Purification and characterization of a novel antithrombotic peptide from Scolopendra subspinipes mutilans

Kong, Yi; Huang, Shu; Shao, Yu; Li, Shuai; Wei, Ji‐Fu

doi:10.1016/j.jep.2012.10.048

Cited by 44 publications

(26 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Over the last decades, many fibrinolytic enzymes have been identified and characterised from a variety of sources, including plants [31], animals [7,9] and microorganisms [32]. Among them, medicinal animals are a major source of thrombolytic agents, such as lumbrokinase and earthworm fibrinolytic enzyme II from earthworms [8,33], Agacutase from D. acutus snake venoms [10,11], hirudins from leech Hirudinaria manillensis [6], fibrinolytic protease UFEIII from Urechis unicinctus [4], and several of these compounds, such as hirudin [34], lumbrokinase [35], ancrod [36] have been widely applied in the clinic and achieved good results.…”

Section: Discussionmentioning

confidence: 99%

“…In the study, comparing with other fibrinolytic enzymes [29,31,42], we found that PSLTro01 could also inhibit carrageenan-induced tail thrombosis and the effect was similar to that of urokinase. A lot of fibrinolytic enzymes [7,10,43] not only have antithrombotic effect but also have anticoagulant activity. The blood coagulation cascade includes the intrinsic blood coagulation pathway, extrinsic blood coagulation pathway, and common blood coagulation pathway.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Purification and biochemical characterization of a novel fibrinolytic enzyme, PSLTro01, from a medicinal animal Porcellio scaber Latreille

Tian

Лі

Li-wei

et al. 2015

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Purification and biochemical characterization of a novel fibrinolytic enzyme, PSLTro01, from a medicinal animal Porcellio scaber Latreille

Tian

Лі

Li-wei

et al. 2015

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

“…For example, a tripeptide (SQL) with antiplatelet activity was isolated from whole-body extracts of S. subspinipes mutilans [17,96]. It has been suggested that SQL might be a useful antithrombotic agent as it inhibits platelet aggregation in vitro and attenuates thrombus formation in vivo, without significant prolongation of bleeding time [17].…”

Section: Expect the Unexpected: Centipede-venom Compounds With Anti-tmentioning

confidence: 99%

Centipede venoms as a source of drug leads

Undheim

Jenner

King

2016

Expert Opinion on Drug Discovery

View full text Add to dashboard Cite

Introduction: Centipedes are one of the oldest and most successful lineages of venomous terrestrial predators. Despite their use for centuries in traditional medicine, centipede venoms remain poorly studied. However, recent work indicates that centipede venoms are highly complex chemical arsenals that are rich in disulfide-constrained peptides that have novel pharmacology and three-dimensional structure. Areas covered:This review summarizes what is currently know about centipede venom proteins, with a focus on disulfide-rich peptides that have novel or unexpected pharmacology that might be useful from a therapeutic perspective. We also highlight the remarkable diversity of constrained threedimensional peptide scaffolds present in these venoms that might be useful for bioengineering of drug leads. Expert opinion:The resurgence of interest in peptide drugs has stimulated interest in venoms as a source of highly stable, disulfide-constrained peptides with potential as therapeutics. Well-studied venomous taxa such as cone snails and snakes have yielded FDA-approved drugs, while ancient invertebrate predators such as spiders and sea anemones have yielded molecules that are currently in preclinical studies and clinical trials, respectively. However, until recently, the paucity of research on centipede venoms made it easy to discount them as a potential source of peptides with therapeutically useful properties. Seminal studies over the past five years have revealed that centipede venoms have exceedingly complex proteomes that are perhaps richer than any other venom in unique disulfide-rich peptide scaffolds. Like most arthropod predators, centipede venoms are rich in peptides that target neuronal ion channels and receptors, but it is also becoming increasingly apparent that many of these peptides have novel or unexpected pharmacological properties with potential applications in drug discovery and development.

show abstract

“…Anticoagulant peptides have mainly been reported from milk and its products (Shimizu et al, 2009). They have also been isolated from starfish (Koyama, Noguchi, Aniya, & Sakanashi, 1998), mussel (Jung & Kim, 2009), centipede (Kong, Huang, Shao, Li, & Wei, 2013), marine echiuroid worm (Jo et al, 2008), and silk worm (Kong et al, 2014), etc. However, there is paucity of information on anticoagulant activity of seaweed proteins.…”

Section: Introductionmentioning

confidence: 99%

“…Sometimes the trigger for anticoagulant activity may be from proteins or peptides or glycoprotein like compounds (Yasuda, Atsumi, Ieko, & Koike, 2004). The anticoagulant proteins or peptides have rarely been exploited from marine organisms (Jo et al, 2008;Jung, Je, Kim, & Kim, 2002;Jung & Kim, 2009;Kong et al, 2013;Rajapakse et al, 2005). The molecular interaction between the anticoagulant and clotting factors has been less documented.…”

Section: Introductionmentioning

confidence: 99%