Purification and Characterization of a Novel ~18 kDa Antioxidant Protein from Ginkgo biloba Seeds

Zhou, Hao; Chen, Xijuan; Wang, Chengzhang; Ye, Jianzhong; Chen, Hongxia

doi:10.3390/molecules171214778

Cited by 24 publications

(7 citation statements)

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“…6,11,12 However, bioactive peptides isolated from vegetable protein hydrolysates are becoming important, since they are a more economical and accessible alternative to proteins from animal sources. 13 Several studies have described the production of chelating peptides, which also exert antioxidant activity in the presence of transition metals, from proteins of plants such as Phaseolus vulgaris (bean), 14 Jatropha curcas (physic nut), 15 Helianthus annuus (sunflower), 16 Zea mays (corn), 17 Ginkgo biloba (maidenhair tree) 18 and Ipomoea batatas (sweet potato). 19 Also, in previous studies we showed that hydrolysis of Cicer arietinum L. (chickpea) proteins with the digestive enzymes pepsin and pancreatin produced copper-and iron-chelating peptides, which were purified by affinity chromatography through immobilized copper.…”

Section: Introductionmentioning

confidence: 99%

Chickpea chelating peptides inhibit copper‐mediated lipid peroxidation

2014

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BACKGROUND Transition metals produce radical oxygen species promoting lipid peroxidation processes that favor the development of cardiovascular and neurodegenerative diseases. In addition, the oxidation of lipids present in food may affect the quality of food products. Therefore antioxidants counteracting these metal pro‐oxidant effects may have high potential for the pharmacology and food industries. This study investigated the capability of peptide fractions purified from chickpea protein hydrolysate to inhibit copper‐mediated lipid peroxidation in three different lipid substrates: β‐carotene, unsaturated fatty acid mixture and low‐density lipoprotein. RESULTS Peptide fractions with the highest histidine content were the most antioxidant. This antioxidant effect is mainly due to the capability of histidine to bind copper and act as a hydrogen donor through its imidazole ring. CONCLUSION The results suggest that chickpea proteins are a potential source of antioxidant peptides that may be included as ingredients in functional foods with beneficial health effects. In addition, these antioxidant peptides may be useful to protect food products from lipid peroxidation processes and thus increase their quality and shelf life. © 2014 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Chickpea chelating peptides inhibit copper‐mediated lipid peroxidation

2014

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“…The protein content of Ginkgo biloba seed is around 10% (dry mass). Ginkgo biloba seed protein is considered to be a high-quality protein and exhibits strong antioxidant properties (Huang et al, 2010;Zhou et al, 2012). The protein polypeptide chain contains various functional areas that are considered bioactive with respect to antioxidant property.…”

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confidence: 99%

Purification and Identification of Novel Antioxidant Peptides from Enzymatic Hydrolysate of Ginkgo biloba Seed Proteins

