Purification and Characterization of Chitinases from Ridgetail White Prawn Exopalaemon carinicauda

Wang, Jing; Zhang, Jiquan; Song, Fengge; Gui, Tianshu; Xiang, Jianhai

doi:10.3390/molecules20021955

Cited by 28 publications

(16 citation statements)

References 37 publications

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“…90 The chitinolytic genes and enzymes have been identified in a wide range of insects [104][105][106][107][108][109][110][111][112][113][114][115][116][117][118] and crustacean species, except for Allotriocarida. [119][120][121][122][123][124][125][126][127][128][129][130][131][132][133][134][135][136][137] The activities of chitinolytic enzymes positively rely on the rising 20E titer, with chitobiase being induced earlier at relatively lower 20E level and chitinases being induced when the 20E level peaks. 106,138 The EcREs have not been identified in the sequences of genes encoding the chitinolytic enzymes, 90 indicating the possibility of indirect transcriptional regulation by EcR.…”

Section:  Neuroendocrine Regulation Of Moltingmentioning

confidence: 99%

Ecdysone Receptor Agonism Leading to Lethal Molting Disruption in Arthropods: Review and Adverse Outcome Pathway Development

Shang

Villeneuve

Toyota

et al. 2017

Environ. Sci. Technol.

119

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Molting is critical for growth, development, reproduction, and survival in arthropods. Complex neuroendocrine pathways are involved in the regulation of molting and may potentially become targets of environmental endocrine disrupting chemicals (EDCs). Based on several known ED mechanisms, a wide range of pesticides has been developed to combat unwanted organisms in food production activities such as agriculture and aquaculture. Meanwhile, these chemicals may also pose hazards to nontarget species by causing molting defects, and thus potentially affecting the health of the ecosystems. The present review summarizes the available knowledge on molting-related endocrine regulation and chemically mediated disruption in arthropods (with special focus on insects and crustaceans), to identify research gaps and develop a mechanistic model for assessing environmental hazards of these compounds. Based on the review, multiple targets of EDCs in the molting processes were identified and the link between mode of action (MoA) and adverse effects characterized to inform future studies. An adverse outcome pathway (AOP) describing ecdysone receptor agonism leading to incomplete ecdysis associated mortality was developed according to the OECD guideline and subjected to weight of evidence considerations by evolved Bradford Hill Criteria. This review proposes the first invertebrate ED AOP and may serve as a knowledge foundation for future environmental studies and AOP development.

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Section:  Neuroendocrine Regulation Of Moltingmentioning

confidence: 99%

Ecdysone Receptor Agonism Leading to Lethal Molting Disruption in Arthropods: Review and Adverse Outcome Pathway Development

Shang

Villeneuve

Toyota

et al. 2017

Environ. Sci. Technol.

119

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show abstract

“…In our previous research, we purified and characterized two kinds of chitinase (EcChi1 and EcChi2) from the hepatopancreas of Exopalaemon carinicauda (Wang et al 2015), which is one of the most important commercial shrimp in China (Li et al 2012). According to the transcriptome data of E. carinicauda , the full-length cDNA sequences of EcChi1 and EcChi2 were obtained (Wang et al 2015).…”

mentioning

confidence: 99%

“…According to the transcriptome data of E. carinicauda , the full-length cDNA sequences of EcChi1 and EcChi2 were obtained (Wang et al 2015). …”

mentioning

confidence: 99%

CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda

Gui

Zhang

Song

et al. 2016

G3 Genes|Genomes|Genetics

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The development of the type II clustered regularly interspaced short palindromic repeats (CRISPR) system has resulted in the revolution of genetic engineering, and this technology has been applied in the genome editing of various species. However, there are no reports about target-specific genome editing in shrimp. In this research, we developed a microinjection method for the ridgetail white prawn Exopalaemon carinicauda and successfully applied CRISPR/Cas9 technology to the genome editing of E. carinicauda. Through coinjection of mRNA of Cas9 nuclease and gRNA specialized for E. carinicauda chitinase 4 (EcChi4), shrimps with indel mutations were obtained. Further analysis showed that the mutations could be transmitted to the next generation. This is the first time that site-specific genome editing has been successfully demonstrated in a decapod, and will further contribute to the study of functional genomics in decapods.

