Purification and characterization of an extracellular chitinase from antagonistic <i>Streptomyces violaceusniger</i>

Nagpure, Anand; Gupta, Rajinder K.

doi:10.1002/jobm.201100648

Cited by 56 publications

(41 citation statements)

References 38 publications

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“…(Nawani et al, 2002), Bacillus thuringiensis , Streptomyces sp. (Han et al, 2009;Nagpure and Gupta, 2013;Jiang et al, 2012). …”

Section: Effect Of Metal Ion Addition On the Activity Of The Purifiedmentioning

confidence: 91%

“…The major inhibitor of chitinase activity was Hg 2+ since it reacts with -SH groups found in cysteine residues in the protein chain and disrupts the tertiary structure. It strongly inhibits chitinases from different genera including Bacillus (Waghmare and Ghosh, 2010), Massilia timonae (Adrangi et al, 2010), Microbispora (Nawani et al, 2002), Rhodothermus (Hobel et al, 2005), Stenotrophomonas (Jankiewicz et al, 2012) and Streptomyces (Nagpure and Gupta, 2013;Prakash et al, 2013). The other major inhibitory metal ions which show strong inhibitory action on chitinases include Fe 2+ (Pradeep et al, 2014;Wang et al, 2010), Mn 2+ (Pradeep et al, 2014;Wang et al, 2010), Ag 2+ (Nagpure and Gupta, 2013;Liu et al, 2010), Zn 2+ (Jankiewicz et al, 2012;Prakash et al, 2013) and Co 2+ (Jiang et al, 2012;Yong et al, 2005).…”

Section: Effect Of Metal Ion Addition On the Activity Of The Purifiedmentioning

confidence: 94%

“…Chitin is a high molecular weight complex polymer and is generally insoluble in aqueous media and hence its uptake as such is not possible. Hence bacteria, especially Streptomyces (Brzezinska et al, 2013;Margino et al, 2010;Nagpure and Gupta, 2013;Prakash et al, 2013;Pradeep et al, 2014) and Bacillus (Kudan and Pichyangkura, 2009;Liu et al, 2010;Natsir et al, 2010;Waghmare and Ghosh, 2010;Dai et al, 2011) species, produce extracellular chitinases for nutritional purposes, in that they are able to utilise chitin as their sole source of carbon. Application of chemical based pesticides causes long-lasting adverse effects on ecosystems and human health.…”

Section: Introductionmentioning

confidence: 96%

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RETRACTED: Purification and characterisation of an acidic and antifungal chitinase produced by a Streptomyces sp.

Karthik

Binod

Pandey

2015

Bioresource Technology

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“…(Nawani et al, 2002), Bacillus thuringiensis , Streptomyces sp. (Han et al, 2009;Nagpure and Gupta, 2013;Jiang et al, 2012). …”

Section: Effect Of Metal Ion Addition On the Activity Of The Purifiedmentioning

confidence: 91%

Section: Effect Of Metal Ion Addition On the Activity Of The Purifiedmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

RETRACTED: Purification and characterisation of an acidic and antifungal chitinase produced by a Streptomyces sp.

Karthik

Binod

Pandey

2015

Bioresource Technology

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“…Among them, we included important substrates from plant and fungal origins, such as microcrystalline cellulose (Serva), carboxymethyl cellulose (Sigma-Aldrich), xylan from beechwood (Sigma-Aldrich), starch (Sigma-Aldrich), and colloidal chitin prepared according to Nagpure and Gupta (51). The flasks were incubated at 24°C with shaking at 300 rpm for 7 days.…”

Section: Methodsmentioning

confidence: 99%

Terracidiphilus gabretensis gen. nov., sp. nov., an Abundant and Active Forest Soil Acidobacterium Important in Organic Matter Transformation