Shi

Fan

et al. 2013

FSTR

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Ginkgo biloba seed proteins were hydrolyzed using alkali protease and pepsin to obtain antioxidant peptides. Ginkgo biloba antioxidant peptides (GKAPs) were separated by Sephadex G-25 and Sephadex G-10 gel filtration chromatography. The GKAP B3 exhibited highest antioxidant activity. B3 peptide was separated by reversed-phase high-performance liquid chromatography (RP-HPLC), and 2 peptides with good antioxidant activity, i.e., GKAPs C8 and C9, were obtained. The molecular weight and amino acid sequences of the peptides were identified by Liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). The results showed that the molecular mass of GKAP C8 was 452.21 Da and the amino acid sequence was YVGD (Tyr-Val-Gly-Asp), while the molecular mass of GKAP C9 was 988.49 Da and the amino acid sequence was LGNTDYAVH (Leu-Gly-Asn-Thr-Asp-Tyr-Ala-Val-His). GKAPs C8 and C9 exhibited good free radical-scavenging effect and inhibited the autoxidation of the linoleic acid system.Keywords: Ginkgo biloba seed protein, antioxidant peptide, radical scavenging activity, amino acid sequence *To whom correspondence should be addressed. E-mail: sxwucaie@163.com IntroductionIn China, Ginkgo biloba seeds are widely used in traditional medicines and foods. It is rich in vitamin C, riboflavin, carbohydrates, proteins, and many other nutrients (Son and Kim, 1998). The protein content of Ginkgo biloba seed is around 10% (dry mass). Ginkgo biloba seed protein is considered to be a high-quality protein and exhibits strong antioxidant properties (Huang et al., 2010;Zhou et al., 2012). The protein polypeptide chain contains various functional areas that are considered bioactive with respect to antioxidant property. When a protein polypeptide chain was hydrolyzed enzymatically, functional groups within the chain were exposed, and they can exhibit different biological activities (Meisel, 1997;Meisel and Bockelmann, 1999). In recent years, peptides generated from various species including animal and plant sources have been found to possess good antioxidant activity. For example, peptides ACGT and ATAGT from Tibpia frame protein (Fan et al., 2012), peptide LEELEEELEGCE from Bullfrog skin (Qian et al., 2008b), LPHSGY from Alaska Pollock protein hydrolysate (Je et al., 2005), NADFGLNGLEGLA from Giant squid, and LKQELEDLLEKQE from Oyster protein hydrolysate have been reported to possess antioxidant activity (Qian et al., 2008a;Rajapakse et al., 2005). In these studies, the antioxidant activity of peptides was evaluated by scavenging free radicals by in vitro assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, hydroxyl (•OH) radical, superoxide anion radical (•O 2 ), and determination of antioxidant capacity in linoleic acid system. However, there are few reports on antioxidant peptides from Ginkgo biloba seed peptides. In this research, Ginkgo biloba seed protein was hydrolyzed with alkaline protease 2709 and pepsin to obtain antioxidant peptides. The antioxidant activity of antioxidant peptides were evaluated b...

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“…Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) has become an important tool of choice for large molecular analyses, especially for proteins. The proteome profiling technique by MALDI-TOF-MS provided a broad-base and effective approach for the identification of proteins with biological activity [ 24 , 25 ]. In the present investigation the gel band of F3 was excised from Coomassie Blue-stained gel and further processed for MALDI-TOF-MS analysis.…”

Section: Resultsmentioning

confidence: 99%

Induction of Apoptotic Effects of Antiproliferative Protein from the Seeds ofBorreria hispidaon Lung Cancer (A549) and Cervical Cancer (HeLa) Cell Lines

Rupachandra

Sarada

2014

BioMed Research International

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A 35 KDa protein referred to as F3 was purified from the seeds of Borreria hispida by precipitation with 80% ammonium sulphate and gel filtration on Sephadex G-100 column. RP-HPLC analysis of protein fraction (F3) on an analytical C-18 column produced a single peak, detected at 220 nm. F3 showed an apparent molecular weight of 35 KDa by SDS PAGE and MALDI-TOF-MS analyses. Peptide mass fingerprinting analysis of F3 showed the closest homology with the sequence of 1-aminocyclopropane-1-carboxylate deaminase of Pyrococcus horikoshii. The protein (F3) exhibited significant cytotoxic activity against lung (A549) and cervical (HeLa) cancer cells in a dose-dependent manner at concentrations ranging from 10 µg to 1000 µg/mL, as revealed by the MTT assay. Cell cycle analysis revealed the increased growth of sub-G0 population in both cell lines exposed to a concentration of 1000 µg/mL of protein fraction F3 as examined from flow cytometry. This is the first report of a protein from the seeds of Borreria hispida with antiproliferative and apoptotic activity in lung (A549) and cervical (HeLa) cancer cells.

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Purification and Characterization of a Novel ~18 kDa Antioxidant Protein from Ginkgo biloba Seeds

Cited by 24 publications

References 40 publications

Chickpea chelating peptides inhibit copper‐mediated lipid peroxidation

Chickpea chelating peptides inhibit copper‐mediated lipid peroxidation

Purification and Identification of Novel Antioxidant Peptides from Enzymatic Hydrolysate of Ginkgo biloba Seed Proteins

Induction of Apoptotic Effects of Antiproliferative Protein from the Seeds ofBorreria hispidaon Lung Cancer (A549) and Cervical Cancer (HeLa) Cell Lines

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