Purification and Characterization of FBI-1, a Cellular Factor That Binds to the Human Immunodeficiency Virus Type 1 Inducer of Short Transcripts

Peßler, Frank; Pendergrast, P. Shannon; Hernandez, Nouria

doi:10.1128/mcb.17.7.3786

Cited by 71 publications

(85 citation statements)

References 68 publications

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“…comm.). Another transcriptional activity that could compete with Tat is the DNA-binding activity associated with the IST (inducer of short transcripts), which stimulates attenuated transcripts without affecting the synthesis of long transcripts (Pessler et al 1997). The identification of these various negative effectors of elongation will be critical for a complete understanding of the role of PTEFb (TAK) and Tat in transcriptional regulation.…”

Section: Implications For the Mechanism Of Tat Activation And Tar Rnamentioning

confidence: 99%

Taking a new TAK on Tat transactivation

1997

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Section: Implications For the Mechanism Of Tat Activation And Tar Rnamentioning

confidence: 99%

Taking a new TAK on Tat transactivation

1997

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“…ZBTB7A, also known as Pokemon, FBI [1] or LRF [2], is a transcriptional factor that belongs to the POK protein family. The ZBTB7A gene has been shown to play roles in adipogenesis [3], T-lymphoid lineage differentiation, terminal preadipocyte differentiation promotion [2] and carcinogenesis [4,5].…”

Section: Introductionmentioning

confidence: 99%

Global mapping of ZBTB7A transcription factor binding sites in HepG2 cells

Sun

et al. 2010

Cellular and Molecular Biology Letters

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ZBTB7A is a known proto-oncogene that is implicated in carcinogenesis and cell differentiation and development. Fully understanding the function of ZBTB7A in cellular processes could provide useful strategies for cancer treatment and development-associated disease therapy. Here, global mapping of ZBTB7A transcription factor binding sites was developed by utilizing microarray technology in HepG2 cells. The data obtained from the microarrays was further validated via chromatin immunoprecipitation-PCR (ChIP-PCR) and real time-PCR, and it was revealed that ZBTB7A may be one of the regulators of neural development. ZBTB7A target signal pathways were identified in signal pathway and GO (Gene Ontology) analyses. This is the first report on the global mapping of ZBTB7A downstream direct targets, and these findings will be useful in understanding the roles of ZBTB7A in cellular processes.

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“…In addition, a significant overlap between cyclin T1 and nuclear speckles was observed. However, although Cdk9 was present in the vicinity of nuclear speckles, the degree of overlap was limited (Herrmann et al 2001;Matera et al 1993).Further evidence for a link between transcription and speckles comes from observations on FBI-1, a cellular POZ-domain-containing protein that binds to the HIV-1 long terminal repeat and associates with the HIV-1 transactivator protein Tat (Pessler et al 1997). FBI-1 has been found to partially colocalize with Tat and its cellular cofactor, P-TEFb at nuclear speckles (Pendergrast et al 2002).…”

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confidence: 99%

Nuclear Speckles

Spector¹,

Lamond²

2010

Cold Spring Harbor Perspectives in Biology

752

720

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Nuclear speckles, also known as interchromatin granule clusters, are nuclear domains enriched in pre-mRNA splicing factors, located in the interchromatin regions of the nucleoplasm of mammalian cells. When observed by immunofluorescence microscopy, they usually appear as 20-50 irregularly shaped structures that vary in size. Speckles are dynamic structures, and their constituents can exchange continuously with the nucleoplasm and other nuclear locations, including active transcription sites. Studies on the composition, structure, and dynamics of speckles have provided an important paradigm for understanding the functional organization of the nucleus and the dynamics of the gene expression machinery.T he mammalian cell nucleus is a highly compartmentalized yet extremely dynamic organelle (reviewed in Misteli 2001a;Spector 2006;Zhao et al. 2009). Many nuclear factors are localized in distinct structures, such as speckles, paraspeckles, nucleoli, Cajal bodies, polycomb bodies, and promyelocytic leukemia bodies and show punctate staining patterns when analyzed by indirect immunofluorescence microscopy (reviewed in Lamond et al. 1998;Spector 2001;Spector 2006).In mammalian cells the pre-mRNA splicing machinery, including small nuclear ribonucleoprotein particles (snRNPs), spliceosome subunits, and other non-snRNP protein splicing factors, shows a punctate nuclear localization pattern that is usually termed "a speckled pattern" but has also been referred to as "SC35 domains (Wansink et al. 1993)" or "splicing factor compartments (Phair et al. 2000)" (Figs. 1 and 2). The first detailed description of the nuclear domains that we presently refer to as nuclear speckles was reported by Santiago Ramó n y Cajal in 1910 (Ramó n y Cajal 1910; reviewed in Lafarga et al. 2009). Ramó n y Cajal used acid aniline stains to identify structures he referred to as "grumos hialinas" (literally "translucent clumps"). The term "speckles" was first put forth in 1961 by J. Swanson Beck (Beck 1961) upon examination of rat liver sections immunolabeled with the serum of individuals with autoimmune disorders. Although the connection was not made at the time, these speckles had been identified two years earlier by Hewson Swift (Swift 1959) at the electron microscopic level and called interchromatin particles. Swift observed that these particles were not randomly distributed but that they occurred in localized "clouds," and cytochemical analysis indicated that they contained RNA (Swift 1959). However, the first link between pre-mRNA splicing and nuclear speckles or interchromatin granule clusters came from an examination of the distribution of snRNPs using anti-splicing factor-specific antibodies, demonstrating a speckled distribution pattern of snRNPs in cell nuclei (Lerner et al. 1981;Perraud et al. 1979;Spector et al. 1983).It is now clear that much of the punctate localization of splicing factors observed by immunofluorescence microscopy corresponds to the presence of these factors in nuclear speckles of variable size and irregular shape that...

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Purification and Characterization of FBI-1, a Cellular Factor That Binds to the Human Immunodeficiency Virus Type 1 Inducer of Short Transcripts

Cited by 71 publications

References 68 publications

Taking a new TAK on Tat transactivation

Taking a new TAK on Tat transactivation

Global mapping of ZBTB7A transcription factor binding sites in HepG2 cells

Nuclear Speckles

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