Purification and Characterization of a Metalloprotease fromChryseobacterium indologenesIx9a and Determination of the Amino Acid Specificity with Electrospray Mass Spectrometry

Venter, Henrietta; Osthoff, Gernot; Litthauer, Derek

doi:10.1006/prep.1998.1020

Cited by 32 publications

(17 citation statements)

References 48 publications

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“…Their optimal temperatures were also within the range commonly described for bacterial keratinases, 30-50, 40 and 40-50°C, respectively. An acidophilic protease has already been described for C. indologenes strain Ix9a, presenting optimal activity at pH 6.5 and 50°C (Venter et al 1999).…”

Section: Resultsmentioning

confidence: 99%

Production and properties of keratinolytic proteases from three novel Gram-negative feather-degrading bacteria isolated from Brazilian soils

et al. 2011

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The keratinolytic potential and protease properties of three novel Gram-negative feather-degrading bacteria isolated from Brazilian soils was described. Aeromonas hydrophila K12, Chryseobacterium indologenes A22 and Serratia marcescens P3 were able to degrade feather meal, producing high amounts of soluble proteins and forming thiol groups. The proteases of strains K12, A22 and P3 had optimal pH of 8.0, 7.5 and 6.0, respectively; this last is an uncommon feature for bacterial keratinases. The optimal temperature was in the range 45-55°C. All three proteases were active towards azokeratin and were inhibited by EDTA, suggesting that they are keratinolytic metalloproteases. The proteolytic activity of K12 was stimulated by organic solvents and the detergent SDS, suggesting its potential application for detergent formulations and peptide synthesis. Strains A22, K12 and P3 have great potential for use in biotechnological processes involving hydrolysis of keratinous byproducts.

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Section: Resultsmentioning

confidence: 99%

Production and properties of keratinolytic proteases from three novel Gram-negative feather-degrading bacteria isolated from Brazilian soils

et al. 2011

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“…A metalloprotease of C. indologenes Ix9a (Venter et al 1999) and an endopeptidase of Chryseobacterium sp. (Lijnen et al 2000) were produced during cultivation in nutrient broth at 25ºC and 28ºC, respectively.…”

Section: Discussionmentioning

confidence: 99%

Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers

Brandelli¹,

Riffel²

2005

Electro Journal of Biotech

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The strain Chryseobacterium sp. kr6 shown to be useful for biotechnological purposes such as hydrolysis of poultry feathers and de-hairing of bovine pelts. The effect of temperature, initial pH and media composition on protease production by this keratinolytic strain was studied. The enzyme was produced between 25 and 37ºC, with maximum activity and yield at 30ºC. When protease production was tested in media with different initial pH, maximum activity was observed when cultivation was carried out at 30ºC and initial pH ranging from 6.0 to 8.0. Higher activity was observed when feathers or feather meal were used as growth substrates, followed by soybean meal. The addition of carbohydrates or surfactants to feather broth resulted in decrease in keratinolytic activity.

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“…[7][8][9] The majority of psychrotrophic bacteria have the ability to form hydrolytic, thermostable enzymes that break down the major constituents of milk, such as the protein. 2 The action of such enzymes destabilises the casein in milk through hydrolysis, and the result is the formation of a gel structure or coagulation of sterilised milk during storage.…”

Section: Tablementioning

confidence: 99%

“…5 Yellow pigmented psychrotrophs included in the bacterial family Flavobacteriaceae have the potential to decompose milk and dairy products. 6 These flavobacteria produce proteolytic enzymes that may survive pasteurisation [7][8][9] and cause bitter flavours, gelation of long-life heat treated milk 10 and the production of off-odours. [11][12][13] Jooste and Britz 8 found that the practical importance of dairy flavobacteria lies as much in their psychrotrophic growth and consequent proteinase production in refrigerated milk as in their contamination of milk via poorly sanitised pipelines and equipment.…”

Section: Introductionmentioning

confidence: 99%

Spoilage potential of a novel group of bacteria isolated from dairy products

Tsôeu¹,

Jooste²,

Charimba³

et al. 2016

S. Afr. J. Sci.

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Cold-tolerant bacteria, also known as psychrotrophic bacteria, are notorious contaminants of milk in the refrigerated dairy food chain. These organisms, especially the pseudomonads, may produce heat-resistant enzymes that are responsible for the breakdown of proteins and lipids in milk and dairy products. Such reactions result in a variety of defects in the raw or unprocessed milk that may affect the suitability of such milk for further processing. The enzymes produced may cause defects in long-life dairy products such as cheese, butter and long-life milk. In the present study, a range of 18 yellow pigmented psychrotrophic bacteria, collectively known as flavobacteria, were isolated from local dairy products. One aim of this study was to identify these bacteria to species level using molecular techniques. A second aim was to determine the spoilage potential of these organisms based on profiles generated by the BIOLOG system (that may relate to hydrolytic enzymes produced). Of the 18 isolates, 14 belonged to the genus Chryseobacterium while 4 were identified as Empedobacter isolates. The most active spoilage organisms in this group were shown to be C. bovis, C. shigense and E. brevis. These findings illustrate that enzymatically catalysed defects in dairy products should not be attributed solely to acknowledged psychrotrophic bacteria such as the pseudomonads, but that flavobacterial species may also be actively involved.

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Purification and Characterization of a Metalloprotease fromChryseobacterium indologenesIx9a and Determination of the Amino Acid Specificity with Electrospray Mass Spectrometry

Cited by 32 publications

References 48 publications

Production and properties of keratinolytic proteases from three novel Gram-negative feather-degrading bacteria isolated from Brazilian soils

Production and properties of keratinolytic proteases from three novel Gram-negative feather-degrading bacteria isolated from Brazilian soils

Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers

Spoilage potential of a novel group of bacteria isolated from dairy products

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