García‐Fraile

Benada

Cajthaml

et al. 2016

Appl Environ Microbiol

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dUnderstanding the activity of bacteria in coniferous forests is highly important, due to the role of these environments as a global carbon sink. In a study of the microbial biodiversity of montane coniferous forest soil in the Bohemian Forest National Park (Czech Republic), we succeeded in isolating bacterial strain S55 T , which belongs to one of the most abundant operational taxonomic units (OTUs) in active bacterial populations, according to the analysis of RNA-derived 16S rRNA amplicons. The 16S rRNA gene sequence analysis showed that the species most closely related to strain S55 T include Bryocella elongata SN10 T also reveals the presence of enzymatic machinery required for organic matter decomposition. Soil metatranscriptomic analyses found 132 genes from strain S55 T he decomposition of organic matter from plant residues in forest soils depends on microbial enzymes and environmental factors, such as temperature, moisture, pH, and soil texture. In fact, the carbon cycle in forest soils is completed principally through lignocellulose-degrading microorganisms present in the soil and guts of animals (1). The main plant cell wall polymers are polysaccharides (cellulose and hemicelluloses) and lignin. The degradation of these polymers requires the activity of extracellular enzymes (cellulases, xylanases, laccases, and peroxidases) produced from fungal and bacterial inhabitants of soil (2-6).Bacteria belonging to the phylum Acidobacteria are common in the environment. Acidobacteria are difficult to isolate and maintain in culture, but estimations based on culture-independent studies suggest that approximately 20% (7) of the bacteria in soils worldwide belong to this phylum. Particularly, in some acidic soils, members of Acidobacteria might constitute Ͼ50% of the total bacterial community (8, 9), although they can also be abundant in more-neutral soils (10-13). Thus, this phylum is as abundant in soils as is Proteobacteria. Subdivision 1 of Acidobacteria contains most of the officially described taxa within this phylum; currently, there are only 21 validated species classified in 7 genera, plus the not-yet-validated proposed new genus and species "Acidipila rosea" (14). Most of these bacteria have been isolated from acidic soils, wetlands, tundra, and peat (15)(16)(17)(18)(19)(20). The Acidobacteria phylum was originally described as including four or five subgroups or subdivisions (10, 21). As the number of available 16S rRNA gene sequences increased, the work of Barns et al. (11) expanded the known diversity encompassed within the phylum to the current 26 subdivisions. The abundance of these bacteria in soils suggests that acidobacteria play important roles in soil ecological processes. pH is the most important environmental variable affecting acidobacterial abundance (8). Besides pH, a negative correlation with organic carbon availability and total nitrogen concentration in soils was observed, suggesting an oligotrophic lifestyle of Acidobacteria (22,23). Oligotrophy and preferential colonization of bulk so...

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“…A Lineweaver-Burk plot was obtained by plotting 1/v against 1/s. Kinetic parameters (Km and Vmax) were estimated by linear regression from Lineweaver-Burk plot (Nagpure and Gupta, 2013;Rahman et al, 2014).…”

Section: Analysis Of Kinetic Parametersmentioning

confidence: 99%

Purification and molecular characterization of chitinases from soil actinomycetes

Das¹,

Kumar²,

Kumar³

et al. 2017

Afr. J. Microbiol. Res.

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Microbial extracellular chitinases are used in agriculture as effective biocontrol agents and in waste degradation, pharmaceutical and food industry. Actinomycetes are widely tapped group for production of extracellular chitinases. In the present study, approximately 260 actinomycetes were isolated from various ecological habitats was subjected to primary analyses and screened for production of chitinase by plate assay method. Diameter of zones of hydrolysis ranged from 8 to 16 mm. Based on the results, isolates 130, 194, 184, NRRLB 24916 (Streptomyces mexicanus) and NRRLB 16746 (Streptomyces albidoflavus, positive control) were selected for secondary screening and purification. Enzyme activity was estimated in crude cell free extract and partially purified samples. Activity ranged from 7.16 to 14.12 IU/ml (in crude extracts) and 12.1 to 23.10 IU/ml (in partially purified samples). In case of highest chitinase producing isolate 130, effect of various fermentation conditions (pH, temperature and substrate concentration) was studied in crude extract. Furthermore, complete purification of isolate 130 was done by column chromatography and the activity in purified fraction was found to be 32.12 IU/ml. The K m and V max values of the purified fractions for isolate 130 were 2.11 µg/ml/min and 53.11mg/ml respectively. This shows that the enzyme has high affinity for the substrate. SDS gel electrophoresis of the purified fraction showed presence of single band of approximately 65 to 70 kDa. Analyses of purified chitinase were done using MS/MS technique. N-terminal sequence corresponded to chitinase, the gene encodes a protein of 453 amino acid residues. Comparison of deduced amino acid sequence to other chitinases in the database indicated that enzyme showed 70% similarity with chitinase from Streptomyces plicatus and belongs to glycoside hydrolase family 18. Homology modeling showed that the enzyme was folded into a domain of (α/β) 8 barrel structure. Identification of secondary structure was done by CD spectroscopy. Isolate 130 was capable of degrading biodegradable wastes such as crustacean shells.

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Purification and characterization of an extracellular chitinase from antagonistic Streptomyces violaceusniger

Cited by 56 publications

References 38 publications

RETRACTED: Purification and characterisation of an acidic and antifungal chitinase produced by a Streptomyces sp.

RETRACTED: Purification and characterisation of an acidic and antifungal chitinase produced by a Streptomyces sp.

Terracidiphilus gabretensis gen. nov., sp. nov., an Abundant and Active Forest Soil Acidobacterium Important in Organic Matter Transformation

Purification and molecular characterization of chitinases from soil actinomycetes

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