Purification and characterization of Heteroscorpine-1 (HS-1) toxin from Heterometrus laoticus scorpion venom

Uawonggul, Nunthawun; Thammasirirak, Sompong; Chaveerach, Arunrat; Arkaravichien, Tarinee; Bunyatratchata, Wandee; Ruangjirachuporn, Wipaporn; Jearranaiprepame, Pornpimol; Nakamura, Takeshi; Matsuda, Minoru; Kobayashi, Minatsu; Hattori, Satoshi; Daduang, Sakda

doi:10.1016/j.toxicon.2006.09.003

Cited by 68 publications

(49 citation statements)

References 19 publications

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“…After 30 minutes, non-paralyzed crickets were counted, and the PD 50 value was estimated. The PD 50 was defined as the amount of venom that could paralyze 50% of the venominjected crickets; crickets that could not turnover from the overturned position were considered paralyzed (36).…”

Section: Paralytic Dose 50 (Pd 50 ) Assaymentioning

confidence: 99%

Characterization of the allergen Sol gem 2 from the fire ant venom, Solenopsis geminata

Sukprasert¹,

Uawonggul²,

Jamjanya³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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Sol i 2 is a potent allergen in Solenopsis invicta venom, and most humans exhibit reactivity to it. The Sol gem 2 allergen found in the venom of the Thai tropical fire ant Solenopsis geminata was analysed in the present study. The protein was present in higher amounts than other proteins, as determined by SDS-PAGE, and presumably has allergenic properties similar to those of Sol i 2. Sol gem 2 molecular weight is 28 and 15 kDa, respectively, under non-reducing and reducing conditions, indicating that its native form is a dimer. LC-MS/MS analysis confirmed its similarity to Sol i 2. The mono/dimeric form of Sol gem 2 was determined to be relevant by proteomic approach and immunoblotting. An anti-Sol gem 2 antibody was produced in mice, with a titer greater than 1:800 according to the Western blotting analysis. The Sol gem 2-neutralising activity of this antibody was determined in crickets. The paralytic dose 50 (PD 50 ) of crude S. geminata venom was elevated from 0.18 mg/g of body weight to more than 0.90 mg/g of body weight after preincubation with antibody at a ratio of 1:1. These results suggest that Sol gem 2 plays an important role in mediating the effects of the piperidine derivatives in the venom.Key words: allergy, ant venom, hymenoptera, immunoreactivity. Original PaPer The Journal of Venomous Animals and Toxins including Tropical Diseases ISSN 1678-9199 | 2012 | volume 18 | issue 3 | pages 325-334 INTRODUCTIONVenomous insects such as bees, wasps, hornets, yellow jackets and fire ants belong to the order Hymenoptera. These insects have a stinging apparatus at the tail end of their abdominal segment and still comprise a serious problem in tropical climates. Every year, a large number of people are stung by these insects (1). The stings are capable of delivering between 100 ng (fire ants) and 50 μg (bees) of venom (2, 3). The venom is injected from a gland through the stinger and is used to catch prey and to defend against predators. The venom of these insects is composed of various biologically active peptide and protein components, some of which can cause toxicity or anaphylaxis in humans (4).Fire ants (Solenopsis sp.) are members of the Formicidae family. The medically important aggressive species are S. invicta, S. richteri, S. geminata and S. saevissima (5, 6). The venom components of these ants and their immunoreactivity have been extensively studied. Approximately 95% of fire ant venom consists of water-insoluble piperidine alkaloids, and approximately 0.1% of its weight (10 to 100 ng of protein in 0.04 to 0.11 μL per sting) is protein (7). The toxicity of the venom is thought to be due to solenopsins and methyl-, alkylor alkenyl-substituted piperidines (7-9). The venom has cytotoxic, insecticidal, antibiotic and antimicrobial properties as well (7,(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). The protein components of venom, which are wellknown allergens, are believed to play important roles in accelerating the spread of solenopsins to nearby cells and tissues. Solenopsins are believed t...

