , Hg 2+ , Fe 3+ strongly inhibited the lipase, suggesting that they were able to alter the enzyme conformation (Sharon et al, 1998).…”

“…To purify a protein to homogeneity, with an overall yield of 30% and a purification factor of 320, four or five purification steps are usually required. Nowadays, some novel purification technologies are employed, which include membrane processes, immunopurification, hydrophobic interaction chromatography employing epoxy-activated spacer arm as a ligand and polyethylene glycolsepharose gel, poly (vinyl alcohol) polymers as column chromatography stationary phase and aqueous tow-phase systems (Sharon et al, 1998) reversed miceller process (Ghosh et al, 1996).…”