Purification and characterization of a solublepolyurethane degrading enzyme from Comamonasacidovorans

Allen, April B.; Hilliard, Newton P.; Howard, Gary T.

doi:10.1016/s0964-8305(98)00066-3

Cited by 53 publications

(26 citation statements)

References 15 publications

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“…However, esterase activity was detected in Tween 80 media, and by using the p-NPA assay, the esterase activity secreted to the culture medium was measured. A lipase activity has been identified in Bacillus subtilis (35), and extracellular esterase activities have been reported for Pseudomonas chlororaphis and Comamonas acidovorans growing in media with Impranil (2,20,36). In C. acidovorans TB-35, an extracellular esterase and a membrane-associated esterase have been identified (30).…”

Section: Discussionmentioning

confidence: 98%

“…This genus is phylogenetically related to Acidovorax defluvii and Comamonas denitrificans, which have previously been isolated from activated sludge (15,40). Moreover, another bacterium of the Comamonadaceae family, C. acidovorans, has been reported as being able to use various types of PU to sustain its growth (2,30).…”

Section: Discussionmentioning

confidence: 98%

“…The samples were flexible PU foam pieces collected from the ground or deteriorated upholstery. They were carried in plastic bags to the laboratory, and portions were introduced to 125-ml Erlenmeyer flasks containing 50 ml minimal medium (MM) at pH 7. is an aliphatic PU water-based coating similar to Impranil DLN (Bayer Corp.), which has been employed in other experimental work to characterize polyurethanolytic bacteria (2,19,20,35,36). The inoculated media were incubated for 5 days at 37°C and at 200 rpm.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of the Polyurethanolytic Activity of Two Alicycliphilus sp. Strains Able To Degrade Polyurethane and N -Methylpyrrolidone

Oceguera-Cervantes

Carrillo-García

López

et al. 2007

Appl Environ Microbiol

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Two bacterial strains (BQ1 and BQ8) were isolated from decomposed soft foam. These were selected for their capacity to grow in a minimal medium (MM) supplemented with a commercial surface-coating polyurethane (PU) (Hydroform) as the carbon source (MM-PUh). Both bacterial strains were identified as Alicycliphilus sp. by comparative 16S rRNA gene sequence analysis. Growth in MM-PUh showed hyperbolic behavior, with BQ1 producing higher maximum growth (17.8 ؎ 0.6 mg ⅐ ml ؊1 ) than BQ8 (14.0 ؎ 0.6 mg ⅐ ml ؊1 ) after 100 h of culture. Nuclear magnetic resonance, Fourier transform infrared (IR) spectroscopy, and gas chromatographymass spectrometry analyses of Hydroform showed that it was a polyester PU type which also contained N-methylpyrrolidone (NMP) as an additive. Alicycliphilus sp. utilizes NMP during the first stage of growth and was able to use it as the sole carbon and nitrogen source, with calculated K s values of about 8 mg ⅐ ml ؊1 . Enzymatic activities related to PU degradation (esterase, protease, and urease activities) were tested by using differential media and activity assays in cell-free supernatants of bacterial cultures in MM-PUh. Induction of esterase activity in inoculated MM-PUh, but not that of protease or urease activities, was observed at 12 h of culture. Esterase activity reached its maximum at 18 h and was maintained at 50% of its maximal activity until the end of the analysis (120 h). The capacity of Alicycliphilus sp. to degrade PU was demonstrated by changes in the PU IR spectrum and by the numerous holes produced in solid PU observed by scanning electron microscopy after bacterial culture. Changes in the PU IR spectra indicate that an esterase activity is involved in PU degradation.Polyurethane (PU) was developed by Otto Bayer as a substitute for rubber at the beginning of World War II (1938). Due to its range of properties, the polymer is widely used, for example, in liquid coatings and paints, adhesives, sealants, flexible and rigid foams, and elastomers (7). Since PU is such a versatile polymer, PU production has increased, but this has brought with it the problem of safe disposal. Each year, more than 5 million tons of shredder residue containing different plastics and PU foams is generated in the United States and Canada (6). Several mechanical processes, such as regrinding, flexible foam bonding, adhesive pressing, and compression molding, as well as chemical techniques, such as glycolysis, hydrolysis, pyrolysis, and hydrogenation, are used for the recovery of the starting materials or in the production of other PU types (8). Recently, the development of new strategies based on the utilization of biopolymers such as poly[(R)-hydroxyalkanoic acids] (21), the enzymatic polymerization of polyesters and degradation of PU (28), and the discovery of microorganisms (fungi and bacteria) able to utilize PU as a source of carbon and nitrogen (12,31,32) is leading the move to a greener chemical industry.A number of bacterial strains, such as Corynebacterium sp., Pseudomonas fluorescens, P. ch...

