Purification and characterization of an extracellular β-agarase from Bacillus sp. MK03

Suzuki, Hisashi; Sawai, Yoshinori; Suzuki, Tomohiko; Kawai, Kazuhiro

doi:10.1016/s1389-1723(03)80063-4

Cited by 63 publications

(25 citation statements)

References 33 publications

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“…only three species of Bacillus i.e. Bacillus agarexedens [24], Bacillus cereus [25], and Bacillus MK03 [26,27] have been reported as agarase producers, in addition to Pseudomonas, Cytophaga, Alteromonas, Streptomyces, Vibrio, Thallasomonas, and Agarivorans to date. This is the first report on B. megaterium producing agarase.…”

Section: Resultsmentioning

confidence: 99%

“…These findings proved that apart from cultural conditions like pH, temperature, incubation time, and aeration, medium components also play a noteworthy role in the production of agarase [14]. The cultivation medium used for Bacillus MK03 also consisted of only four medium components having peptone, NaCl, agar, and beef extract resulting in specific activity of only 0.11 and 0.45 units/mg protein [26,27].…”

Section: Medium Components For Agarase Productionmentioning

confidence: 96%

“…During the process, 8.8 and 78 fold purification was achieved with specific activity of 5.81 and 51.4 units/mg protein by following only two simple steps of purification [14]. Agarase from Bacillus MK03 was purified 49.7 fold with specific activity of 22.2 units/mg protein and 129 fold with specific activity of 14.2 units/mg protein following four complex steps of purification [26,27].…”

Section: Agarase Purificationmentioning

confidence: 99%

“…An optimum temperature of 30ºC for agarase from Bacillus sp. MK03 has been reported [26,27]. Several reports where agarase retained its activity at temperatures as high as 55ºC and also in a range of 30~40ºC are available [1,2,16,18,[30][31][32].…”

Section: Effects Of Temperature and Phmentioning

confidence: 99%

“…6.6, whereas, the optimum pH for agarase reported from B. cereus was 7.0, Bacillus sp. MK03 was acidic, 6.1 as well as slightly alkaline, 7.6 [26,27]. Agarase from Alteromonas was also reported to have optimum pH of 6.5 [33].…”

Section: Effects Of Temperature and Phmentioning

confidence: 99%

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Purification, characterization and application of a novel extracellular agarase from a marine Bacillus megaterium

Khambhaty

Mody

Jha

2008

Biotechnol Bioproc E

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A marine, gram positive, aerobic, spore forming, and non flagellated bacterium which degrades low melting point (LMP) − agarose was isolated from the west coast of India and identified as _~Åáääìë=ãÉÖ~íÉêáìã based on its morphological, biochemical, and molecular characterization. This bacterium produced clear haloes or zone of clearance on agar containing plates which was a clear indication of its agarolytic property. The extracellular agarase thus obtained was purified 8.8 and 78 fold from the culture supernatant by ammonium sulfate precipitation and gel filtration, respectively. Molecular mass by gel filtration and SDS-PAGE gave values of 15 and 12 kDa, respectively. The optimum temperature and pH for maximum agarase activity were 40°C and 6.6. The activity of agarase was drastically reduced by addition of metal ions in the assay system. This agarase, gave a=h m and s max value of 4 mg/mL and 2.75 µmol/min/mg. The isolation of protoplast from agarophyte like dÉäáÇáÉää~=~ÅÉêçë~ using indegenous agarase is reported for the first time. © KSBB hÉóïçêÇëW=~Ö~ê~ëÉI=Bacillus megaterium, Gelidiella acerosa, äçï=ãÉäíáåÖ=éçáåí=~Ö~êçëÉI=êÉÇ=~äÖ~ä=éêçíçéä~ëí= = = = =

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Section: Resultsmentioning

confidence: 99%

Section: Medium Components For Agarase Productionmentioning

confidence: 96%

Section: Agarase Purificationmentioning

confidence: 99%

Section: Effects Of Temperature and Phmentioning

confidence: 99%

Section: Effects Of Temperature and Phmentioning

confidence: 99%

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Purification, characterization and application of a novel extracellular agarase from a marine Bacillus megaterium

Khambhaty

Mody

Jha

2008

Biotechnol Bioproc E

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Marine Bacteria—A Treasure House of Valuable Products and Functions

Tipre

Purohit

Dave³

2020

Marine Niche: Applications in Pharmaceutical Sciences

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Characterization of a novel β-agarase from marine Alteromonas sp. SY37–12 and its degrading products

Wang

Mou

Jiang

et al. 2005

Appl Microbiol Biotechnol

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The phenotypic and agarolytic features of an unidentified marine bacteria isolated from the southern ocean of China was studied. The strain was gram-negative, aerobic, and polarly flagellated. It was identified as the genus Alteromonas according to its morphological and physiological characterization. In solid agar, the isolate produced a diffusible agarase that caused agar softening around the colonies. An extracellular agarase was purified by the procedure of ammonium sulfate precipitation, gel filtration on Sephacryl S-100HR, and ion-exchange chromatography on diethylaminoethyl-Sepharose. The purified protein exhibited a single band on SDS-PAGE with a molecular mass of 39.5 kDa. The enzyme hydrolyzed the beta-1,4-glycosidic linkages of agar, yielding neoagarotetraose and neoagarohexaose as the main products. The optimum reaction temperature of the agarase was 35 degrees C, with a narrow range from 30 to 45 degrees C. The enzyme activity reached the maximum at pH 7.0 and in the presence of 2% NaCl. Molecular mass and degrading products showed that the agarase from Alteromonas sp. SY 37-12 was much different from those previously reported.

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Purification and characterization of an extracellular β-agarase from Bacillus sp. MK03

Cited by 63 publications

References 33 publications

Purification, characterization and application of a novel extracellular agarase from a marine Bacillus megaterium

Purification, characterization and application of a novel extracellular agarase from a marine Bacillus megaterium

Marine Bacteria—A Treasure House of Valuable Products and Functions

Characterization of a novel β-agarase from marine Alteromonas sp. SY37–12 and its degrading products

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