Purification and characterization of a serine protease secreted from Brevibacillus sp. KH3 for reducing excess sludge and biofilm formation

Maeda, Toshinari; Yoshimura, Takayuki; Ogawa, Hiroaki

doi:10.1016/j.jbiosc.2009.08.236

Cited by 1 publication

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“…Previous studies have shown that most keratinases are serine proteases (Gradišar et al, 2005). The main industrial producers of serine proteases are strains belonging to the genera Bacillus (Jaouadi et al, 2010;Maeda et al, 2011;da Silva, 2018), and Streptomyces (Demir et al, 2015). Among all the reported keratinases, two have been reported to be produced by members of the genus Lysobacter.…”

Section: Discussionmentioning

confidence: 99%

A Newly Isolated Strain Lysobacter brunescens YQ20 and Its Performance on Wool Waste Biodegradation

Zhang

Zheng

et al. 2022

Front. Microbiol.

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Wool keratin is difficult to degrade as comparing to feathers because of its tough secondary structure. In order to develop an approach for high-value utilization of wool fiber waste by keratinolytic microorganisms, which is produced from shearing, weaving, and industrial processing of wool, screening of wool-degrading bacterium with high degradation efficiency were performed in this study. To this end, Lysobacter brunescens YQ20 was identified and characterized. The optimized conditions for wool degradation were pH 9.0 and 37°C with 20% liquid volume of Erlenmeyer flask. After fermentation, 15 essential amino acids were detected when wool fiber waste was fermented. The total amino acids produced from 1% wool per hour were 13.7 mg/L. The concentration was 8.6-fold higher than that produced by the strain Stenotrophomonas maltophilia BBE11-1, which had previously been reported to have the highest wool-degrading capacity. Our study reports the first Lysobacter strain that exhibits efficient wool degradation and yields higher concentrations of amino acids than previously reported strains. Whole-genome sequencing indicated that there were 18 keratinase-like genes in the genome of YQ20, which exhibited a long evolutionary distance from those of Bacillus. Therefore, L. brunescens YQ20 may have applications in the environmentally friendly management of wool waste as fertilizer in agriculture.

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