Purification and Characterization of a Mitogenic Lectin and a Lectin-Binding Protein fromVicia sativa

<i>In vitro</i> antibacterial activity and phytochemical analysis of hexane extract of <i>Vicia sativa</i>

“…Previous phytochemical studies showed that V. sativa contains various constituents e.g, apigenin (Boulos, 1995), kaempferol (Tschiersch and Hanelt, 1966), Luteolin (Seabra et al, 2001), quercetin (Roy et al, 1996), these compounds are identified by NMR-spectroscopy (Agrawal, 1989). Lectin also has been isolated from the plant (Gebauer et al, 1979).…”

The Amino‐Acid Sequence of the α Subunit of the Mitogenic Lectin from Vicia sativa

“…The a subunit was isolated in micromole amounts by Biogel P-10 chromatography and checked for purity and molecular weight according to [3]. Sodium propionate and urea were removed by gel filtration on Sephadex G-25 superfine (2.1 cm x 50 cm) in 0.1 M NH,.…”

“…The incubation was continued for 30min at 50°C. For the determination of the N-terminal sequence, the x subunit was attached to isothiocyanate glass carrier as described earlier [3]. Automatic Edman degradation [8] was performed in a solid-phase sequencer (Sequemat Inc., Watertown, MA, USA, model 12 S).…”

“…The small amount of T 3 which appeared in the void volume of the gel filtration was not recovered from Aminex A5 pH 3 with formic acid. Both peptides were then sequenced together and TI a could in turn be subtracted because its sequence was known [3]. T3a aiid TI b, too, were sequenced as a mixture; however, since purified TI b was also obtained (see Fig.…”

“…This often leads to agglutination or mitogenesis in animal cells [l, 21. In a previous paper [3], the purification of a lectin from Vicia sativa subspecies angustifolia was described which agglutinates red blood cells and stimulates mitosis in lymphocytes nearly as effectively as concanavalin A, the lectin from Canavalia ensformis. The V. sativa lectin was resolved into its subunits; it appears to be an a2P2 complex ( M , 40000).…”