Purification and Characterization of a new Lectin from the Red Marine Alga Hypnea Musciformis

Nagano, Celso Shiniti; Moreno, Frederico Bruno Mendes Batista; Bloch, Carlos; Prates, Maura V.; Calvete, Juan J.; Saker-Sampaio, Silvana; Farias, Wladimir Ronald Lobo; Tavares, Talita de Sousa; Nascimento, Kyria S.; Grangeiro, Thalles Barbosa; Cavada, B.S.; Sampaio, Alexandre Holanda

doi:10.2174/0929866023408931

Cited by 36 publications

(19 citation statements)

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“…Moreover, they do not appear to require metal ions, showing a high content of acidic residues and, even at high concentrations, tend to stay in the monomeric form (2)(3)(4). It has been shown (5) that aqueous extracts of the red marine algae Hypnea musciformis and H. cervicornis present hemagglutinating activity, and a recent study (6) reported the purification and characterization of a new lectin from H. musciformis that agglutinates erythrocytes from various sources.…”

Section: Introductionmentioning

confidence: 99%

Antinociceptive properties in mice of a lectin isolated from the marine alga Amansia multifida Lamouroux

Neves

Freitas

Sousa

et al. 2007

Braz J Med Biol Res

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The antinociceptive effects of a lectin (LEC) isolated from the marine alga Amansia multifida were determined in Swiss mice. The LEC (1, 5, and 10 mg/kg) inhibited acetic acid-induced abdominal writhings in a dose-dependent manner after intraperitoneal or oral administration. A partial but significant inhibition of writhings was observed after the combination of LEC (10 mg/kg) with avidin (1 mg/kg), a potent inhibitor of the hemmaglutinant activity of the lectin. However, total writhing inhibition was demonstrable in the group of mice treated with LEC plus mannose (1 mg/kg), as compared to LEC alone or to control groups. Furthermore, avidin and mainly mannose also play a role in antinociception, somehow facilitating the interaction of LEC with its active cell sites. In the formalin test, although both phases of the response were significantly inhibited, the effect of LEC was predominant during phase 2, causing inhibition of licking time that ranged from 48 to 88% after oral (5 and 10 mg/kg) and intraperitoneal (1 to 5 mg/kg) administration. As is the case with morphine, the effect of LEC (2 mg/kg) was reversed by naloxone (2 mg/kg), indicating the involvement of the opioid system. LEC was also effective in the hotplate test, producing inhibitory responses to the thermal stimulus, and its effects were blocked by naloxone. In the pentobarbital-induced sleeping time, although LEC did not alter the onset of sleep significantly, it increased the time of sleep within the same dose range compared to control. These results show that LEC presents antinociceptive effects of both central and peripheral origin, possibly involving the participation of the opioid system.

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Section: Introductionmentioning

confidence: 99%

Antinociceptive properties in mice of a lectin isolated from the marine alga Amansia multifida Lamouroux

Neves

Freitas

Sousa

et al. 2007

Braz J Med Biol Res

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“…Lectins from marine algae Cystoclonium purpureum [74], Gracilaria verrucosa [75], Palmaria palmata [76], Solieria robusta [62], Gracilaria tikvahiae [60], Bryothamnion seaforthii and B.triquetrum [66], Solieria filiformis [77], Enantiocladia duperreyi [78], Amansia multifida [73], Hypnea musciformis [79], Gracilaria ornata [80], Hypnea cervicornis [81] and Georgiela confluens [82] were isolated by exchange chromatography, usually on DEAE cellulose. In contrast, due usually algal lectins have binding specificity of complex sugars, affinity chromatography has been used a few times, such as the lectins of green algae of the genus Codium [83,84,85], lectins from Ulva lactuca [86], Caulerpa cupressoides [87], Enteromorpha prolifera [88], Ulva pertusa [89], Bryopsis plumosa [90,91,92], Bryopsis hypnoides [93] and lectins from red marine algae of the genus Ptilota [68,69,70].…”

Section: Marine Algae Lectinsmentioning

confidence: 99%

Biological Applications of Plants and Algae Lectins: An Overview

Teixeira¹,

Arruda²,

Nascimento³

et al. 2012

Carbohydrates - Comprehensive Studies on Glycobiology and Glycotechnology

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“…H. musciformis has great economic value for the production of κ-carrageenan, an industrially important sulfated polysaccharide (Oliveira, 1998;Reis et al, 2006), and has shown antiviral activity against HSV (herpes simplex virus) and HIV (human immunodeficiency virus) (Neushul, 1990). H. musciformis also contains lectins, which have anti-inflammatory and hemagglutinating activity, besides being useful in cancer diagnosis (Nagano et al, 2002;Nagano et al, 2005). Extracts of H. musciformis also proved to have anthelmintic, anti-inflammatory, myo-relaxing and antifungal action (Salimabi, 1980;Davies et al, 1984;Melo et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the photosynthetic conditions and pigment profiles of the colour strains of Hypnea musciformis from field-collected and in vitro cultured samples

Fernandes¹,

Caetano²,

Reinert³

et al. 2012

Rev. bras. farmacogn.

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Abstract:Hypnea musciformis (Wulfen) JV Lamour. is a species of great economic interest as it produces κ-carrageenan and has shown biological activities against HIV and HSV viruses. This species displays different colour strains in its natural habitat, which may have implications for the biotechnological potential of the species. The aim of this study was to characterize the photosynthetic apparatus and pigment profile of three colour strains of H. musciformis (green, brown and red) in their natural habitat and in culture. Chlorophyll a fluorescence of photosystem II was measured with a pulse-amplitude modulated fluorometer and pigments were quantified by spectrofluorimetry (chlorophyll a) and spectrophotometry (phycobiliproteins). In the natural habitat, we detected significant differences between the colour strains for the following photochemical parameters: the green strain had a higher effective quantum yield (ΦPSII) than the red strain and a higher maximum relative electron transport rate (rETRmax) than the brown and red strains. Saturation irradiances were 1000 μE.m -2 .s -1 (green) and 500 μE.m -2 .s -1 (brown and red). Concerning in vitro culture, the green strain presented the lowest ΦPSII, rETRmax, and α rETR, while the brown strain presented the highest values for these same parameters. The chlorophyll a content of the cultured green strain was the lowest. The phycoerythrin contents of the three colour strains were unchanged by either natural of in vitro conditions: lower in green, intermediate in brown and higher in the red strain, ensuring the chromatic identity of the strains. Our results suggest that the green strain has a better performance when exposed to high irradiance, but a lower efficiency under low irradiance compared to the brown and red strains.

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Purification and Characterization of a new Lectin from the Red Marine Alga Hypnea Musciformis

Cited by 36 publications

References 0 publications

Antinociceptive properties in mice of a lectin isolated from the marine alga Amansia multifida Lamouroux

Antinociceptive properties in mice of a lectin isolated from the marine alga Amansia multifida Lamouroux

Biological Applications of Plants and Algae Lectins: An Overview

Characterization of the photosynthetic conditions and pigment profiles of the colour strains of Hypnea musciformis from field-collected and in vitro cultured samples

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