Purification and Characterization of Acid Phosphatase from Monocrotophos (MCP) Hydrolyzing Aspergillus niger ITCC 7782.10 Isolated from Local Agricultural Field

Jain, Ruchi; Garg, Veena; Dangwal, Koushalya; Lily, Madhuri Kaushish

doi:10.5505/tjb.2013.19870

Cited by 3 publications

(1 citation statement)

References 26 publications

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“…Phosphate, *Address correspondence to this author at the Biochemistry Department, Faculty of Science-Al Faisaliah King Abdulaziz University, Jeddah, Saudi Arabia; Tel: 00966509630287; E-mail: enas_mahdy@yahoo.com however, cannot be synthesized by living organisms. Therefore, cells need to obtain phosphate from nucleic acids, and phosphorylated sugars, when Pi is limiting [8]. Thus, phosphatases are crucial enzymes for organism survival in nature as this enzyme can hydrolyze phosphate esters and provide Pi.…”

Section: Introductionmentioning

confidence: 99%

Effect of Physical and Chemical Parameters on the Activity of Purified Phosphatase Enzyme Produced by Bacillus cereus

Danial

Alkhalf²

2017

J. Biotech. Well. Indus.

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Phosphorus is one of the most important nutrients for plant growth and development. This study aimed to survey the optimum condition for phosphatase enzyme production, purified and studied its properties. Phosphatase enzyme activity was determined by end point method. Phosphatase enzyme was obtained from Bacillus cereus EME 66 isolate. The enzyme was purified and characterized, using a three-step purification procedure with 12.3-fold. The phosphatase enzyme was partially purified using ammonium sulfate fractionation followed by ultrafiltration. The acid phosphatase is a monomer protein purified gel filtration to 5.6 fold. Results showed that the optimum temperature for the purified enzyme activity was 60 °C and it was stable at temperatures below 60 °C. This enzyme was stable between pH 4.0-6.0, and the optimal pH activity was found to 5.0. The activity of the enzyme enhanced by heavy metals (Fe 3+ , Cu 2+ , Ca 2+ , Mg 2+ and K + ). The enzyme activity was strongly inhibited by heavy metals Zn 2+ . The present article reveals on enzymatic characterization of acid phosphatase enzyme.

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Section: Introductionmentioning

confidence: 99%

Effect of Physical and Chemical Parameters on the Activity of Purified Phosphatase Enzyme Produced by Bacillus cereus

Danial

Alkhalf²

2017

J. Biotech. Well. Indus.

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Toxicity and degradation of the insecticide monocrotophos

Kaur

Goyal

2019

Environ Chem Lett

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Degradation of monocrotophos by Starkeya novella YW6 isolated from paddy soil

Sun

Zhu

Yang

et al. 2015

Environ Sci Pollut Res

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A bacteria strain, YW6, capable of utilizing monocrotophos (MCP) as the sole carbon and nitrogen sources for growth was isolated from paddy soil and identified as Starkeya novella. Strain YW6 completely degraded 0.2 mM MCP within 36 h without any lag period. Addition of carbon source resulted in slowing down of the initial rate of degradation of MCP, while the presence of a more favorable source of nitrogen enhanced the degradation of MCP. In addition to the degradation of MCP, strain YW6 was also able to degrade a wide range of organophosphorus pesticides (OPs) containing P-O-C bond, but not dimethoate, which has P-S-C bond. A MCP degradation pathway was proposed on the basis of metabolite production patterns and identification of the metabolites. MCP is hydrolyzed at the P-O-C bond to form N-methylacetoacetamide and dimethyl phosphate; N-methylacetoacetamide is transformed to N-methyl-4-oxo-pentanamide, which was subsequently converted to 5-(methylamino)-5-oxo-pentanoic acid, and 5-(methylamino)-5-oxo-pentanoic acid is cleaved to glutaric acid and methylamine. These findings provide new insights into the microbial metabolism of MCP. To the best of our knowledge, this is the first report on the degradation of MCP by Starkeya bacteria.

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Purification and Characterization of Acid Phosphatase from Monocrotophos (MCP) Hydrolyzing Aspergillus niger ITCC 7782.10 Isolated from Local Agricultural Field

Cited by 3 publications

References 26 publications

Effect of Physical and Chemical Parameters on the Activity of Purified Phosphatase Enzyme Produced by Bacillus cereus

Effect of Physical and Chemical Parameters on the Activity of Purified Phosphatase Enzyme Produced by Bacillus cereus

Toxicity and degradation of the insecticide monocrotophos

Degradation of monocrotophos by Starkeya novella YW6 isolated from paddy soil

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