Purification and Characterization of Esterase 1F, the Albumin Esterase of the House Mouse (Mus musculus)

Otto, Johannes; Ronai, Adam; Deimling, Otto von

doi:10.1111/j.1432-1033.1981.tb05331.x

Cited by 35 publications

(17 citation statements)

References 25 publications

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“…5 ) which have previously been shown to be immunologically related [ 5 ] . However, esterase 6A does not cross-react with serum raised against esterase 2B (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…However, esterase 6A does not cross-react with serum raised against esterase 2B (Fig. 5 ) ; similarly neither esterase 1F nor esterase 9A precipitate with serum raised against esterase 2B [5]. All foul-esterases are coded for by the so-called esterase region [2Y] on chroniosome 8 and the gene for esterase 6 was located between the genes for esterases 1 and 2 [30].…”

Section: Discussionmentioning

confidence: 99%

“…The median values of K, and kcA, were located to yield the hest cstiiiiation of these piiraiiictcrs Table 2. Characteristic genetic and biochemical properties of esterasc l F , 9 A and 6A3 Data are taken from [2] and [5]. Kinetic constants K , and k,,, refer .0, has been described in the rat liver [23].…”

Section: Discussionmentioning

confidence: 99%

“…The procedure was repeated after 6 weeks and the animals were bled after 3 months. Antibodies, were concentrated by ammonium sulphate precipitation and stored in phosphate buffer (0.1 mol/l, pH 7.3, 0.9%, w/v, sodium chloride) at -70 "C. Antisera against esterase IF, 2B and 9A were prepared as described previously [2,4,5]. Double-diffusion experiments were performed according to Ouchterlony [16].…”

Section: Immunological Proceduresmentioning

confidence: 99%

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Purification and Characterization of Esterase 6A, a Trimeric Esterase of the House Mouse (Mus musculus)

Oehm

Looze

Ronai

et al. 1982

European Journal of Biochemistry

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Esterase 6A was isolated from mouse lung and purified 440-fold by ion-exchange chromatography, inverse ammonium sulphate gradient solubilization, gel filtration and isoelectric focusing. The resultant product was apparently homogenous by the criteria of polyacrylamide gel electrophoresis and immunodiffusion, and consisted of the electrophoretic form 6A3. A single species of subunit was present on sodium dodecyl sulphate gel electrophoresis. The molecular weight of the native protein was found to be about 178000 with a subunit molecular weight of about 60000. The equivalent weight obtained by active-site titration with diethyl-p-nitrophenyl phosphate was approximately 178 000 g/mol, indicating a functional asymmetry in the trimer. The enzyme was shown to have a high affinity for 4-nitrophenyl hexanoate (Michaelis constant K , = 4.4 pmol/l) with a relatively low catalytic efficiency (catalytic constant k,,, = 12 s-'). Esterase 6A was immunologically related to esterase 1 and esterase 9, with which it is genetically closely linked. Further properties of the three esterases were compared.

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“…5 ) which have previously been shown to be immunologically related [ 5 ] . However, esterase 6A does not cross-react with serum raised against esterase 2B (Fig.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Immunological Proceduresmentioning

confidence: 99%

See 2 more Smart Citations

Purification and Characterization of Esterase 6A, a Trimeric Esterase of the House Mouse (Mus musculus)

Oehm

Looze

Ronai

et al. 1982

European Journal of Biochemistry

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“…1. 1) from animal sources have been carried out, and it has been reported that plural esterases are present in liver/,2) serum 3 ) or other tissues,4,S) as so-called isoenzymes. Recently, characterization of individualesterases belonging to isoenzymes from animal sources has been attempted, to clarify the mechanism of formation of complicated isoenzymes.…”

mentioning

confidence: 99%

Properties and Substrate Specificities of Four Esterases fromAspergillus nigerNRRL 337

Okumura

Iwai

Tsujisaka

1983

Agricultural and Biological Chemistry

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Micro two‐dimensional gel electrophoresis of serum and testis esterases from different strains of mice

Abou-Haïla

1990

Electrophoresis

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Two-dimensional electrophoresis with time-dependent polyacrylamide gradient gel electrophoresis (PAGGE) in the second dimension was applied to the separation of native molecular forms of esterases from serum and testis of four strains of mice (C57BL/6J, Swiss OF1, F1 hybrid derived from these two populations and Tfm). In Phast System, a modified pH 3-9 gradient, a linear 8-25% gel gradient and a migration time corresponding to 300 Vh, were found to provide the best conditions for esterase analysis. About 70 esterase-active fractions could be separated with good reproducibility. The variants were characterized by their pI (3.9-7.35), their relative mobility and the visual estimation of their susceptibility towards neuraminidase and different esterase inhibitors. In the two tissues, the distribution of the esterase variants corresponded to a 50-500 kDa molecular mass range of calibration proteins, but most of the serum and testis-specific isoforms were confined to the 59-72 kDa range. All serum variants contained a terminal N-acetylneuraminic acid residue, whereas only the testicular esterases in common with those in serum appeared sensitive to neuraminidase. Cholinesterases with a low relative mobility and carboxylesterases with a high relative mobility were detected in serum, while carboxylesterases accounted for the greatest part in the testis which also contained cholinesterases and acetylesterases. Minor interspecies differences were found between C57BL/6J and Swiss OF1 esterases. The expression of two variants which differed between these two species seemed intermediate for the hybrid originating from these two populations. Two new spots were detected in the two-dimensional map of esterases from the strain bearing the Tfm mutation.

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Purification and Characterization of Esterase 1F, the Albumin Esterase of the House Mouse (Mus musculus)

Cited by 35 publications

References 25 publications

Purification and Characterization of Esterase 6A, a Trimeric Esterase of the House Mouse (Mus musculus)

Purification and Characterization of Esterase 6A, a Trimeric Esterase of the House Mouse (Mus musculus)

Properties and Substrate Specificities of Four Esterases fromAspergillus nigerNRRL 337

Micro two‐dimensional gel electrophoresis of serum and testis esterases from different strains of mice

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