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Section: Paralytic Dose 50 (Pd 50 ) Assaymentioning

confidence: 99%

Characterization of the allergen Sol gem 2 from the fire ant venom, Solenopsis geminata

Sukprasert¹,

Uawonggul²,

Jamjanya³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Scanning electron microscopy (SEM) was performed by the method of Uawonggul et al (2007) with slight modification. After the bacteria were grown in agar plates for 24 h, the cells at inhibition zone were harvested.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

High phenolics and antioxidants of some tropical vegetables related to antibacterial and anticancer activities

Daduang¹

2011

Afr. J. Pharm. Pharmacol.

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Phytochemicals play an important role in the decrease risk of free radicals associated diseases. In the present study, total phenolic contents, antioxidant, antibacterial and anticancer activities of thirty Thai vegetables have been evaluated. The results showed that phenolic contents of Thai vegetables were highly correlated with total antioxidant activities. Four vegetables in strains of Spondias pinnata Kurz., Careya sphaerica Roxb., Caesalpinia mimosoides Lamk. and Cratoxylum Subsp. Pruniflorum (Kurz.) Gogel. with high phenolic contents and total antioxidant activities, have strong antibacterial activities against Staphylococcus aureus, Salmonella typhimurium and Eschericia coli. Although, the extracted samples were non-cytotoxic as confirmed on the normal Vero cells, only C. mimosoides Lamk. extract had inhibitory effect on oral cavity cell line (IC 50 27.17 ± 5.44 µg/ml). Thus, this study suggested that some vegetables can be considered as possible therapeutic agents.

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“…The bacterial membrane was investigated after treatment with AuNP-GA by scanning electron microscope (SEM), which was performed according to the method described in Uawonggul et al, 31 with slight modification. P. shigelloides and S. flexneri B were cultured in nutrient broth and harvested at the logarithmic phase of growth by centrifugation at 3,000× g for 5 minutes.…”

Section: Detection Of Bacterial Morphology By Scanning Electron Micromentioning

confidence: 99%

Gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens

Rattanata¹,

Klaynongsruang²,

Leelayuwat

et al. 2016

IJN

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Foodborne pathogens, including Plesiomonas shigelloides and Shigella flexneri B, are the major cause of diarrheal endemics worldwide. Antibiotic drug resistance is increasing. Therefore, bioactive compounds with antibacterial activity, such as gallic acid (GA), are needed. Gold nanoparticles (AuNPs) are used as drug delivery agents. This study aimed to conjugate and characterize AuNP–GA and to evaluate the antibacterial activity. AuNP was conjugated with GA, and the core–shell structures were characterized by small-angle X-ray scattering and transmission electron microscopy. Antibacterial activity of AuNP–GA against P. shigelloides and S. flexneri B was evaluated by well diffusion method. AuNP–GA bactericidal mechanism was elucidated by Fourier transform infrared microspectroscopic analysis. The results of small-angle X-ray scattering showed that AuNP–GA conjugation was successful. Antibacterial activity of GA against both bacteria was improved by conjugation with AuNP because the minimum inhibitory concentration value of AuNP–GA was significantly decreased ( P <0.0001) compared to that of GA. Fourier transform infrared analysis revealed that AuNP–GA resulted in alterations of lipids, proteins, and nucleic acids at the bacterial cell membrane. Our findings show that AuNP–GA has potential for further application in biomedical sciences.

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Purification and characterization of Heteroscorpine-1 (HS-1) toxin from Heterometrus laoticus scorpion venom

Cited by 68 publications

References 19 publications

Characterization of the allergen Sol gem 2 from the fire ant venom, Solenopsis geminata

Characterization of the allergen Sol gem 2 from the fire ant venom, Solenopsis geminata

High phenolics and antioxidants of some tropical vegetables related to antibacterial and anticancer activities

Gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens

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