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“…Denaturasi ini menyebabkan perubahan pada konformasi enzim akibat adanya perenggangan ikatan hidrogen yang bersifat reversibel sehingga dapat mempengaruhi sisi aktif enzim untuk berikatan dengan substrat (Haedar et al 2017). Penelitian Wang et al (2015) melaporkan bahwa kisaran suhu optimal untuk aktivitas kitinase kisaran 40 -85ºC.…”

Section: Pengaruh Suhuunclassified

KARAKTERISTIK DAN SIFAT KINETIKA ENZIM KITINASE ASAL JAMUR ENTOMOPATOGEN <i>Beauveria bassiana<i/>

Elawati

Pujiyanto

Kusdiyantini

2018

J Bioteknol Biosains Indones

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Characteristics and Kinetics of Chitinase Enzyme from Entomopathogenic Fungus Beauveria bassianaBeauveria bassiana is one of the entomopathogenic fungi that produces chitinase when infecting the host. Chitinase is widely used as biocontrol agents because it can degrade chitin into an environmentally friendly product. This study aims to characterize and test the kinetics of chitinase from B. bassiana. This characterization includes determination of pH and optimum temperature, enzyme stability and enzyme kinetics test by determining Km and Vmax value with Lineweaver-Burk equations. The result of experiment showed that the chitinase B. bassiana had pH and optimum temperature of 5 and 40ºC respectively. This enzyme was stable until 90 minutes incubation at 40ºC. The Km and Vmax values were 0.181 mg/L and 0.022 mg/L.sec respectively. The Km value is higher than Vmax, which means the affinity of the enzyme to the lower substrate requiring high substrate concentration to increase the reaction rate. It can be concluded that the chitinase activity of B. bassiana is still low.Keywords: Beauveria bassiana, characteristics and kinetics, chitinase enzyme, entomopathogenic, Lineweaver-BurkABSTRAKBeauveria bassiana merupakan salah satu jamur entomopatogen yang memproduksi kitinase saat menginfeksi inangnya. Enzim kitinase saat ini banyak digunakan sebagai agen biokontrol karena dapat mendegradasi kitin menjadi produk yang ramah lingkungan. Penelitian ini bertujuan untuk mengkarakterisasi dan menguji kinetika enzim kitinase asal jamur B. bassiana. Metode yang digunakan dalam karakterisasi ini mencakup penentuan pH dan suhu optimum, kestabilan enzim pada suhu optimumnya, dan uji kinetika enzim yang mencakup penentuan nilai Km dan Vmaks dengan persamaan Lineweaver-Burk. Hasil penelitian karakterisasi menunjukkan bahwa enzim kitinase B. bassiana mempunyai pH dan suhu optimum masing-masing 5 dan 40ºC. Enzim ini stabil sampai pada 90 menit inkubasi pada suhu 40ºC. Nilai Km diperoleh 0,181 mg/L dan Vmaks sebesar 0,022 mg/L.detik. Nilai Km lebih tinggi daripada Vmaks, yang artinya afinitas enzim terhadap substrat rendah sehingga membutuhkan konsentrasi substrat yang tinggi untuk meningkatkan kecepatan reaksi, maka dapat disimpulkan bahwa aktivitas kitinase dari B. bassiana masih tergolong rendah.Kata kunci: Beauveria bassiana, entomopatogen, enzim kitinase, karakteristik dan kinetik, Lineweaver-Burk

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Purification and Characterization of Chitinases from Ridgetail White Prawn Exopalaemon carinicauda

Cited by 28 publications

References 37 publications

Ecdysone Receptor Agonism Leading to Lethal Molting Disruption in Arthropods: Review and Adverse Outcome Pathway Development

Ecdysone Receptor Agonism Leading to Lethal Molting Disruption in Arthropods: Review and Adverse Outcome Pathway Development

CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda

KARAKTERISTIK DAN SIFAT KINETIKA ENZIM KITINASE ASAL JAMUR ENTOMOPATOGEN <i>Beauveria bassiana<i/>

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