Purification and Characterization of an Anaerobically Induced Alanine Aminotransferase from Barley Roots

Good, Allen G.; Muench, Douglas G.

doi:10.1104/pp.99.4.1520

Cited by 67 publications

(60 citation statements)

References 26 publications

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“…GABA and succinate semialdehyde, respectively) inhibit background reactions. For example, Good and Muench (1992) demonstrated that 1 mM GABA inhibits g1utamate:pyruvate transaminase by 24%. Furthermore, accumulation of succinate semialdehyde is known to be toxic to cells (Hearl and Churchich, 1984) and may possibly inhibit other dehydrogenases.…”

Section: Resultsmentioning

confidence: 99%

Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons

Breitkreuz

Shelp

1995

Plant Physiol.

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Section: Resultsmentioning

confidence: 99%

Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons

Breitkreuz

Shelp

1995

Plant Physiol.

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“…The pyruvate enters a lactate dehydrogenase-catalyzed reaction with NADH to produce lactate and NAD + . The AlaAT activity was measured as the decrease in absorbance resulting from the consumption of NADH at 340 nm (Griffith and Vance, 1989;Good and Muench, 1992). The reaction buffer consisted of 15 mM α-oxoglutarate, 0.15 mM NADH, 0.5 M l-alanine, and 5 units of lactate dehydrogenase in 50 mM Tris-HCl buffer (pH 7.5), and the reaction was initiated by adding 100 µL of enzyme extract.…”

Section: Alanine Aminotransferase (Alaat) and Aspartate Aminotransfermentioning

confidence: 99%

The effects of NaCl stress on Jatropha cotyledon growth and nitrogen metabolism

Gao

Liu

Chung

et al. 2013

J. Soil Sci. Plant Nutr.

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The effects of treatment with 50 and 100 mM NaCl on cotyledon growth, nitrogen assimilation, and the activities of key nitrogen metabolism enzymes were investigated in developing Jatropha curcas cotyledons. Growth inhibition was observed, and relative water content in the developing cotyledons significantly decreased in the 100 mM NaCl treatment. Lower levels of NO 3 -, NO 2 -, and NH 4 + in the cotyledons were recorded when they were supplied with 50 and 100 mM NaCl. Nitrate reductase activities decline by 9.5% to 31.8% with respect to the control groups. Salt treatment also led to a marked decrease in nitrite reductase activity compared with the controls. Glutamine synthase, glutamate synthetase and dehydrogenase, alanine aminotransferase (AlaAT) and aspartate aminotransferase (AspAT) were markedly induced by the addition of NaCl. These findings will be useful for elucidating the roles key enzymes involved in nitrogen assimilation pathway of J. curcas plant.

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“…The activity of Alanine Transaminase (AlaT; EC 2.6.1.2) was quantified as described by Good and Muench (1992). The reaction mixture was 100 mmol L -1 Tris-HCl (pH 8.0), 0.2 mmol L -1 NADH, 20 mmol L -1 L-Alanine, 1 unit of Lactate dehydrogenase, 30 µL of the enzyme extract, and 10 mmol L -1 2-oxyglutarate (Start).…”

Section: Hypoxia-responsive Enzyme Activitymentioning

confidence: 99%

Physiological Responses to Hypoxia and Manganese in Eucalyptus Clones with Differential Tolerance to Vale do Rio Doce Shoot Dieback

Harguindeguy

Castro

Novais

et al. 2018

Rev. Bras. Ciênc. Solo

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ABSTRACT:Vale do Rio Doce shoot dieback (VRDSD) is an anomaly whose cause seems to be associated with hypoxic conditions and their consequences (excess Mn and Fe) triggered by elevation of the water table in areas with poor drainage. Different plants have distinct survival strategies under this form of stress. The objective of this study was to understand the physiological responses involved in the differential tolerance of eucalyptus clones to VRDSD and their relationship to hypoxia and excess Mn. A hydroponic experiment was carried out using a 2 × 2 × 2 factorial arrangement, two eucalyptus clones with different levels of tolerance to VRDSD (sensitive Urograndis hybrid -1213; and the tolerant Rio Claro hybrid -Eucalyptus grandis x unknown -2719), two concentrations of O 2 (8 and 4 mg L -1 ), and two Mn concentrations (1.39 and 300 mg L -1 ) in a randomized block design (RBD) with three replicates. Forty-day-old clones were maintained in Clark nutrient solution for 30 days. After this period, the treatments were applied for 11 days. Plant gaseous exchange shoot and root production, and the quantity of enzymes related to oxidative stress in leaves and roots were evaluated. In the tolerant clone, reactive oxygen species (ROS) were produced under hypoxic conditions, accompanied by reduction in production of dry matter, malondialdehyde (MDA), and in activity of the enzyme alcohol dehydrogenase (ADH). However, this clone had greater production of superoxide dismutase (SOD) under these conditions, an enzyme responsible for detoxification of ROS, which acts as part of the Low Oxygen Quiescence Syndrome (LOQS). In contrast, sensitive clones did not exhibit expressive reductions in growth or changes in the leaf/root ratio. These clones formed large quantities of adventitious roots and had high levels of MDA and ADH and low levels of SOD. Therefore, sensitive clones appear not to be prepared for detoxification of ROS and other toxic metabolites, but rather adopt morphological escape mechanisms, the Low Oxygen Escape Syndrome (LOES), in response to hypoxia. Thus, the period of soil waterlogging may cause the death of large numbers of roots in sensitive clones, limiting their ability to absorb water and nutrients and culminating in the death of these plants. Excess Mn seems to aggravate the damage caused by hypoxia, but it is not the causal agent of VRDSD.

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Purification and Characterization of an Anaerobically Induced Alanine Aminotransferase from Barley Roots

Cited by 67 publications

References 26 publications

Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons

Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons

The effects of NaCl stress on Jatropha cotyledon growth and nitrogen metabolism

Physiological Responses to Hypoxia and Manganese in Eucalyptus Clones with Differential Tolerance to Vale do Rio Doce Shoot Dieback

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