2003
DOI: 10.1128/aem.69.10.5546-5553.2003
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Purification and Characterization of a Novel Bacteriocin Produced by Enterococcus faecalis Strain RJ-11

Abstract: Lactic acid bacteria exhibiting activity against the gram-positive bacterium Bacillus subtilis were isolated from rice bran. One of the isolates, identified as Enterococcus faecalis RJ-11, exhibited a wide spectrum of growth inhibition with various gram-positive bacteria. A bacteriocin purified from culture fluid, designated enterocin RJ-11, was heat stable and was not sensitive to acid and alkaline conditions, but it was sensitive to several proteolytic enzymes. Sodium dodecyl sulfate-polyacrylamide gel elect… Show more

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Cited by 101 publications
(73 citation statements)
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“…A total of 4 ml of overnight culture was then inoculated into 200 ml of fresh Todd-Hewitt medium and incubated for 24 h at 37°C in a shaker incubator at 150 rpm. An equal volume of chemically defined media (7) was then added and incubated for another 24 h. Bacterial culture was then passed through a 0.45-m membrane filter, followed by ammonium sulfate fractionation (80% saturation) as described by Yamamoto et al (31).…”
Section: Methodsmentioning
confidence: 99%
“…A total of 4 ml of overnight culture was then inoculated into 200 ml of fresh Todd-Hewitt medium and incubated for 24 h at 37°C in a shaker incubator at 150 rpm. An equal volume of chemically defined media (7) was then added and incubated for another 24 h. Bacterial culture was then passed through a 0.45-m membrane filter, followed by ammonium sulfate fractionation (80% saturation) as described by Yamamoto et al (31).…”
Section: Methodsmentioning
confidence: 99%
“…Arbitrary units (AU) of bacteriocin activities were calculated according to spot-on-lawn method (14). The resulting sample was serially diluted two-fold with filter-sterilized phosphate buffer (20 mM, pH 6.5).…”
Section: Microorganismsmentioning
confidence: 99%
“…Evaluation of the bacteriocin content was performed by isolating an aliquot of the culture medium followed by centrifugation (20 min, 4 °C, 9000 g) and then fi ltering through a Durapore® PVDF fi lter with pore size 0.22 μm (Millipore). Activity of bacteriocins was expressed in arbitrary units per millilitre, calculated according to formula [17] At the next step the desalting of the culture medium was performed using a reversed-phase lowpressure chromatography on a Brownlee Aquapore RP-300 column (PerkinElmer, USA) equilibrated with solvent A (10% acetonitrile (Merck, Germany) in ultra-purifi ed water (18 ohms, Milli-Q, Millipore) with 0.1% trifl uoroacetic acid (TFA). Elution was performed using the solvent B (80% acetonitrile in ultra-purifi ed water with 0.1% TFA) followed by lyophilization to withdraw a residual quantity of TFA and concentration.…”
Section: Materials and Methods Bacterial Strains Growth Conditions mentioning
confidence: 99%