Purification and characterization of cysteine rich-secretory proteins (CRiSPs) from the venom of the Southern Pacific rattlesnake (Crotalus oreganus helleri): Their role on blood and lymphatic endothelial cell permeability

Marquez, Jessenia; Parra, Victoria; Suntravat, Montamas; Sánchez, Elda E.

doi:10.1016/j.toxicon.2018.06.017

Cited by 2 publications

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“…Cysteine-rich secretory proteins (CRiSPs) are a protein superfamily that has gained attention as a potential biopharmaceutical agent. CRiSPs are widely distributed in both genera of rattlesnakes [ 44 , 52 , 109 , 131 , 132 , 133 ] and have been crystalized and studied from snake venoms [ 134 ]. Members are single-chain peptides that contain approximately 230 amino acid residues [ 124 , 134 ], weigh 20–30 kDa [ 60 , 135 ], and contain a consistent pattern of 16 cysteines that are participating in internal disulfide linkages; hence the family name [ 60 , 134 ].…”

Section: Venom Constituents and Biomedical Applicationsmentioning

confidence: 99%

“…These interactions with different channels have been studied but require additional investigation [ 138 , 139 ]. Other intriguing activities from CRiSPs are anti-angiogenic activities [ 124 ], antiprotozoal activities (against Trypanosomes and Leishmania ) [ 60 ], involvement within the inflammatory processes [ 138 ], and the inhibition of human umbilical vascular endothelial cell proliferation [ 131 ]. They appear to be non-toxic for mice and insects [ 60 , 124 ].…”

Section: Venom Constituents and Biomedical Applicationsmentioning

confidence: 99%

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A Review of Rattlesnake Venoms

Phan,

Deshwal,

McMahon

et al. 2023

Toxins

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Venom components are invaluable in biomedical research owing to their specificity and potency. Many of these components exist in two genera of rattlesnakes, Crotalus and Sistrurus, with high toxicity and proteolytic activity variation. This review focuses on venom components within rattlesnakes, and offers a comparison and itemized list of factors dictating venom composition, as well as presenting their known characteristics, activities, and significant applications in biosciences. There are 64 families and subfamilies of proteins present in Crotalus and Sistrurus venom. Snake venom serine proteases (SVSP), snake venom metalloproteases (SVMP), and phospholipases A2 (PLA2) are the standard components in Crotalus and Sistrurus venom. Through this review, we highlight gaps in the knowledge of rattlesnake venom; there needs to be more information on the venom composition of three Crotalus species and one Sistrurus subspecies. We discuss the activity and importance of both major and minor components in biomedical research and drug development.

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Section: Venom Constituents and Biomedical Applicationsmentioning

confidence: 99%

Section: Venom Constituents and Biomedical Applicationsmentioning

confidence: 99%

A Review of Rattlesnake Venoms

Phan,

Deshwal,

McMahon

et al. 2023

Toxins

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A Meta-Analysis of the Protein Components in Rattlesnake Venom

Deshwal

Phan

Datta

et al. 2021

Toxins

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The specificity and potency of venom components give them a unique advantage in developing various pharmaceutical drugs. Though venom is a cocktail of proteins, rarely are the synergy and association between various venom components studied. Understanding the relationship between various components of venom is critical in medical research. Using meta-analysis, we observed underlying patterns and associations in the appearance of the toxin families. For Crotalus, Dis has the most associations with the following toxins: PDE; BPP; CRL; CRiSP; LAAO; SVMP P-I and LAAO; SVMP P-III and LAAO. In Sistrurus venom, CTL and NGF have the most associations. These associations can predict the presence of proteins in novel venom and understand synergies between venom components for enhanced bioactivity. Using this approach, the need to revisit the classification of proteins as major components or minor components is highlighted. The revised classification of venom components is based on ubiquity, bioactivity, the number of associations, and synergies. The revised classification can be expected to trigger increased research on venom components, such as NGF, which have high biomedical significance. Using hierarchical clustering, we observed that the genera’s venom compositions were similar, based on functional characteristics rather than phylogenetic relationships.

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Purification and characterization of cysteine rich-secretory proteins (CRiSPs) from the venom of the Southern Pacific rattlesnake (Crotalus oreganus helleri): Their role on blood and lymphatic endothelial cell permeability

Cited by 2 publications

References 0 publications

A Review of Rattlesnake Venoms

A Review of Rattlesnake Venoms

A Meta-Analysis of the Protein Components in Rattlesnake Venom

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