Purification and characterization of biologically active recombinant human Eppin expressed in Escherichia coli

Zhu, Qingyi; Gu, Xiaoling; Yang, Jin; Wang, Junhong; Tang, Bo; Wu, Hongfei

doi:10.1097/00029330-200804010-00009

Cited by 2 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So, there is an urgent need to develop harmless, retrievable products for contraception. Reproductive science has provided a range of fertility control measures for women, but the choices for men are few and currently limited to condoms and vasectomy [1]. Various health organizations and pharmaceutical companies actively continue to pursue research towards new contraceptive approaches [2].…”

Section: Introductionmentioning

confidence: 99%

Preparation, Characterization, and Determination of Immunological Activities of Transfer Factor Specific to Human Sperm Antigen

Zhou

Chen

Zhao-hong

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

Objective. The objective of this study was to prepare, characterize, and determine immunological activities of specific transfer factor (STF) specific to human sperm antigen (HSA) for the preparation of antisperm contraceptive vaccine that can be used as an immunocontraceptive. Methods. HSA-STF was prepared using the spleens of rabbits vaccinated with HSA. The specific immunological activities were examined by lymphocyte proliferation test (LPT), leukocyte adhesion inhibition test (LAIT), and by determining the concentrations of IL-4, γ-IFN, and IL-21. HSA-STF was a helveolous substance, having a pH value of 7.0 ± 0.4 and UV absorption maxima at 258 ± 6 nm. It contained seventeen amino acids; glycine and glutamic acids were the highest in terms of concentrations (38.8 μg/mL and 36.3 μg/mL, resp.). Results. The concentration of polypeptide was 2.34 ± 0.31 mg/mL, and ribose was 0.717 ± 0.043 mg/mL. The stimulation index for lymphocyte proliferation test was 1.84, and the leukocyte adhesion inhibition rate was 37.7%. There was a statistically significant difference between the cultural lymphocytes with HSA-STF and non-HSA-STF for γ-IFN and IL-21 (P < 0.05), but there was no statistical significance for IL-4 (P > 0.05). Conclusion. HSA-STF was prepared and characterized successfully. It had immunological activity which could transfer the immune response specific to HSA and prove to be a potential candidate for the development of male immunocontraceptive agents.

show abstract

Section: Introductionmentioning

confidence: 99%