Purification and characterization of a novel laccase from the edible mushroom Clitocybe maxima

Zhang, Guoqing; Wang, Yifan; Zhang, Xiaoqing; Ng, Tzi Bun; Wang, Hexiang

doi:10.1016/j.procbio.2009.12.010

Cited by 102 publications

(80 citation statements)

References 51 publications

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“…Relative activities at 15,20,30,40,50, and 60°C were 67.0 ± 1.0, 72.8 ± 0.3, 84.2 ± 1.9, 63.0 ± 2.7, 50.5 ± 0.8, and 47.6 ± 0.7%, respectively, compared to that at ambient temperature. The crude laccase was found to be relatively stable at room temperature (25 ± 2°C), but its activity declined quickly as the storage temperatures rose above 30°C (Fig.…”

Section: Fungal Collection and Screening For Laccase Producermentioning

confidence: 88%

“…In contrast, CuSO 4 or MnSO 4 (10 mM) showed no inhibition to the enzyme activity. In comparison to the laccases from other white-rot fungi [28][29][30] including an isolate of Pycnoporus 31 , it was found that P. coccineus Thongkred 013 BCU laccase was more sensitive to SDS, EDTA, and almost all ions tested, except for Mn 2+ .…”

Section: Fungal Collection and Screening For Laccase Producermentioning

confidence: 90%

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Thongkred¹,

Lotrakul²,

Prasongsuk³

et al. 2011

ScienceAsia

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Basidiocarps of Pycnoporus coccineus and P. sanguineus were collected from Kanchanaburi, Phetchaburi, and Mae Hong Son provinces in Thailand. Liquid culture of P. coccineus isolates provided higher laccase and manganese peroxidase activities than those of P. sanguineus. Lignin peroxidase activity was not detectable in any of the three Pycnoporus isolates. P. coccineus Thongkred 013 BCU, the best laccase producer among these isolates, was selected to optimize laccase production. The highest laccase yield (5.97 ± 0.40 U/ml) was obtained when grown at 28°C for 8 days in basal medium containing 2% (w/v) glucose and 0.25% (w/v) peptone at an initial pH of 5.0. The optimum conditions for stability and activity of the crude laccase enzyme were at pH 3.5 and room temperature (25 ± 2°C). FeSO4 (10 mM) and SDS (1% w/v) were both inhibitory to the laccase activity. When five different types of polycyclic aromatic hydrocarbons (PAHs) were individually added to liquid culture of P. coccineus at 100 ppm, the oxidation of anthracene, pyrene, and fluoranthene detected within 24 h were 59.7, 50.7, and 49.8%, respectively, whereas only 25.3% of benzo[a]pyrene and 32.4% of phenanthrene were oxidized. When P. coccineus crude laccase (1 U/ml) was used, these PAHs were more readily oxidized, with 76.4 and 74.3% of pyrene and anthracene, respectively, being oxidized within 2 h and 84.2% of fluoranthene oxidized within 24 h. Benzo[a]pyrene and phenanthrene oxidation by the enzyme were enhanced when 1 mM 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) was added as a mediator to the reaction mixture, resulting in 66.3 and 50.5% of the initial 100 ppm being oxidized after 24 h.

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Section: Fungal Collection and Screening For Laccase Producermentioning

confidence: 88%

Section: Fungal Collection and Screening For Laccase Producermentioning

confidence: 90%

Untitled

Thongkred¹,

Lotrakul²,

Prasongsuk³

et al. 2011

ScienceAsia

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“…The homogenates were centrifuged and precipitates were removed. For the purification of the laccase enzyme the following procedure was implemented [22]. Laccase was purified from the supernatant in two steps.…”

Section: Methodsmentioning

confidence: 99%

“…After mixing in an ice-bath for 1 h with magnetic stirring, it was centrifuged (10,000 × g, 30 min, and 4ºC). The supernatant was discarded and the precipitate was dissolved in 0.01 M acetate buffer (pH 5.0) and dialyzed against the same buffer [22].…”

Section: Methodsmentioning

confidence: 99%

Experimental Study to Remediate Acid Fuchsin Dye Using Laccase-Modified Zeolite from Aqueous Solutions

Kalkan

Nadaroğlu

Çelebi

et al. 2015

Pol. J. Environ. Stud.

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“…A B The purified laccase from FBV40 exhibited the highest activity toward ABTS, similar to laccases from other fungi, such as C. maxima [7], Fusarium solani and Agrocybe cylindracea [8]. Using ABTS as the substrate, the enzyme from Trametes Versicolor IBL-04 showed V max of 780 U/mL with a corresponding K m value of 73 µM [9].…”

Section: Discussionmentioning

confidence: 91%

Purification and Characterization of Laccase Involved in the Decolourization of Synthetic Dyes and 2,3,7,8-TCDD Congener Degradation by the White Rot Fungus Isolated From Bavi Forest of Vietnam

Chu¹

2018

JST

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The fungal strain FBV40 was isolated from soil containing decayed wood in Ba Vi National Forest and capable of producing an extracellular laccase in the TSH1 medium. Two isozyme such as Lac1 and Lac2 were purified were estimated to be 55 and 60 kDa by SDS-PAGE. The optimum pH and temperature for the enzymatic activity of Lac1 were 3.0 and 60°C with ABTS using as the substrate. Kinetic constants K m and V max of Lac 1 were 0.3 µM and 200,000 µM/mins with ABTS as substrate. Cl -, SDS, and EDTA at any concentration (2 mM; 5 mM and 10 mM) strongly inhibited the activity of laccase. The enzyme was stable in the presence of several metal ions including Ni 2+ (1 mM), Cu 2+ (1 mM and 3 mM), Ca 2+ (3 mM and 4 mM); in the presence of Cu 2+ (2 mM) and Ca 2+ (0.5 mM, 1.0 mM and 2.0 mM), laccase even showed the increase in the activity. The presence of metal ions Mn 2+ , Mg 2+ , Fe 2+ completely inhibited the enzymatic activity at any examined concentration. The crude enzyme, as well as Lac 1, was able to decolourization MN.FBN dye from the textile industry from Ministry of Defence. This strain was able to degrade 2,3,7,8-TCDD isotop with initial concentration 2,000 ng-TEQ/L at rates over 46.8 % after ten days cultivation in the TSH1 medium. In the presence of three strains FBV40, FBVLa1 and FBD154 with the ratio 1:1:1, the degradation of this congener was achieved more than 95 % at the same time cultivation.

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Purification and characterization of a novel laccase from the edible mushroom Clitocybe maxima

Cited by 102 publications

References 51 publications

Untitled

Untitled

Experimental Study to Remediate Acid Fuchsin Dye Using Laccase-Modified Zeolite from Aqueous Solutions

Purification and Characterization of Laccase Involved in the Decolourization of Synthetic Dyes and 2,3,7,8-TCDD Congener Degradation by the White Rot Fungus Isolated From Bavi Forest of Vietnam

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