Purification and characterization of a carboxylesterase associated with organophosphate resistance in the greenbug,Schizaphis graminum(Homoptera: Aphididae)

Siegfried, Blair D.; Ono, Midori; Swanson, Jeffrey J.

doi:10.1002/(sici)1520-6327(1997)36:3<229::aid-arch7>3.0.co;2-o

Cited by 13 publications

(1 citation statement)

References 18 publications

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“…It follows that experiments with crude extracts may produce misleading results. However, AChEs have been purified from only a few insect species, including Musca domestica (Fournier et al, 1987), Lygus hesperus Knight (Zhu et al, 1992), Leptinotarsa decemlineata (Zhu and Clark, 1994), Helicoverpa armigera (Gao et al, 1998a), Diabrotica virgiferavirgifera (Gao et al, 1998b) Galleria mellonella L. (Sharon et al, 1999), Blattella germanica (Prabhakaran and Kamble, 1996) and the greenbug, Schizaphis graminum (Siegfried et al, 1997). Here, we report the purification of AChE from the cotton aphid, Aphis gossypii Glover, by using procainamide affinity column chromatography, and its characterization by additional pharmacological experiments.…”

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confidence: 99%

Purification and characterization of acetylcholinesterase from cotton aphid (Aphis gossypii Glover)

Han

2002

Arch Insect Biochem Physiol

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A simple and effective method was set up to purify acetylcholinesterase (AChE, EC3.1.1.7) from the cotton aphid, Aphis gossypii Glover. The procedure involved filtration on a sephadex G-25 column, separation with sephadex G-200 and procainamide affinity column. AChE from both susceptible and resistant strains were purified to a single band as resolved on denaturing polyacrylamide gel electrophoresis (SDS-PAGE). The specific activity increased by 35,100- and 33,680-fold with a yield of 30.3 and 29.8%, respectively. The molecular mass of the purified AChE was about 63,500 Dalton as determined by SDS-PAGE. However, three bands resolved on PAGE gel electrophoresis, leading to the inference that native AChE exists in three forms. The optimum conditions for measuring the activity of purified AChE with kinetic method were 0.02M phosphate buffer, pH7.2, 0.02 mM 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), and 25 degrees C. Investigation also revealed that crude extract and purified AChE had different kinetic characteristics and inhibitory properties. They responded differently to varied DTNB, ATChI, and phosphate buffer ion concentrations, as well as pH, temperature, and inhibitors. The purified AChE was more sensitive to eserine, methamidophos, and pirimicarb. Especially for resistant aphids, the sensitivity of purified AChE to methamidophos and pirimicarb was enhanced 6.43 and 11.73 times, respectively. We infer that one or more factors in the crude extract from the resistance strain have more influence on AChE sensitivity. Further study is needed to investigate the basis of these observations.

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Section: Introductionmentioning

confidence: 99%

Purification and characterization of acetylcholinesterase from cotton aphid (Aphis gossypii Glover)

Han

2002

Arch Insect Biochem Physiol

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Elevated Esterases Exhibiting Arylesterase-like Characteristics in an Organophosphate-Resistant Clone of the Greenbug, Schizaphis graminum (Homoptera: Aphididae)

Zhu

2000

Pesticide Biochemistry and Physiology

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Two Qualitatively Different B-Esterases from Two Organophosphate-Resistant Strains of Oryzaephilus surinamensis (Coleoptera: Silvanidae) and Their Roles in Fenitrothion and Chlorpyrifos-Methyl Resistance

Rossiter

Conyers²,

MacNicoll³

et al. 2001

Pesticide Biochemistry and Physiology

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Purification and characterization of a carboxylesterase associated with organophosphate resistance in the greenbug,Schizaphis graminum(Homoptera: Aphididae)

Cited by 13 publications

References 18 publications

Purification and characterization of acetylcholinesterase from cotton aphid (Aphis gossypii Glover)

Purification and characterization of acetylcholinesterase from cotton aphid (Aphis gossypii Glover)

Elevated Esterases Exhibiting Arylesterase-like Characteristics in an Organophosphate-Resistant Clone of the Greenbug, Schizaphis graminum (Homoptera: Aphididae)

Two Qualitatively Different B-Esterases from Two Organophosphate-Resistant Strains of Oryzaephilus surinamensis (Coleoptera: Silvanidae) and Their Roles in Fenitrothion and Chlorpyrifos-Methyl Resistance

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