Purification and Characterization of an ?-Amylase Inhibitor From Maise (Zea Maize)

Blanco‐Labra, Alejandro; Iturbe-Chiñas, F. A.

doi:10.1111/j.1745-4514.1981.tb00658.x

Cited by 59 publications

(21 citation statements)

References 24 publications

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“…Protein inhibitors of serine (e.g., trypsin and chymotrypsin) and cysteine proteases have emerged as a class of antifungal proteins that have potent activity against plant and animal pathogens. Cysteine protease inhibitors have been isolated from a number of plants and form a fourth group of cystatins, the phytocystatins (10,72,86,139). The phytocystatins are single polypeptides of 10 to 12 kDa and share common structural motifs.…”

Section: Killer Proteins (Killer Toxins)mentioning

confidence: 99%

Antifungal Proteins

Selitrennikoff

2001

Appl Environ Microbiol

529

362

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Section: Killer Proteins (Killer Toxins)mentioning

confidence: 99%

Antifungal Proteins

Selitrennikoff

2001

Appl Environ Microbiol

529

362

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“…In maize, there are at least four thaumatinlike genes (Muthukrishnan et al 2001). One of these, zeamatin, can inhibit the digestive a-amylase of some insects (Blanco-Labra and Iturbe 1981). Zeamatin is represented by three oligonucleotides on the 46K microarray but none of these were differentially expressed in the current experiment.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcription profiling analyses of maize reveals candidate defensive genes for seedling resistance against corn earworm

et al. 2011

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As maize seedlings germinate into the soil, they encounter an environment teeming with insects seeking rich sources of nutrition. Maize presumably has developed a number of molecular mechanisms to ensure survival at the beginning of its life cycle. Comparative transcription analysis using microarrays was utilized to document the expression of a number of genes with potential defensive functions in seedling tissue. In addition to elevated levels of the genes involved in the biosynthesis of DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one), an anti-insect resistance molecule, other highly expressed genes in the seedling encode the following putative defensive proteins: defensin, hydroxyproline and proline-rich protein, thaumatin-like protein, lipase, cystatin, protease inhibitor, and a variety of proteases. The potential resistance genes identified occurred mainly on chromosomes 1 and 5 in the B73 genome. Analysis of promoters of seven DIMBOA biosynthetic genes identified three transcription factor binding sites that are possibly involved in regulation of the DIMBOA biosynthetic pathway. The results indicate that maize employs a wide variety of potential resistance mechanisms in seedling tissue to resist a possible insect attack.

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“…As the same time the obtained data agree with the finding of Nirmala and Muralikrishna (2003) who found that the optimum of pH α-amylases for finger millet was a range of 5.0-5.5. Mohamed et al (2009) reported that the optima of different α-amylases of wheat have broad pH optima range from 5.0 to 7.0. This pH finding of β-amylase agrees with that of Okon and Uwaifo (1984) who reported that the optimum pH of β-amylase was 5.5.…”

Section: Results and Discussion: Diastatic Power (Dp)mentioning

confidence: 99%

“…Mohamed et al(2009) reported that the activity of α-amylases increased from day 0 to 6 of germination of Triticum estivum a local Saudi Arabia wheat variety (Balady). Evans and Monday, (2009 )found that the rate of α-amylase secretion in sprouting maize, acha, rice and sorghum for 180 h is significantly high ( P≤ 0.05) with growth period .…”

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of α and β- Amylases Isolated from Millet ( Pennisetum glaucum ) Malt

Nour¹,

Yagoub²

2013

AJSIR

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ABSTRACT:The work aimed to study the diastatic power (PD) of millet malt, to purify the α and β -amylases and to characterize these enzymes. In this study the amylolytic activity initiated during the germination of millet was determined. Fourth day of germination of millet grains showed the highest of the amount of diastatic power (DP) (54.00 IOB), no significant variation (P≤ 0.05) between the extraction procedures followed either by distilled water or water with 2% peptone. DEAE-cellulose chromatography was used for the partial purification of α-and β-amylases. The results obtained from the last purification steps is 6.5o fold of α-amylase in fifth day of germination, whereas β-amylase is 37.50 fold in fourth day of germination. These amylases isolated from millet malt have interesting characteristics such as, storage stability of purified α and β-amylases at different temperature (-20 and 4ºC) for 56 days. No significant loss (P≤0.05) in the enzymes activity during the storage period for 56 days in -20ºC, whereas the loss of enzyme activity at 4ºC during the period of 56 days were 28% for α and 35% for β-amylases. The maximum activity of α-amylase was obtained at temperature 70ºC and 50ºC for β-amylase, it was clear that the α -amylase is more stable than β-amylase. The optimum pH for both α and β-amylases were 5.0 and 5.5 respectively. Therefore, these characterizations meet the prerequisites need for food industry. This work contributed with the advances in biotechnology generating of conditions for application of a new and of low price amylases source.

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Purification and Characterization of an ?-Amylase Inhibitor From Maise (Zea Maize)

Cited by 59 publications

References 24 publications

Antifungal Proteins

Antifungal Proteins

Comparative transcription profiling analyses of maize reveals candidate defensive genes for seedling resistance against corn earworm

Purification and Characterization of α and β- Amylases Isolated from Millet ( Pennisetum glaucum ) Malt

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