Purification and Characterization of Cathepsin B from the Muscle of Horse Mackerel Trachurus japonicus

Yoshida, Asami; Ohta, Megumi; Kuwahara, Koichi; Cao, Min‐Jie; Hara, Kenji; Osatomi, Kiyoshi

doi:10.3390/md13116550

Cited by 20 publications

(10 citation statements)

References 30 publications

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“…The high activities of cathepsin B, D, and L were detected in chum salmon muscle, which was suspected to be the reason for muscle softening (Yamashita & Konagaya, 1991). Researchers have described different procedures for isolating marine cysteine or thiol proteinases from the muscle of marine fishes (Yamashita & Konagaya, ; Yoshida et al, ). However, there are few studies on proteolytic enzymes in the blood of fish.…”

Section: Resultsmentioning

confidence: 99%

Effect of blood deposition phenomenon on flesh quality of yellowtail (Seriola quinqueradiata) during storage

Jiang

Miyazaki

Hirasaka

et al. 2019

Journal of Texture Studies

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We examined the influence of blood deposition on flesh quality of ordinary muscle in yellowtail. This study compared the flesh quality changes in upper and under‐sides of yellowtails killed by two different methods: spinal‐cord destruction (SCD) with blood removal and suffocation in air without blood removal (SA). The under‐sides of the SA group showed the highest values for a*, cathepsin B and B + L activities, the lowest value in breaking strength and the greatest degradation of myosin heavy chain (MHC) among the four groups. However, the values of the SCD‐upper group indicated the best flesh quality. In addition, the white blood cells presented the highest cathepsin B and B + L activities among the blood components. These results indicate that blood has the tendency to deposit downward in accordance with the direction of placement. This phenomenon influences the distribution of white blood cells which contain enzymes that accelerate the deterioration of flesh quality. The texture of fish muscle is an important part of the flesh quality. In captured fishery (purse‐seine fishery and dragnet fishery), it is impossible to immediately and completely remove blood. Therefore, suffocation in air is the common method after the fish is caught. The commercial value of fish is decreased and the price varies greatly when these fish enter market circulation. In our study, we examined the influence of blood deposition on the flesh quality of yellowtail during storage. The degradation of structural proteins accelerated in the deposited blood which contain proteases. The movement and deposition of blood caused the difference of quality on both sides, which seriously affected the quality of fish during preservation. Our study has some theoretical guidance for muscle softening and give a better understanding of the adverse effect of blood during preservation.

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Section: Resultsmentioning

confidence: 99%

Effect of blood deposition phenomenon on flesh quality of yellowtail (Seriola quinqueradiata) during storage

Jiang

Miyazaki

Hirasaka

et al. 2019

Journal of Texture Studies

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“…We thus conclude that Jian carp CatB presumably functions in double‐chain form in vivo . The natural CatB purified from the muscle of horse mackerel ( Trachurus japonicus ) has been definitely proven to form 2 chains via the N ‐terminal sequencing of 2 bands (28 and 6 kDa) on reducing SDS‐PAGE (Yoshida and others ). However, the recombinant mature CatB obtained in the study presented only one band on reducing SDS‐PAGE and a similar phenomenon was also observed for the recombinant mature turbot (Zhou and others ).…”

Section: Resultsmentioning

confidence: 99%

Immunolocalization of Jian Carp (Cyprinus Carpio Var. Jian) Cathepsin B: Cloning, Expression, Characterization, and Antibody Preparation

Zhong

et al. 2017

Journal of Food Science

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Cathepsin B (CatB) cDNA of 759 bp from Jian carp (Cyprinus carpio var. Jian) with amino acid similarity of 99.6% to common carp was cloned. The mature CatB was expressed in Escherichia coli BL21 transferred with vector CatB-pET-30a. It was purified and identified as a single band (29 kDa) on SDS-PAGE. Optimum CatB activity was observed at 40 °C and pH 5.5. Mouse anti-CatB polyclonal antibody with a high titer of 1:256000 was prepared successfully and shown to specifically recognize the antigen both in prokaryotic cells and in the tissues of Jian carp according to western blotting and immunohistochemistry results. Immunolocation analysis showed that CatB distribution at protein level varied among the tested tissues. The results presented in this study may provide a significant reference for future research on the inherent relationship between CatB and the quality of fish or fish products at both the gene and protein levels.

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“…The scad (Decapterus maruadsi), belongs to the family of mackerel, plays an important role in Chinese marine fishing industry economics and its production in China reached 540,000 tons in 2009 (Zhong et al, 2012). In Southern China, the peak of scad harvest is during the summer.…”

Section: Introductionmentioning

confidence: 99%

“…Thiansilakul et al (2007) also studied the composition of defatted round scad protein hydrolysate with antioxidative activity. Two endogenous proteases, cathepsin L and MBSP, were isolated from blue scad skeletal muscle (Zhong et al, 2012). However, no information regarding the ironbinding peptides from scad processing byproduct hydrolysate has been reported.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Hydrolysis Conditions for the Production of Iron-Binding Peptides from Scad (<i>Decapterus maruadsi</i>) Processing Byproducts

Zhang¹,

Li²,

Zhang³

et al. 2016

American Journal of Biochemistry and Biotechnology

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The scad (Decapterus maruadsi) processing byproduct (SPB) was hydrolyzed by four commercial enzymes, namely, trypsin, flavourzyme, protamex and alcalase, for preparing high Iron-Binding Capacity (IBC) hydrolysate. Alcalase was the best choice for obtaining high IBC hydrolysate from SPB. Response surface methodology using a central-composite design was employed to optimize the enzymatic hydrolysis conditions with alcalase to obtain a maximum hydrolysate yield from the SPB with high iron-binding capacity. The best alcalase hydrolysis conditions were as following: Hydrolysis temperature of 46°C, enzyme substrate ratio of 6040 U/g-protein and hydrolysis time of 66 min, respectively. Under these optimal hydrolysis conditions, the predicted ironbinding capacity was 317.2 µg g −1 , which was consistent with the average of three replicates of 296.2 µg g −1 obtained in the validation experiments. The IBC of hydrolysate did not displayed linear relationship with antioxidative ability or the Degree of Hydrolysis (DH). Results indicated that the alcalase hydrolysate from scad processing byproduct can be developed into iron supplant ingredients in functional foods.

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Purification and Characterization of Cathepsin B from the Muscle of Horse Mackerel Trachurus japonicus

Cited by 20 publications

References 30 publications

Effect of blood deposition phenomenon on flesh quality of yellowtail (Seriola quinqueradiata) during storage

Effect of blood deposition phenomenon on flesh quality of yellowtail (Seriola quinqueradiata) during storage

Immunolocalization of Jian Carp (Cyprinus Carpio Var. Jian) Cathepsin B: Cloning, Expression, Characterization, and Antibody Preparation

Optimization of Hydrolysis Conditions for the Production of Iron-Binding Peptides from Scad (<i>Decapterus maruadsi</i>) Processing Byproducts

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