Purification and characterization of FtsZ from the citrus canker pathogen <i>Xanthomonas citri</i> subsp. <i>citri</i>

Kopacz, Malgorzata; Lorenzoni, André Soibelmann Glock; Polaquini, Carlos Roberto; Regasini, Luís Octávio; Scheffers, Dirk‐Jan

doi:10.1002/mbo3.706

Cited by 2 publications

(3 citation statements)

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“…The cell division process is mediated by the formation of the Z ring, coordinated by GTP hydrolysis activity of FtsZ [ 33 , 34 , 35 ]. Because of this, we evaluated the inhibition of GTPase activity of purified FtsZ from Xcc, incubated with compounds 4 , 16 and 28 using the continuous substrate regeneration assay [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

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Design of Antibacterial Agents: Alkyl Dihydroxybenzoates against Xanthomonas citri subsp. citri

Nazaré

Polaquini

Cavalca

et al. 2018

IJMS

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Xanthomonas citri subsp. citri (Xcc) causes citrus canker, affecting sweet orange-producing areas around the world. The current chemical treatment available for this disease is based on cupric compounds. For this reason, the objective of this study was to design antibacterial agents. In order to do this, we analyzed the anti-Xcc activity of 36 alkyl dihydroxybenzoates and we found 14 active compounds. Among them, three esters with the lowest minimum inhibitory concentration values were selected; compounds 4 (52 μM), 16 (80 μM) and 28 (88 μM). Our study demonstrated that alkyl dihydroxybenzoates cause a delay in the exponential phase. The permeability capacity of alkyl dihydroxybenzoates in a quarter of MIC was compared to nisin (positive control). Compound 28 was the most effective (93.8), compared to compound 16 (41.3) and compound 4 (13.9) by percentage values. Finally, all three compounds showed inhibition of FtsZ GTPase activity, and promoted changes in protofilaments, leading to depolymerization, which prevents bacterial cell division. In conclusion, heptyl dihydroxybenzoates (compounds 4, 16 and 28) are promising anti-Xcc agents which may serve as an alternative for the control of citrus canker.

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Section: Resultsmentioning

confidence: 99%

“…The evaluation of the inhibition of GTPase activity was measured by the characterization of the FtsZ from Xcc [ 36 , 57 ]. This methodology is known as continuous assay of substrate regeneration and consists of the hydrolysis of guanosine triphosphate (GTP) in guanosine diphosphate (GDP) and inorganic phosphate.…”

Section: Methodsmentioning

confidence: 99%

Design of Antibacterial Agents: Alkyl Dihydroxybenzoates against Xanthomonas citri subsp. citri

Nazaré

Polaquini

Cavalca

et al. 2018

IJMS

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“…rpfF is implicated in diffusible extracellular factor (DSF) formation, while gumD is responsible for extracellular polysaccharide (EPS) biosynthesis, both of which are required for bacterial virulence ( Feng et al., 2023 ). ftsZ is associated with cell division and influences cell metabolism ( Kopacz et al., 2019 ). glmS encodes glucosamine 6-phosphate synthetase that is important for the biosynthesis of peptidoglycan, a component of the bacterial cell wall ( Klein and Ferre-D’Amare, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

Antagonistic transcriptome profile reveals potential mechanisms of action on Xanthomonas oryzae pv. oryzicola by the cell-free supernatants of Bacillus velezensis 504, a versatile plant probiotic bacterium

Zhou

et al. 2023

Front. Cell. Infect. Microbiol.

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Bacterial leaf streak (BLS) of rice is a severe disease caused by the bacterial pathogen Xanthomonas oryzae pv. oryzicola (Xoc) that has gradually become the fourth major disease on rice in some rice-growing regions in southern China. Previously, we isolated a Bacillus velezensis strain 504 that exhibited apparent antagonistic activity against the Xoc wild-type strain RS105, and found that B. velezensis 504 was a potential biocontrol agent for BLS. However, the underlying mechanisms of antagonism and biocontrol are not completely understood. Here we mine the genomic data of B. velezensis 504, and the comparative transcriptomic data of Xoc RS105 treated by the cell-free supernatants (CFSs) of B. velezensis 504 to define differentially expressed genes (DEGs). We show that B. velezensis 504 shares over 89% conserved genes with FZB42 and SQR9, two representative model strains of B. velezensis, but 504 is more closely related to FZB42 than SQR9, as well as B. velezensis 504 possesses the secondary metabolite gene clusters encoding the essential anti-Xoc agents difficidin and bacilysin. We conclude that approximately 77% of Xoc RS105 coding sequences are differentially expressed by the CFSs of B. velezensis 504, which significantly downregulates genes involved in signal transduction, oxidative phosphorylation, transmembrane transport, cell motility, cell division, DNA translation, and five physiological metabolisms, as well as depresses an additional set of virulence-associated genes encoding the type III secretion, type II secretion system, type VI secretion system, type IV pilus, lipopolysaccharides and exopolysaccharides. We also show that B. velezensis 504 is a potential biocontrol agent for bacterial blight of rice exhibiting relative control efficiencies over 70% on two susceptible cultivars, and can efficiently antagonize against some important plant pathogenic fungi including Colletotrichum siamense and C. australisinense that are thought to be the two dominant pathogenic species causing leaf anthracnose of rubber tree in Hainan province of China. B. velezensis 504 also harbors some characteristics of plant growth-promoting rhizobacterium such as secreting protease and siderophore, and stimulating plant growth. This study reveals the potential biocontrol mechanisms of B. velezensis against BLS, and also suggests that B. velezensis 504 is a versatile plant probiotic bacterium.

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Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri

Cited by 2 publications

References 53 publications

Design of Antibacterial Agents: Alkyl Dihydroxybenzoates against Xanthomonas citri subsp. citri

Design of Antibacterial Agents: Alkyl Dihydroxybenzoates against Xanthomonas citri subsp. citri

Antagonistic transcriptome profile reveals potential mechanisms of action on Xanthomonas oryzae pv. oryzicola by the cell-free supernatants of Bacillus velezensis 504, a versatile plant probiotic bacterium

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