Purification and Characterization of Arginase from Serum of Healthy and Type II Diabetic Iraqi Patients

Abstract

Arginase was purified from human serum of healthy and patient with type II Diabetes mellitus subjects. The purification steps included, DEAE-cellulose chromatography and gel filtration on Sepharoe6B.The molecular weight of native arginase was find to be (94 Kd), by gel filtration. The purified human serum arginase is characterized by optimum pH of (9.5), optimum pH of stability is between (9-10), optimum temperature (35 0 C), temperature of stability is between (30-50 0 C) in both healthy and patient subjects.