Purification and characterization of a glycoprotein inhibitor of toxic phospholipase from Withania somnifera

Deepa, Mohan; Gowda, T. Veerabasappa

doi:10.1016/s0003-9861(02)00527-1

Cited by 38 publications

(22 citation statements)

References 55 publications

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“…Injection of group IIA sPLA 2 from human synovial fluid and snake venom (group IA, group IIA and IIB) into animal joints confirms the development of acute inflammatory response [24][25][26]. Demonstration of sPLA 2 inhibitors as suppressor of inflammatory process provides a strong evidence for their role in inflammation [27,28]. However, other human sPLA 2 s are currently under intensive research regarding their involvement in various biological functions and their role in diseased conditions.…”

Section: Discussionmentioning

confidence: 99%

Group IIA Secretory PLA2 Inhibition by Ursolic Acid: A Potent Anti-Inflammatory Molecule

Angaswamy

Gowda²,

Rajesh

et al. 2007

CTMC

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Ursolic acid (3beta-hydroxy-urs-12-en-28-oic acid) isolated from many medicinal plants has diverse pharmacologically important properties, including strong anti-inflammatory activity. However its interaction with pro-inflammatory PLA2 is not known. Ursolic acid inhibited secretory PLA2 (sPLA2) enzymes purified from Vipera russelli, Naja naja venom and human pleural fluid and synovial fluid. IC50 values determined for these enzymes ranged from 12 to 18 microM. Group II secretory PLA2 from both venoms & human inflammatory source were found to be sensitive to inhibition in comparison with group I cobra venom sPLA2. Variation in Ca2+ concentration from 2.5-15 mM did not alter the level of inhibition. Similarly sPLA2 inhibition by ursolic acid is independent of substrate concentration. Ursolic acid interacts with purified venom sPLA2 enzymes and enhances relative fluorescence intensity in a dose dependent manner. In the presence of ursolic acid apparent shift in the far UV-CD spectra of sPLA2 was observed, indicating a direct interaction with the enzyme and formation of enzyme-ursolic acid complex. This complex results in irreversible inhibition of sPLA2 as evident by dialysis study. Inhibition of sPLA2 induced mouse paw edema and indirect hemolytic activity confirmed its sPLA2 inhibitory activity in vivo and in situ respectively. These studies revealed that the strong anti-inflammatory activity of ursolic acid is by inhibiting sPLA2 enzymes.

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Section: Discussionmentioning

confidence: 99%

Group IIA Secretory PLA2 Inhibition by Ursolic Acid: A Potent Anti-Inflammatory Molecule

Angaswamy

Gowda²,

Rajesh

et al. 2007

CTMC

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“…Injection of inflammatory HSF PLA 2 (group IIA) and snake venom PLA 2 (group IA, group IIA and IIB) into animal joints confirms the development of acute inflammatory responses. Demonstration of sPLA 2 inhibitors as suppressor of inflammatory process provides a strong evidence for their role in inflammation (Jameel et al, 2005;Teixeira et al, 2003;Bomalaski et al, 1991;Weichman et al, 1989;Deepa and Gowda, 2002;Vishwanath et al, 1988). However, other human sPLA 2 s are under intensive research regarding their involvement in various biological functions and in diseases.…”

Section: Discussionmentioning

confidence: 99%

α-lipoic acid: An inhibitor of secretory phospholipase A2 with anti-inflammatory activity

2006

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“…These are inhibited by the aqueous leaf extract of Witlania somnifera. It is due to the glycoprotein as responsible for this activity 23 .…”

Section: Resultsmentioning

confidence: 99%

Untitled

2018

IJPSR

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Nanobiotechnology in contemporary society is novel and has enhanced utility for size, distribution and morphology determination of various biomolecules. To prepare nano particles the used metals are gold, silver, mercury and platinum for significant applications in electronics, magnetics, optoelectronics and information storage. For our study we selected the sliver, since it is widely been used from ancient time for the treatment of bacteria, microbes, infections and serious burns as medicine or cosmetics and lotions in an emergence of new therapeutic agents. Plants are considered as environment friendly and efficient to produce in large-scale synthesis. So we selected the Trichodesma indicum for our study with combination of silver nitrate for silver ingredient in synthesis of silver nanoparticles (AgNPs) and their anti venom activity was studied. The properties of AgNPs of T. indicum were submitted to another journal had spherical particles of sizes 21 -25 nm with active functional moieties of amino, carboxy, alcoholic and aromatics which contained the zeta potential of 30.9V. These properties of stem extract particles found to inhibit enzymes of cobra venom at 10 and 20 µg/ml. However the fluorescent microscopy analysis of these two concentrations has revealed that the stem extract when compared to leaf extract formation of some cell clumps were even not found. Therefore AgNPs at 10µg/ml having various shapes and sizes inhibit the venom activities of cobra in blood systems. Thus in our work the activity studies of T. indicum against the cobra venom has confirmed inhibitory effects in in vitro models. This might be due to secondary metabolites that are present in T. indicum plant, which are active and might explain the bioactivity of the extract. However more of additional in vivo inhibitory studies are required in identifying the metabolites for the requirement of human beings.

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Purification and characterization of a glycoprotein inhibitor of toxic phospholipase from Withania somnifera

Cited by 38 publications

References 55 publications

Group IIA Secretory PLA2 Inhibition by Ursolic Acid: A Potent Anti-Inflammatory Molecule

Group IIA Secretory PLA2 Inhibition by Ursolic Acid: A Potent Anti-Inflammatory Molecule

α-lipoic acid: An inhibitor of secretory phospholipase A2 with anti-inflammatory activity

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