Purification and Characterization of Cellulase from Obligate Halophilic Aspergillus flavus (TISTR 3637) and Its Prospects for Bioethanol Production

Bano, Amna; Chen, Xiaoming; Prasongsuk, Sehanat; Akbar, Ali; Lotrakul, Pongtharin; Punnapayak, Hunsa; Anwar, Muhammad; Sajid, Sumbal; Imran, Ali

doi:10.1007/s12010-019-03086-y

Cited by 39 publications

(10 citation statements)

References 21 publications

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“…Cellulase is an enzyme complex system, which consists of exoglucanase, endoglucanase and β-glucosidase, that hydrolyses the β-1,4 linkages of the cellulose chain into glucose (Kuhad et al 2016). Cellulases are widely used such as in the food, fuel, textiles, pulp and paper industries (Bhat 2000;Bano et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Lignocellulose hydrolytic enzymes production byAspergillus flavusKUB2 using submerged fermentation of sugarcane bagasse waste

Namnuch

Thammasittirong

2020

Mycology

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Lignocellulosic wastes, rice straw, sugarcane bagasse, rice bran and sawdust, and pure commercial carboxymethyl cellulose (CMC) and xylan were used as substrates to cultivate cellulolytic fungus, Aspergillus flavus KUB2, in submerged fermentation at 30°C. Of all the substrates, sugarcane bagasse was a good source for the production of cellulolytic and also hemicellulolytic enzymes. The maximum activities of endoglucanase (CMCase), total cellulase (FPase) and xylanase using sugarcane bagasse as substrate were 8%, 75% and 165%, respectively, higher than those of the commercial substrates. The time course determination of enzyme production revealed that the highest CMCase (1.27 U/ml), FPase (0.72 U/ml) and xylanase (376.81 U/ml) activities were observed at 14 days of fermentation. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) analyses confirmed the efficient structural alteration of sugarcane bagasse caused by enzymatic actions during A. flavus KUB2 cultivation. Based on the results of the hydrolytic enzyme activities, FTIR and SEM, A. flavus KUB2 is suggested as an efficient hydrolytic enzymes producer and an effective lignocellulose degrader, while sugarcane bagasse can be applied as a low-cost carbon source for the economical production of lignocellulose hydrolytic enzymes by A. flavus KUB2.

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Section: Introductionmentioning

confidence: 99%

Lignocellulose hydrolytic enzymes production byAspergillus flavusKUB2 using submerged fermentation of sugarcane bagasse waste

Namnuch

Thammasittirong

2020

Mycology

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“…The efficiency of protease depends on several factors such as ionic strength, pH of detergent, bleaching agent, washing temperature, mechanical handling and composition of the detergent. Although high yielding alkaline protease strains have been widely studied, but bleach-stable enzymes are still not available where only few studies have been reported (Mei and Jiang, 2005;Srinubabu et al, 2007;Lakshmi et al, 2014;Ochi et al, 2017;Bano et al, 2019). Due to their broad specificity, numerous alkaline proteases have been identified and purified from many Bacilli strains (Bhaskar et al, 2007;Doddapaneni et al, 2007;Muhammad et al, 2019;Osire et al, 2019), such as subtilisin, which has been produced by Bacillus licheniformis (Jacobs 1985) and Bacillus amyloliquefaciens (Wells et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Geobacillus stearothermophilus protease for detergent industry

Iqbal¹,

Aftab²,

Afzal³

et al. 2020

Rev.Mex.Ing.Quim.

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“…Cellulolytic microorganisms play an important role in the biosphere by recycling cellulose, the most abundant carbohydrate produced by plants 29 . Cellulases are widely used such as in the food, fuel, textiles, pulp and paper industries 30,31 .…”

Section: Resultsmentioning

confidence: 99%

Isolation and Screening of Fungal Culture Isolated From Algerian Soil for the Production of Cellulase and Xylanase

Gahfif¹,

Souagui²,

Azzouz³

et al. 2020

J. Drug Delivery Ther.

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Lignocellulolytic enzymes constitute a very large group of extracellular proteins secreting by fungi who is ecologically involved in the degradation of a variety of complex materials, a property that is attributed to a battery of enzymes produced by these microorganisms like cellulases and xylanases who are of significant industrial value and relevance. Forty fungal isolated from rich soil in organic matter were screened for lignocellulolytic enzymes production, its organized on the basis of their hydrolytic potential of cellulose and xylan. The isolates strains presented enzymatic activity which was ranked as follows: cellulolytic (56%), xylanolytic (44%). Some selected strains that produce high levels of enzymes (cellulase, xylanase) grown in submerged fermentation (SmF) and were quantitatively evaluated. The fermentation experiments were carried out in shake flasks. The highest CMCase (5,10 IU/ml) and xylanase (98,25 IU/ml) activities were obtained from Trichoderma sp strain Mtr6 isolate. Keywords: Fungi, Trichoderma sp, lignocellulolytic enzymes, soil, screening, organic matter.

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Purification and Characterization of Cellulase from Obligate Halophilic Aspergillus flavus (TISTR 3637) and Its Prospects for Bioethanol Production

Cited by 39 publications

References 21 publications

Lignocellulose hydrolytic enzymes production byAspergillus flavusKUB2 using submerged fermentation of sugarcane bagasse waste

Lignocellulose hydrolytic enzymes production byAspergillus flavusKUB2 using submerged fermentation of sugarcane bagasse waste

Characterization of Geobacillus stearothermophilus protease for detergent industry

Isolation and Screening of Fungal Culture Isolated From Algerian Soil for the Production of Cellulase and Xylanase

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