Purification and characterization of cellulase produced by Novosphingobium sp. Cm1 and its waste hydrolysis efficiency and bio-stoning potential

Abstract

The aim of the study was to purify and characterize cellulase from a previously isolated Novosphingobium sp. strain Cm1 and to evaluate its waste hydrolysis and bio-stoning efficiency. Materials and Methods:There is a growing demand for cellulase, a multipurpose enzyme widely used in industrial applications. Here, we purified cellulase from Novosphingobium sp. Cm1 by cellulose chromatography. SDS-PAGE revealed a molecular mass of 25 kDa. After 18-fold purification, the cellulase had an activity of 31.4 U/mg at pH of 5 and 40°C, and it retained activity at a wide range of pH and temperatures. The presence of Fe 2+ and Co 2+ boosted the enzyme activity by 57% and 25% respectively. The hydrolysing capacity of the strain towards cellulosic material was assessed for two paper types and the highest activity (2.6 ± 0.05 U/ml) was found with filter paper as the sole carbon source.Alterations in the structure of the papers as a result of bacterial hydrolysis were confirmed by scanning electron microscope and Fourier-transform infrared spectroscopy. The strain was also tested for its potential in various industrial applications and exhibited pectinolytic activity (6.78 ± 0.68 U/ml), xylanolytic activity (0.22 ± 0.14 U/ml) and bio-stoning ability. Conclusion:The highly active purified cellulase has a broad pH and temperature range. The strain possesses waste-hydrolysing ability, pectinolytic and xylanolytic ability along with bio-stoning capacity. Significance and Impact of the Study:The efficacy and versatility of the enzyme from Novosphingobium sp. Cm1 make it an excellent candidate for diverse industrial applications.

Metagenomic insights into the development of microbial communities of straw and leaf composts

“…The observations of dynamics at the taxonomic level are consistent with the earlier findings, specifically the prevalence of Pseudomonadota during the initial stages of straw colonization from soil and the diversification of the community in the later phases (8,39). Genera detected by Illumina sequencing in both types of composts -Pseudomonas, Bacillus, Flavobacterium, Sphingobacterium, and Novosphingobium -were reported multiple times to contain potentially active cellulolytic species (77)(78)(79)(80). Cellulose-degrading strains from Pseudoxanthomonas suwonensis (81) and Luteimonas (82) were isolated from the soil.…”

Characterization of an endo‐beta‐1,4 glucanase gene from paper‐degrading and denim bio‐stoning cellulase producing Aspergillus isolates

“…Bio‐stoning process was conducted using a sustainable and environmentally friendly process as described previously 42 . Indigo dyed denim fabric was collected from textile mills in Bangladesh before any washing treatment.…”

“…Bio-stoning process was conducted using a sustainable and environmentally friendly process as described previously. 42 Indigo dyed denim fabric was collected from textile mills in Bangladesh before any washing treatment. For bio-stoning, treatment of denim cloth (3 × 3 cm) was done with 25 ml crude cellulase (∼60 U) in a 250-ml Erlenmeyer flask with 10 steel balls of 0.7 cm diameter.…”