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of the Polyurethanolytic Activity of Two Alicycliphilus sp. Strains Able To Degrade Polyurethane and N -Methylpyrrolidone

Oceguera-Cervantes

Carrillo-García

López

et al. 2007

Appl Environ Microbiol

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“…This fibre has a very large extension at rupture, colour permanence, and resistance to radiation and ageing. Microbial degradation of polyurethanes occurs by way of chemical hydrolysis, as a result of the extracellular action of esterase enzymes (Akutsu et al, 1998;Allen et al, 1990;Ruiz et al, 1999).…”

Section: Microbial Degradation Of Fibersmentioning

confidence: 99%

Microbial Degradation of Woven Fabrics and Protection Against Biodegradation

Gutarowska¹,

Michalski²

2012

Woven Fabrics

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“…The world consumption of plastic materials in agriculture amounts yearly to 6.5 million tons, more than 10% of the total consumption refers to plastic films for soil mulching (Liu et al, 2010). Biodegradable plastics has gained importance, which gets degraded in litter in few months (6-8 months) to years (2-5 years) depending on type, thickness and gauze (Allen et al, 1999). Only microorganisms have capacity to utilize Polyurethane (PU) as a sole source of carbon and nitrogen and degrading it by polyurthenase and other enzymes (Howard, 2003).…”

Section: Introductionmentioning

confidence: 99%

Diversity of Plastic Degrading Microorganisms and Their Appraisal on Biodegradable Plastic

K¹

2013

Appl Ecol Env Res

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Abstract. Efforts on isolation of native plastic adoring beneficial microorganism from landfills of three different geographical niches were carried out following testing of their ability to degrade biodegradable polyethylene in laboratory appraisal of identified native microorganisms; Aspergillus terreus Gr., Aspergillus wentii Gr., Emericella nidulans (Eidam), Pseudomonas sp. and Streptomyces sp., on different biodegradable polyethylene showed biodegradability in terms of weight loss of plastic in liquid with biofilm formation on plastic strips and it was also noticed that bacteria remained more active compared to fungi in biodegradation process. Amylase production by proven microorganisms showed that A.terreus produced maximum amylase followed by E.nidulans, A.wentii, Pseudomonas sp. and Streptomyces sp. Bio-efficacy of microorganisms against different BD PE in pots indicated that colonization of plastic was not found up to 3 month interval by all of them but E.nidulans found to colonizing IPCL plastic and A.wentii colonizing Israel plastic at 6 month interval.

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Purification and characterization of a solublepolyurethane degrading enzyme from Comamonasacidovorans

Cited by 53 publications

References 15 publications

Characterization of the Polyurethanolytic Activity of Two Alicycliphilus sp. Strains Able To Degrade Polyurethane and N -Methylpyrrolidone

Characterization of the Polyurethanolytic Activity of Two Alicycliphilus sp. Strains Able To Degrade Polyurethane and N -Methylpyrrolidone

Microbial Degradation of Woven Fabrics and Protection Against Biodegradation

Diversity of Plastic Degrading Microorganisms and Their Appraisal on Biodegradable Plastic

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