Purification and characterization of Cu,Zn superoxide dismutase from pumpkin (Cucurbita moschata) pulp

Xiao-rong, Qin; Zhang, Mingjin

doi:10.1007/s00217-012-1829-1

Cited by 9 publications

(2 citation statements)

References 20 publications

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“…But rPsSOD had poor thermal stability and retained approximately 59.4% residual enzyme activity after incubation at 50°C for 40 min, while a sharply decrease on activity at 60 and 70°C (Fig.5B). In contrast, pumpkin pulp Cu/Zn-SOD retained more than 80% of its original activity at 55°C for 40 min, or even remained approximately 45% of its original activity at 65°C for 40 min [28], and the recombinant Geobacillus sp. SOD retained about 55% residual activity after treatment at 90°C for 1 h [19].…”

Section: Discussionmentioning

confidence: 92%

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Cloning, expression and biochemical characterization of recombinant superoxide dismutase from Antarctic psychrophilic bacterium Pseudoalteromonas sp. ANT506

Wang

Hou

et al. 2015

J. Basic Microbiol.

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In this study, a superoxide dismutase gene (PsSOD) from Pseudoalteromonas sp. ANT506 was cloned and over expressed in Escherichia coli. The PsSOD has an open reading frame of 582 bp with a putative product of 193 amino acid residue and an estimated molecular size of 21.4 kDa. His-tagged PsSOD was subsequently purified 12.6-fold by Ni-affinity chromatography and the yield of 22.9%. The characterization of the purified rPsSOD exhibited maximum activity at 30 °C and pH 8.0. The enzyme exhibited 13.9% activity at 0 °C and had high-thermo lability at higher than 50 °C. rPsSOD exhibited well capability to 2.5 M NaCl (62.4%). These results indicated that rPsSOD exhibited special catalytic properties.

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Section: Discussionmentioning

confidence: 92%

“…B). In contrast, pumpkin pulp Cu/Zn‐SOD retained more than 80% of its original activity at 55 °C for 40 min, or even remained approximately 45% of its original activity at 65 °C for 40 min , and the recombinant Geobacillus sp. SOD retained about 55% residual activity after treatment at 90 °C for 1 h .…”

Section: Discussionmentioning

confidence: 95%