Purification and characterization of agrocin 84

Thompson, R.J.; Hamilton, Robert H.; Pootjes, Christine F.

doi:10.1128/aac.16.3.293

Cited by 18 publications

(12 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[4,10] Moreover, recent NMR studies showed the presence of galactofuranosyl residues with the opposite α-configuration, in Leishmania, [6] Penicillium [11] or Clostridium [12] species. In contrast to the galacto-conjugates, the nucleotide-sugar Agrocine 84, [13,14] a natural antibiotic that controls the plant cancer crown disease, [13] is notably characterized by a glucofuranose moiety whose anomeric configuration has not yet been clearly determined. Since the biosynthesis of glycoconjugates containing hexofuranosides seems to be restricted to lower organisms, it is assumed that compounds bearing such units could be strongly antigenic [4] and may be useful targets for therapy in fungal and parasitic diseases.…”

mentioning

confidence: 97%

A General and Diastereoselective Synthesis of 1,2-cis-Hexofuranosides from 1,2-trans-Thiofuranosyl Donors

Gelin

Ferrières

Plusquellec³

2000

Eur. J. Org. Chem.

View full text Add to dashboard Cite

The general formation of 1,2‐trans‐thioglycofuranosides derived from D‐galactose, D‐glucose and D‐mannose was readily accomplished starting from the corresponding alkyl glycofuranosides via per‐O‐acetyl‐hexofuranoses as key synthons. Glycosidation of ethyl or phenyl perbenzylated 1,2‐trans‐thiofuranosides afforded disaccharides containing a nonreducing 1,2‐cis‐hexofuranosyl unit, i.e. α‐D‐galactosyl, α‐D‐glucosyl or β‐D‐mannosyl, with interesting diastereoselectivities. Activation of the thiofuranosyl donors was performed by N‐iodosuccinimide and a catalytic amount of tin(II) trifluoromethanesulfonate.

show abstract

mentioning

confidence: 97%

A General and Diastereoselective Synthesis of 1,2-cis-Hexofuranosides from 1,2-trans-Thiofuranosyl Donors

Gelin

Ferrières

Plusquellec³

2000

Eur. J. Org. Chem.

View full text Add to dashboard Cite

show abstract

“…Rather, it probably is not a protein, and its molecular weight is similar to that of agrocin 84, a bacteriocin of Agrobacterium radiobacter K84 that inhibits the growth of Agrobacterium turnefaciens. Agrocin 84 is an adenine nucleotide analog with a molecular weight of 1,350 (12). However, small differs from agrocin 84 in at least two respects.…”

Section: Discussionmentioning

confidence: 99%

Bacteriocin small of fast-growing rhizobia is chloroform soluble and is not required for effective nodulation

Brussel¹,

Zaat²,

Wijffelman³

et al. 1985

J Bacteriol

View full text Add to dashboard Cite

small bacteriocin is a low-molecular-weight bacteriocin which is common in fast-growing rhizobia. As its activity could not be detected in chloroform-sterilized culture supernatants (P. R. Hirsch, J. Gen. Microbiol. 113:219-228, 1979), the bacteriocin could not be purified in order to study its mechanism of action. We report here that small is soluble in chloroform, an observation which led to effective and simple (partial) purification. Other properties of small are its low molecular weight, which is estimated to be between 700 and 1,500, its resistance to proteolytic enzymes, pectinase, and lysozyme, and its heat stability at pH 5.5 but not at pH 7.0. Its bactericidal action on exponentially growing sensitive cells was not detected until 11 h after its addition. The bactericidal action was preceded by inhibition of cell division. To determine whether smal activity is required for nodulation or nitrogen fixation, a transposon TnS-induced small-negative mutant was isolated. The observation that this strain formed normal, acetylene-reducing root nodules showed that smal production is not a prerequisite for the formation of effective nodules.

show abstract

“…radiobacter strain K84 (producer of the bacteriocin, agrocin 84), against A. radiobacter pv. tumefaciens, the cause of crown-gall disease (18,19). Application of a mutant strain which was unable to produce agrocin 84 and still reduced disease symptoms indicated that, possibly, competition with the pathogen on the host receptor site was also important (20).…”

Section: Bacterial Antibioticsmentioning

confidence: 98%

Biological Control of Plant Pathogens by Antibiotic-Producing Bacteria

Levy¹,

Carmeli²,

Inderjit³

et al. 1994

ACS Symposium Series

View full text Add to dashboard Cite

Natural biological control phenomena are very complex systems consisting of integrated biotic and abiotic factors which are influenced by seasonal changes. Mechanisms such as competition, hyperparasitism, cross protection and antibiosis, may explain the pathways of the phenomenon of biological control. Antibiosis occurs when compounds produced by certain microorganisms have harmful effects on others. Selection for antibiotic producing bacteria begins with in vitro tests but ought to be followed by in vivo experiments. Purification, identification and application of the antibiotic as compared to the application of the antagonist itself, reveals the importance of antibiosis as part of the whole antagonistic phenomenon. Isolates of biocontrol agents which lack the gene for the antibiotic and are less effective against their target pathogens, strengthen the significance of antibiosis. Unfortunately, the correlation between results of in vitro tests and of field experiments is very small. Bacteria are less resistant than other biocontrol agents (yeasts, filamentous fungi) to adverse environmental conditions such as humidity and UV radiation.Antibiosis and allelopathy consist of biochemical warfare between organisms. Allelopathy relates to the production and release of phytotoxic metabolites by plants (7) and was regarded as biochemical interactions among various types of plants (2). The term antibiosis applies to the production and release of such metabolites by microorganisms. In other studies, allelopathy and antibiosis are considered as synonyms (3, 4). The potential of this phenomenon, as part of biological control, can be used in agriculture to minimize the presence of pathogens or undesirable weeds. Cook and Baker (5) defined biological control as "the reduction of the amount of inoculum or disease-producing activity of a pathogen accomplished by or through one or more organisms other than man".A few modes of action may explain the way biological control occurs. Competition takes place when two populations simultaneously share the same environment and have similar trophic requirements (6, 7). Nutrition, space, oxygen, water and iron are the main objects for competition when limited. Hyperparasitism is

show abstract

Purification and characterization of agrocin 84

Cited by 18 publications

References 9 publications

A General and Diastereoselective Synthesis of 1,2-cis-Hexofuranosides from 1,2-trans-Thiofuranosyl Donors

A General and Diastereoselective Synthesis of 1,2-cis-Hexofuranosides from 1,2-trans-Thiofuranosyl Donors

Bacteriocin small of fast-growing rhizobia is chloroform soluble and is not required for effective nodulation

Biological Control of Plant Pathogens by Antibiotic-Producing Bacteria

Contact Info

Product

Resources

About